LOCUS       sequence-315034-        8720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR115-Tango
FEATURES             Location/Qualifiers
     source          1..8720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3044..3658,3659..4045)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3111..3130)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4077..4094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4083..4307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4353..4781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4440..4459)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4650..4671
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4790..4810)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4795..5124
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4975..5110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5037..5056
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5191..5985
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5245..5264)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5855..5874
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6159..6280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6196..6215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6250..6269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6329..6345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6329..6345)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6342..6364)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6353..6369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6377..6383,6384..6401,6402..6407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6422..6443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6560..6577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6731..7319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6811..6830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7490..8281,8282..8350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8113..8132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8351..8455)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8530..8549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 gaaaatgaag agccaggcta caatgatttg ttgtctggtg ttctttctga gtacagagtg
      901 tagccactac cgcagcaaga tccatcttaa ggcaggagac aagctgcagt caccagaagg
      961 aaagcccaag accggcagaa ttcaggaaaa atgtgagggt ccgtgtatct ctagctccaa
     1021 ctgctcacag ccttgtgcaa aggatttcca cggtgaaatt ggcttcacat gtaatcaaaa
     1081 gaaatggcag aagtctgccg agacatgcac cagcttgagt gtcgagaaac tgtttaagga
     1141 ttccaccggg gcgtctcgct tgagtgtcgc agcaccatca atcccactgc atattctgga
     1201 cttccgggct cccgagacta tagaaagtgt ggcacaaggg atccggaaaa attgcccatt
     1261 tgactatgct tgtataaccg atatggtcaa atcatctgaa accactagcg ggaatattgc
     1321 ctttatcgta gagctgctga aaaacatctc aacagatctc tccgacaatg tgacacgcga
     1381 gaaaatgaaa tcctactctg aggtggctaa tcatatactg gacacagcag ccatctccaa
     1441 ttgggccttc attcctaaca agaatgctag ttctgacctt ctgcagtctg tgaatctctt
     1501 cgctaggcag cttcacattc acaataatag cgagaacatc gtcaatgagc tttttattca
     1561 gactaaggga ttccatatca atcataatac ctccgaaaaa tctcttaact ttagtatgtc
     1621 catgaacaac accaccgagg acatcttggg catggtccaa attcctaggc aagagttgag
     1681 gaaactttgg ccaaatgcta gccaggcgat cagcatcgcc ttcccaactc tgggagccat
     1741 tctgcgcgaa gcacatcttc aaaacgtctc tctgcccagg caggttaatg gtctggtcct
     1801 ctctgttgtg ttgcctgaac gcctgcagga aataatactc acgttcgaga aaattaataa
     1861 aacaaggaat gctcgcgcac agtgcgtggg ctggcattcc aagaagaggc ggtgggatga
     1921 gaaggcctgt cagatgatgt tggacattcg gaatgaagtc aagtgtcggt gcaattacac
     1981 cagcgtcgtg atgagcttct caattcttat gagcagcaaa tccatgaccg acaaggtgct
     2041 cgactacatc acatgcatcg gcctctctgt ttctatcctt tcacttgtcc tgtgcttgat
     2101 catcgaggca accgtctggt cacgagttgt tgtcaccgaa atctcataca tgaggcacgt
     2161 gtgtatcgta aatattgctg tctccctcct gacagcaaac gtttggttca ttattggatc
     2221 acatttcaat ataaaggccc aggattacaa tatgtgcgtg gctgttacct tcttctccca
     2281 cttcttctat ctcagcctgt ttttttggat gctctttaag gccctgctca tcatctatgg
     2341 gatactggtg atattcagaa ggatgatgaa aagccgaatg atggttattg gcttcgccat
     2401 aggatacggc tgcccactta ttatcgcggt gacaactgtc gcgataacag agcccgagaa
     2461 aggttatatg cgacctgaag cctgttggct gaactgggac aacactaagg ccctgcttgc
     2521 tttcgctata cccgcctttg tgatcgttgc ggtgaatctc atcgtggtac tggtggtggc
     2581 tgtcaataca cagaggccct ctatcgggtc tagtaagtct caggatgttg taatcattat
     2641 gagaatcagt aagaacgtcg ccattcttac ccctcttctt ggccttacat ggggatttgg
     2701 cattgctact ctcattgagg gaacgagcct cactttccac atcatcttcg cactgctgaa
     2761 tgcatttcag ggcttcttca ttcttctgtt cggcactatc atggaccata agatccgcga
     2821 cgcccttcgc atgaggatga gcagtctgaa gggaaagagc cgagccgccg agaacgcctc
     2881 ccttgggcct accaacggct ccaagctcat gaaccggcag ggcatcgata ccggtggacg
     2941 caccccaccc agcctgggtc cccaagatga gtcctgcacc accgccagct cctccctggc
     3001 caaggacact tcatcgaccg gtgagaacct gtacttccag ctaagattag ataaaagtaa
     3061 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     3121 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     3181 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     3241 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     3301 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     3361 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     3421 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     3481 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     3541 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     3601 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
     3661 gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct
     3721 cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc
     3781 cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga
     3841 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
     3901 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc
     3961 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
     4021 tggaattgac gagtacggtg ggtagtctag agggcccgtt taaacccgct gatcagcctc
     4081 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4141 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4201 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4261 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4321 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4381 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4441 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4501 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4561 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4621 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4681 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4741 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     4801 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4861 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4921 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4981 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5041 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5101 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5161 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     5221 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5281 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5341 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5401 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5461 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5521 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5581 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5641 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5701 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5761 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5821 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5881 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5941 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6001 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6061 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6121 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6181 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6241 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6301 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6361 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6421 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6481 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6541 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8461 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     8521 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8581 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8641 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     8701 acgggccaga tatacgcgtt
//
