LOCUS       sequence-315035-        6145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-Host range expression vector, pUC origin, Tn7 recognition 
            sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance 
            Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Schuster LA, Reisch CR
  TITLE     A plasmid toolbox for controlled gene expression across the 
            Proteobacteria.
  JOURNAL   Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 
            10.1093/nar/gkab496.
  PUBMED    34125913
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496."
COMMENT     Sequence Label: pILxR5
FEATURES             Location/Qualifiers
     source          1..6145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..28
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12..28
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        1006..1055
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             1100..1111
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1118..1795
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     mobile_element  1875..2040
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(2310..2898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2390..2409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3069..3860,3861..3929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3692..3711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3930..4034)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4102..4120
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4158..4180)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4280..4299
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4713..4729
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4714..4730
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4758..4852
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      4955..5041
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    5087..5134
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(5264..5797)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5986..5991,5992..6008,6009..6014))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    6055..6102
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtcacgacgt tgtaaaacga cggccagtga tcaattttta aagtatgggc aatcaattgc
       61 tcctgttaaa attgctttag aaatactttg gcagcggttt gttgtattga gtttcatttg
      121 cgcattggtt aaatggaaag tgacagtacg ctcactgcaa cctaatattt ttgaaatatc
      181 ccaagagctt tttccttcgc atgcccacgc taaacattct ttttctcttt tggttaaatc
      241 gttgtttgat ttattatttg ctatatttat ttttcgataa ttatcaacta gagaaggaac
      301 aattaatggt atgttcatac acgcatgtaa aaataaacta tctatatagt tgtctttttc
      361 tgaatgtgca aaactaagca ttccgaagcc attgttagcc gtatgaatag ggaaactaaa
      421 cccagtgata agacctgatg ttttcgcttc tttaattaca tttggagatt ttttatttac
      481 agcattgttt tcaaatatat tccaattaat tggtgaatga ttggagttag aataatctac
      541 tataggatca tattttatca aattagcgtc atcataatat tgcctccatt ttttagggta
      601 attatctagg attgaaatat cagatttaac catagaatga ggataaatga tcgcgagtaa
      661 ataatattca caatgtacca ttttagtcat atcagataag cattgattaa tatcattatt
      721 gcttctacaa gctttaattt tattaattat tctgtatgtg tcgtcggcat ttatgttttt
      781 cattctgccc ctccgaaagg cttaagtcct cgcgtagtac ctgggcgcta tcatgccata
      841 ccgcgaaagg ttttgcgcca ttcgatggcg cgccgcccaa ttattgaagg cctccctaac
      901 ggggggcctt tttttgtttc tggtctcccg cttaacgatc gttggctgac ctgtaggatc
      961 gtacaggttt acgcaagaaa atggtttgtt acagtcgaat aaaagctgtc accggatgtg
     1021 ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaaga
     1081 actgggaaga gcgcagatta aagaggagaa atactagatg gcttcctccg aagacgttat
     1141 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     1201 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     1261 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     1321 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     1381 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     1441 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     1501 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     1561 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     1621 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     1681 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     1741 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
     1801 ctgatagtgc tagtgtagat cgcgctttcc agtcgggaaa cctgtcgtgg tacctcgcga
     1861 aggccttgca ggccaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa
     1921 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa
     1981 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca
     2041 gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg ccaacgcgcg
     2101 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga cccgctgcgc
     2161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2221 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2341 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2401 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2461 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2521 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2581 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2641 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2701 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2761 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2881 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2941 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3001 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3061 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3361 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3661 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3961 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4021 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     4081 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     4141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4261 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     4321 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     4381 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagcta
     4441 gaggaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg
     4501 cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg
     4561 acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt
     4621 gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa
     4681 attgcccgtc gtattaaaga ggggcgtggg gttcgaggtc gacggtatcg ataagctagc
     4741 ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag aactccatct
     4801 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta
     4861 gactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
     4921 acgcctgggg taatgactct ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa
     4981 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     5041 tccgccgcta ggagcttgcg gcccggacga tcgaattggg gatcttgaag tacctattcc
     5101 gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgtgctt
     5161 aaaaacttac tcaatggaat tctagatcga attggccgcg gcgttgtgac aatttaccga
     5221 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     5281 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     5341 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     5401 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     5461 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     5521 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     5581 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     5641 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     5701 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     5761 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5821 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     5881 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     5941 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     6001 caggcttatg tcaattcgat ccattgctgt tgacaaaggg aatcagggga tcttgaagtt
     6061 cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagcta
     6121 attcgatcat gcatgagctc actag
//
