LOCUS       sequence-31504-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB1A
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(714..732)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cctctgaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggttccgc gtggtagtcc cgaatatgat tatttattca
      121 agttacttct gattggcgac tcaggggttg gaaagtcttg ccttcttctt aggtttgcag
      181 atgatacata tacagaaagc tacatcagca caattggtgt ggatttcaaa ataagaacta
      241 tagagttaga cgggaaaaca atcaagcttc aaatatggga cacagcaggc caggaaagat
      301 ttcgaacaat cacctccagt tattacagag gagcccatgg catcatagtt gtgtatgatg
      361 tgacagatca ggagtccttc aataatgtta aacagtggct gcaggaaata gatcgttatg
      421 ccagtgaaaa tgtcaacaaa ttgttggtag ggaacaaatg tgatctgact acaaagaaag
      481 tagtagacta cacaacagcg aaggaatttg ctgattccct tggaattccg tttttggaaa
      541 ccagtgctaa gaatgcaacg aatgtagaac agtctttcat gacgatggca gctgagatta
      601 aaaagcgaat gggttgatga cgaagcttgc ggccgcactc gagcaccacc accaccacca
      661 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      721 gcaataacta gcataacccc ttg
//
