LOCUS       sequence-31506-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARL10B:L9-K182
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             653..670
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tccctctaga ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag tctggactgg ttcaaggccc
      121 tattctggaa ggaggagatg gagctcacgc tggtcgggct tcagtactcg ggcaagacca
      181 ccttcgtcaa cgtgatcgcg tcaggacagt tcaacgagga catgatcccc accgtgggtt
      241 tcaacatgcg caaaatcacc aaagggaatg tgactatcaa gctctgggac attgggggac
      301 agccgcgttt ccgcagcatg tgggagcgct actgccgagg agtgagcgcc atcgtgtaca
      361 tggtggatgc tgctgaccag gagaagattg aggcctctaa gaacgagctc cacaacctac
      421 tggacaaacc tcagctgcag ggcatcccgg tcttagtcct gggtaacaag cgagaccttc
      481 cgggagcatt ggatgagaag gagctgattg agaaaatgaa tctgtctgcc atccaggacc
      541 gagagatctg ctgctactcc atctcttgca aagaaaagga caacattgac atcaccctac
      601 agtggcttat tcaacactcg aagtgatgac gaagcttgcg gccgcactcg agcaccacca
      661 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      721 caccgctgag caataactaa gcataacccc cttggggcct ctaaacgggt cttga
//
