LOCUS       sequence-315068-        3981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfYFP tagged with ssrA from the Prhl promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  TITLE     Bennett Lab Plasmids and Strains
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDZ113
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..32
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     21..43
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             234..263
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    448..464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             549..1262
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1448..1475
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1624..2439)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2347..2366
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2431..2450
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2561..3149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3050..3069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3303..3320
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3335..3474
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3400..3419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3560..3582
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3576..3767)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gacgtctgtg tggaattgtg agcggataac aatttcacac agggccctcg gacaccgagg
       61 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
      121 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
      181 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
      241 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
      301 aacagctacg gaactcttgt gcgtaaggaa aagtaaggaa aacgattcct tctaacagaa
      361 atgtcctgag caatcaccta tgaactgtcg atcctgtgaa atctggcagt tagacatgtg
      421 agcgctcaca atttatagtg agcacaattg tgagcggata acaattgcat gccacttaaa
      481 aaggagatca acaatgaaag caattttcgt actgaaacat cttaatcatg ctaaggagaa
      541 atactagtat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac
      601 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa
      661 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc
      721 cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata
      781 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga
      841 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata
      901 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg
      961 gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa
     1021 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc
     1081 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca
     1141 atcactatct gagctaccaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata
     1201 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca
     1261 aagcagcgaa cgacgaaaat tacgcccttg cagcgtaata aaagcttccc ctacgtgctg
     1321 ctgaagttgc ccgcaacaga gagtggaacc aaccggtgat accacgatac tatgactgag
     1381 agtcaacgcc atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgtc
     1441 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
     1501 aaagccagat tattaatccg gcttttttat tatttcctag ctagccgggg aaatgtgcgc
     1561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
     1621 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     1681 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     1741 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     1801 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     1861 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     1921 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     1981 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     2041 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     2101 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     2161 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     2221 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     2281 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     2341 gacattatcg cgagcccatt tatacccata taaatcagca tccatgctgg aatttaatcg
     2401 cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     2461 tatgtaagca gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2701 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3241 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3301 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3361 atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc
     3421 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     3481 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3541 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3601 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     3661 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     3721 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     3781 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     3841 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     3901 gcggtatgga tgcggcggga ccagagctca gtgcgctcga gcttcccaac cttaccagag
     3961 ggcgccccag ctggcaattc c
//
