LOCUS       sequence-315069-        5276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfYFP tagged with ssrA from the PlLacO1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5276)
  TITLE     Bennett Lab Plasmids and Strains
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDZ115
FEATURES             Location/Qualifiers
     source          1..5276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             219..248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    383..399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    414..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             514..1227
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1424..1495
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1705..1896
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1890..1912)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1998..2137
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2053..2072
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2152..2169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2323..2911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2403..2422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3022..3041)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3033..3848
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3106..3125)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4511..4538)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacagggc cctcggacac cgaggagaat gtcaagaggc
       61 gaacacacaa cgtcttggag cgccagagga ggaacgagct aaaacggagc ttttttgccc
      121 tgcgtgacca gatcccggag ttggaaaaca atgaaaaggc ccccaaggta gttatcctta
      181 aaaaagccac agcatacatc ctgtccgtcc aagcagagga gcaaaagctc atttctgaag
      241 aggacttgtt gcggaaacga cgagaacagt tgaaacacaa acttgaacag ctacggaact
      301 cttgtgcgta aggaaaagta aggaaaacga ttccttctaa cagaaatgtc ctgagcaatc
      361 acctatgaac tgtcgattga cattgtgagc ggataacaag atactgagca caattgtgag
      421 cggataacaa ttgcatgcca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg
      481 aaacatctta atcatctaag gagaaatact agtatgcgta aaggcgaaga gctgttcact
      541 ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg
      601 cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact
      661 actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag
      721 tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg
      781 gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt
      841 gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa aggcattgac
      901 tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat
      961 gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa aattcgccac
     1021 aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt
     1081 gatggtcctg ttctgctgcc agacaatcac tatctgagct accaaagcgt tctgtctaaa
     1141 gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc
     1201 acgcatggta tggatgaact gtacaaagca gcgaacgacg aaaattacgc ccttgcagcg
     1261 taataagagc tcgaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
     1321 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
     1381 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     1441 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1501 gagtcataag tgcggaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     1561 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     1621 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     1681 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc
     1741 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     1801 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     1861 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     1921 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1981 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     2041 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattgc ggtgtgaaat
     2101 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     2161 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2221 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2761 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca ataaaactgt
     3001 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt
     3061 gctctaggcc gcgattaaat tccagcatgg atgctgattt atatgggtat aaatgggctc
     3121 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
     3181 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     3241 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     3301 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat
     3361 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     3421 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     3481 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     3541 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     3601 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     3661 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     3721 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     3781 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     3841 ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3901 ataggggttc cgcgcacatt tccccggcta gccgcctggt gctacgcctg aataagtgat
     3961 aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc
     4021 agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa
     4081 gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg
     4141 aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata aaaacggtta
     4201 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     4261 gatccggaac ataatggtgc agggcgcttg tttcggcgtg ggtatggtgg caggccccgt
     4321 ggccggggga ctgttgggcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     4381 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc taccgcctgc
     4441 ggccgctagg aaataataaa aaagccggat taataatctg gctttttata ttctctctct
     4501 agtatataaa cgcagaaagg cccacccgaa ggtgagccag tgtgagacag cggtgcggac
     4561 tgttgtaact cagaataaga aatgaggccg ctcatggcgt tgactctcag tcatagtatc
     4621 gtggtatcac cggttggttc cactctctgt tgcgggcaac ttcagcagca cgtaggggaa
     4681 gcttttatta ctcgagaagc gcgtctgaat aggcttcttg tattcctgcc acacagggaa
     4741 accatcagga gttacccaat gcacagcgca acgcttgcga agaatctctc cagtcttctt
     4801 atctttgacc tcagcagcca gcagcttagc agcagactta agccagttca ttgcttcaac
     4861 cgcagctacc accgtcacgc tcacagattc ccaaatcagc ttagccatgt atccagcagc
     4921 ctgattcggc tgagtgaaca tcagaccctt gccggaatca atagctggct gaatggtatc
     4981 ttccagcact tgttgacgga agccgaactc tttggacccg taagccagcg tcatgactga
     5041 acgcttagtc acactgcgag taacaccgta agccagccat tgaccagcca gtgccttagt
     5101 gcccagcttg actttctcag agatttcacc agtgttctca tcggtcacgg taactacttc
     5161 gttatcggtc ccattgattg cgtctgcttg tagaatctcg ttgactttct tagcaacaat
     5221 cccgtagatg tcctgaacgg tttcactagg aagcaagtta agacgtctgt gtggaa
//
