LOCUS       sequence-315073-        8495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of scFLARE1 component bearing a p2A-eGFP as 
            transfection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Sanchez MI, Nguyen QA, Wang W, Soltesz I, Ting AY
  TITLE     Transcriptional readout of neuronal activity via an engineered 
            Ca(2+)-activated protease.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 
            10.1073/pnas.2006521117.
  PUBMED    33323488
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 
            10.1073/pnas.2006521117."
COMMENT     Sequence Label: scFLARE1-p2A-eGFP
FEATURES             Location/Qualifiers
     source          1..8495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      585..594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     591..650
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1416..1925
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             3612..3632
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             3645..3668
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3669..4106
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4107..4448
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             4449..4490
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4500..4556
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4563..4565,4566..4568,4569..5282)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4563..4565,4566..4568,4569..5279)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4608..4629)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4869..4888)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5216..5237
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5316..5333)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5322..5529
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5558..5698
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5558..5687
                     /label=AAV2 ITR
     rep_origin      5773..6228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5860..5879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6070..6091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6245..6264)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6364..6386
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6424..6442)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6510..6614
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6615..6683,6684..7475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6833..6852)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7646..8234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8135..8154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   8296..8425
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc atggagacag
      601 acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt gacggtgccc
      661 agccggccct cgacttccag aaggcctcca gcatagtcta taagaaagag ggggaacagg
      721 tggagttctc cttcccactc gcctttacag ttgaaaagct gacgggcagt ggcgagctgt
      781 ggtggcaggc ggagagggct tcctcctcca agtcttggat cacctttgac ctgaagaaca
      841 aggaagtgtc tgtaaaacgg gttacccagg accctaagct ccagatgggc aagaagctcc
      901 cgctccacct caccctgccc caggccttgc ctcagtatgc tggctctgga aacctcaccc
      961 tggcccttga agcgaaaaca ggaaagttgc atcaggaagt gaacctggtg gtgatgagag
     1021 ccactcagct ccagaaaaat ttgacctgtg aggtgtgggg acccacctcc cctaagctga
     1081 tgctgagctt gaaactggag aacaaggagg caaaggtctc gaagcgggag aaggcggtgt
     1141 gggtgctgaa ccctgaggcg gggatgtggc agtgtctgct gagtgactcg ggacaggtcc
     1201 tgctggaatc caacatcaag gttctgccca catggtccac cccggtgcag ccaatggccc
     1261 tgattgtgct ggggggcgtc gccggcctcc tgcttttcat tgggctaggc atcttcttct
     1321 gtgtcaggtg ccggcaccga aggcgcaagg gctcgggctc gacctcgggc tcgggcagcg
     1381 gtggctctag aggttctggt ggcagctctg gaggtatgaa tggagctata ggaggtgacc
     1441 ttttgctcaa ttttcctgac atgtcggtcc tagagcgcca aagggctcac ctcaagtacc
     1501 tcaatcccac ctttgattct cctctcgccg gcttctttgc cgattcttca atgattaccg
     1561 gcggcgagat ggacagctat ctttcgactg ccggtttgaa tcttccgatg atgtacggtg
     1621 agacgacggt ggaaggtgat tcaagactct caatttcgcc ggaaacgacg cttgggactg
     1681 gaaatttcaa ggcagcgaag tttgatacag agactaagga ttgtaatgag gcggcgaaga
     1741 agatgacgat gaacagagat gacctagtag aagaaggaga agaagagaag tcgaaaataa
     1801 cagagcaaaa caatgggagc acaaaaagca tcaagaagat gaaacacaaa gccaagaaag
     1861 aagagaacaa tttctctaat gattcatcta aagtgacgaa ggaattggag aaaacggatt
     1921 atattcatgg tggctctggt agcggagaaa gcttgtttaa gggaccacgt gattacaacc
     1981 cgatatcgag caccatttgt catttgacga atgaatctga tgggcacaca acatcgttgt
     2041 atggtattgg atttggtccc ttcatcatta caaacaagca cttgtttaga agaaataatg
     2101 gaacactgtt ggtccaatca ctacatctcg aggaccaact gactgaagag cagatcgcag
     2161 aatttaaaga ggctttctcc ctatttgaca aggacgggga tgggacaata acaaccaagg
     2221 agctggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag ctgcaggaca
     2281 tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag ttcctgacaa
     2341 tgatggcaag aaaaatgaaa tacacaggcg gtgtgaagct gatcccctcc tggaccaccg
     2401 tgatcctggt gaagagcatg ctgcgcaagc gcagcttcgg caaccccttc ggtggcgaca
     2461 gtgaagaaga aattagagaa gcgttccgtg tgtttgataa ggatggcaat ggctacatca
     2521 gtgcagcaga gcttcgccac gtgatgacaa accttggaga gaagttaaca gatgaagagg
     2581 ttgatgaaat gatcagggaa gcagacatcg atggggatgg tcaggtaaac tacgaagagt
     2641 ttgtacaaat gatgacagcg aaggcggccg cgggtgtatt caaggtcaag aacaccacga
     2701 ctttgcaaca acacctcatt gatgggaggg acatgataat tattcgcatg cctaaggatt
     2761 tcccaccatt tcctcaaaag ctgaaattta gagagccaca aagggaagag cgcatatgtc
     2821 ttgtgacaac caacttccaa actaagagca tgtctagcat ggtgtcagac actagttgca
     2881 cattcccttc atctgatggc atattctgga agcattggat tcaaaccaag gatgggcagt
     2941 gtggcaatcc attagtatca actagagatg ggttcattgt tggtatacac tcagcatcga
     3001 atttcaccaa cacaaacaat tatttcacaa gcgtgccgaa aaacttcatg gaattgttga
     3061 caaatcagga ggcgcagcag tgggttagtg gttggcgatt aaatgctgac tcagtattgt
     3121 ggggtggcca taaagttttc atggtggagc tggccgagaa gctggccggc ctggacatca
     3181 acggtggagc tagcgagttt cgggcaacca cactggaacg gatcgagaaa agcttcgtga
     3241 ttactgatcc gagactgcct gacaacccaa tcatttttgt gagcgattcc ttcctgcagc
     3301 tgacagaata ttctcgggaa gagatcctgg ggcgcaattg ccgttttctg cagggacccg
     3361 agacagaccg tgccactgtt cggaaaatca gagatgctat tgacaaccag actgaagtga
     3421 ccgttcagct gatcaattat accaagagcg gcaagaagtt ctggaacgtg ttccacctgc
     3481 agccgatgcg cgattataag ggcgacgtcc agtacttcat tggcgtgcag ctggatggca
     3541 ccgaacgtct tcatggcgcc gctgagcgtg aggcggtctg cctggtcaaa aagacagcct
     3601 ttcagattgc tgagaacctg tacttccaga tgggcggagg atccgactac aaggatgacg
     3661 acgataagaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
     3721 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
     3781 gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat
     3841 caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca
     3901 tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac
     3961 aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc
     4021 ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca
     4081 aaggtcaaag acagttgact gtatcggcca attttaatca aagtgggaat attgctgata
     4141 gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata acaactcaaa
     4201 caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat aacttcatga
     4261 ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca ctgtcacctg
     4321 gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg tttaatacca
     4381 ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca ccaaacccaa
     4441 aaaaagaggg caagccgatc cctaaccctc tgctgggcct ggacagcact ggaagcggag
     4501 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctgaat
     4561 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4621 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4681 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4741 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4801 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4861 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4921 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4981 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5041 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5101 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5161 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5221 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5281 agtaagaatt cctcgagcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     5341 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5401 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5461 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5521 tgctggggag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     5581 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     5641 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     5701 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     5761 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5821 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     5881 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5941 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     6001 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6061 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     6121 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     6181 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     6241 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6301 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6361 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6421 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6481 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7321 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8281 cttttgctca catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg
     8341 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
     8401 gccaactcca tcactagggg ttcctgcggc cgcacgcgtt gtacatagta atcaattacg
     8461 gggtcattag ttcatagccc atatatggag ttccg
//
