LOCUS       sequence-315076-        5296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfYFP tagged with ssrA from the Pcin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5296)
  TITLE     Bennett Lab Plasmids and Strains
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDZ114
FEATURES             Location/Qualifiers
     source          1..5296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             219..248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    433..449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             534..1247
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1444..1515
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1725..1916
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1910..1932)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2018..2157
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2073..2092
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2172..2189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2343..2931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2423..2442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3042..3061)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3053..3868
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3126..3145)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4531..4558)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacagggc cctcggacac cgaggagaat gtcaagaggc
       61 gaacacacaa cgtcttggag cgccagagga ggaacgagct aaaacggagc ttttttgccc
      121 tgcgtgacca gatcccggag ttggaaaaca atgaaaaggc ccccaaggta gttatcctta
      181 aaaaagccac agcatacatc ctgtccgtcc aagcagagga gcaaaagctc atttctgaag
      241 aggacttgtt gcggaaacga cgagaacagt tgaaacacaa acttgaacag ctacggaact
      301 cttgtgcgta aggaaaagta aggaaaacga ttccttctaa cagaaatgtc ctgagcaatc
      361 acctatgaac tgtcgagggg gcctatctga gggaatttac ttgtgagcgc tcacaattga
      421 tactgagcac aattgtgagc ggataacaat tgcatgccac ttaaaaagga gatcaacaat
      481 gaaagcaatt ttcgtactga aacatcttaa tcatgctaag gagaaatact agtatgcgta
      541 aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca
      601 acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga
      661 cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga
      721 cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact
      781 tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg
      841 acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca
      901 ttgagctgaa aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat
      961 acaattttaa cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag
     1021 cgaattttaa aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc
     1081 agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagct
     1141 accaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt
     1201 tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaagca gcgaacgacg
     1261 aaaattacgc ccttgcagcg taataagagc tcgaacgcag aagcggtctg ataaaacaga
     1321 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     1381 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1501 tcggtgaacg ctctctacta gagtcataag tgcggaagcg ctggcattga ccctgagtga
     1561 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     1621 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     1681 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
     1741 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     1801 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     1861 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     2041 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     2101 gcaccattgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     2161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2761 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3001 gtcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     3061 tattcaacgg gaaacgtctt gctctaggcc gcgattaaat tccagcatgg atgctgattt
     3121 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     3181 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     3241 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     3301 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     3361 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     3421 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     3481 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     3541 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3601 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3661 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3721 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3781 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3841 tcatttgatg ctcgatgagt ttttctaaga attaattcat gagcggatac atatttgaat
     3901 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccggcta gccgcctggt
     3961 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
     4021 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
     4081 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
     4141 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
     4201 gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     4261 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg
     4321 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg
     4381 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     4441 cggaaggagc taccgcctgc ggccgctagg aaataataaa aaagccggat taataatctg
     4501 gctttttata ttctctctct agtatataaa cgcagaaagg cccacccgaa ggtgagccag
     4561 tgtgagacag cggtgcggac tgttgtaact cagaataaga aatgaggccg ctcatggcgt
     4621 tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt tgcgggcaac
     4681 ttcagcagca cgtaggggaa gcttttatta ctcgagaagc gcgtctgaat aggcttcttg
     4741 tattcctgcc acacagggaa accatcagga gttacccaat gcacagcgca acgcttgcga
     4801 agaatctctc cagtcttctt atctttgacc tcagcagcca gcagcttagc agcagactta
     4861 agccagttca ttgcttcaac cgcagctacc accgtcacgc tcacagattc ccaaatcagc
     4921 ttagccatgt atccagcagc ctgattcggc tgagtgaaca tcagaccctt gccggaatca
     4981 atagctggct gaatggtatc ttccagcact tgttgacgga agccgaactc tttggacccg
     5041 taagccagcg tcatgactga acgcttagtc acactgcgag taacaccgta agccagccat
     5101 tgaccagcca gtgccttagt gcccagcttg actttctcag agatttcacc agtgttctca
     5161 tcggtcacgg taactacttc gttatcggtc ccattgattg cgtctgcttg tagaatctcg
     5221 ttgactttct tagcaacaat cccgtagatg tcctgaacgg tttcactagg aagcaagtta
     5281 agacgtctgt gtggaa
//
