LOCUS       sequence-31508-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARL5
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(602..619)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(629..646)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             665..687
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    702..726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(726..745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(727..745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(829..848)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cnnnagcttc
       61 gtcatcatct aatcttaagt cgtgacatca tccattcaag tccttggcac aatccctcgc
      121 cagttagagc acagcatgcc tggatatgcc actggtgatc tttaatagaa gttagcttca
      181 aaaactggga gatttctgct acagtcatgc attctttaac atcttgttta ttagcaaaaa
      241 tcagcaatcc agcttttctt aggtcctcat gcgctaacat tttatagagt tcttctctag
      301 ttacagaaat cctctctctg tctgtactgt ccacaacaac tattacaaac tctgtgttag
      361 tatagtaagt gttccaggaa gaacgaagag attcttggcc accaatatcc cacattagga
      421 aacgtgtatt attaatcact atctcttcta cattacttcc tattgtagga gatgtatgta
      481 caacttcatt catagaaaat tggtaaagaa tggtagtttt ccctgcatta tccagcccaa
      541 caatgataac tttgtgctcc tggtgattga acagtctcca tattctagtg aagagaattc
      601 cactaccacg cggaaccagg ccgctgctgt gatgatgatg atgatggctg ctgcccatgg
      661 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      721 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      781 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      841 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      901 tgggtatggt ggcaggcccc gt
//
