LOCUS       sequence-31509-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARL5:H14-K177 (2H16)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             622..639
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(692..710)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      706..753
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt caccaggagc acaaagttat
      121 cattgttggg ctggataatg cagggaaaac taccattctt taccaatttt ctatgaatga
      181 agttgtacat acatctccta caataggaag taatgtagaa gagatagtga ttaataatac
      241 acgtttccta atgtgggata ttggtggcca agaatctctt cgttcttcct ggaacactta
      301 ctatactaac acagagtttg taatagttgt tgtggacagt acagacagag agaggatttc
      361 tgtaactaga gaagaactct ataaaatgtt agcgcatgag gacctaagaa aagctggatt
      421 gctgattttt gctaataaac aagatgttaa agaatgcatg actgtagcag aaatctccca
      481 gtttttgaag ctaacttcta ttaaagatca ccagtggcat atccaggcat gctgtgctct
      541 aactggcgag ggattgtgcc aaggacttga atggatgatg tcacgactta agtgatgacg
      601 aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa
      661 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      721 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
      781 gcgaatggga cgcgccctgt agcggcgcat ttaagcgcgg cgggtgtggg tggttacgcg
      841 cagcgtgacc gctacacttg ccagcgccct agcg
//
