LOCUS       sequence-315092-       18172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy 
            number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18172)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Miscellaneous gene targeting vector plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK
FEATURES             Location/Qualifiers
     source          1..18172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3974..4036
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           4058..4097
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             4115..5245
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(5243..5262)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5287..5335
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(5639..5656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5639..5655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5648..5670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5864..5883)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5983..6005
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6043..6061)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6129..6233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6234..6302,6303..7094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6452..6471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7259..7804
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     7705..7724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7921..7938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             12264..12317
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             12333..13064
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     protein_bind    complement(15044..15077)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    15132..15356
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     15345..15362
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(15394..16197)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15505..15524)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     16115..16134
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(16216..16263)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(16275..16774)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(16323..16342)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     16716..16739
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(16838..16871)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tctgtgtgtg tatagactac taccgactgt cacttggtgt ttcctatctt taagtgtatg
       61 ttgtcagtgt aatgtctgag gaaatgtctt ttcctctctt ctagagataa ctacttactc
      121 tctaaagtga tctctctgtc tgtccgcagg atgtgtttct gggtttttgt tctttttttt
      181 tttttttttt tttttttgtc tgaggcctca tgtcagccct gctttctgca gagccagcct
      241 accctgggca ttataaacaa ctgtacctta ctgttttctt tcagtccttt acattctgtg
      301 ccacctttgc cttattatat ccgtggctta gtgctaagct ggcactactc catagaaggt
      361 gtagaaggca gctttcggga agcgtagctg agttttgttt gaatcgtgtt tgagcacatt
      421 taagtaaacg ctagatgatg cttcactgta gagctttgaa agactgcctg atgtttcaca
      481 cggcagtgta ggctccagat ggtgtttgta tgttatggac cttactgtgt ttctcgtagc
      541 tgaacaatgc atgtggttat ttgtttgttt gttttttggt ttctctgtgt agccctggct
      601 gtcctggaac tcactctgta gaccaggctg gcctcgaatt cagaaatgcg cctgcctctg
      661 cctcccaagt gctgggatca aaggcgtgcg ccaccacgcc cggctttgca tatggttatc
      721 tgtatggaga agaattagat gtctttcctg agagctttgg aggttgggaa tcatcttctt
      781 ctgtttattt ttatttttaa cattacatta cattcttctg attcagtatc gcaagaaagg
      841 attttttctt ttttcaaaaa caaaacttta agaagccaga ggctaggctg agaatgttct
      901 gcgctcgtcc tcacagctct gctgccgaca ctgccaacgc tcgagacagt cagcctggaa
      961 cctctgggac aaggtctact cgaggtctca cttggacagc gagttctgcc ttgtcattga
     1021 acaaggtcca aaatacagac atttttgcta gggcctagaa atcgaccata aaactcactg
     1081 cattgaggcc taatccattc tatattaaca tccatgtgat aaagtgaaaa taattttgag
     1141 ggttttgctt tcgccaaaaa aaaaaaagaa agaaagaaaa aaaaagaaag gaagaaaaag
     1201 aaaaaagaaa ggattgtttt ccttagcttt gctcaagaaa aatacagcta tcctaaactg
     1261 gaatcttccc gtattcttta gagtcttaag tatagagttt aacttcactt ttgtcagctg
     1321 tcttgagctt atttctaagt agttttgttt tggcttttgg tgagtgaggt agtgggaggt
     1381 taattttggg gtcttttttt tttttggctt tgtttgtgtg tgtgtgtgtg tgtgtgtgtt
     1441 tatgtgtttg gcttttgggt ttggttggtt agttgtttag tttgtttttt tgttgttgtt
     1501 taatttgctt tttctaggca gggtttctct atgtggccct ggctgtccta gaacttgctc
     1561 tgtagaccag gctagcctca aactcagaga tccacttgcc tctgtctcga gtgtgcttga
     1621 actaaaggca tgagctaccc tcctcagccc actccaaact ttgagtaata tttttaaatc
     1681 atagacatgt tggtattaga aacatggcgt attgtggtgg atggtagaga aagggcgccg
     1741 caacctctca ggacatcata tgtctggcca gctcttcatt catataggtg ctctctggat
     1801 atgtctttac atcagactca cacctctgaa acccggtttt cacacaagga gacttgccat
     1861 tggggtcttt agtagcccag ggaaacatag agtaaactga agagcttact gtgtgccgct
     1921 tatccttaga tggcctcatc cagtcacagg tctttgtcag aatctaacca ttgattcact
     1981 gaggagaccc tactgccctg aggttccact gaagcagctt ttagtgagac tagggcaaga
     2041 atataccgaa tagcttctgt tcaaggtttg gttccagaga ctctaccatt tacaggactg
     2101 gctggagtta caacaacctc ttaaagaggg tagggcgggt tctccagtta gaccccctaa
     2161 cctcacaggt gagtagtcct aacctgacag ccttagaaac acagttgccg agtacctgag
     2221 agcacccagt ggcccgccgc ctcctattca gcccctcttt aaagacgaca gacggttaag
     2281 ccatggagaa gctgttcagg tagcacgggg ccatttctgc ccccagcttg tcatgttttt
     2341 tctctcagga cccactctct gaccagtaga cttgaattta gccccaagtg atttactaaa
     2401 ctcagctcag aagctcactt aaaaatagga cctccacatg gtcgtgctaa gtaaaaatga
     2461 agaccccaag ttgcacaggg tgctgctatc tcaaagacaa tactttcctt tgaatagaaa
     2521 gctgtgccgt ccaccctgca atgggtgcta gccaacttgc ttgagaaagt caggagcaag
     2581 cattttgagt tttaaagaat gtcctgaact tgtcttttaa gtaaacatag ctgctttatt
     2641 aaaagctgtg actactaaag ccagtcacag ccatcgtcac tgtgggagtt aaatcatgtc
     2701 aactttcaaa catcacagtt gtttcaatct tctgtccaaa gaactcctct tctggagaaa
     2761 acctgatttt ggttttggct ttttgtcttg tcttgttttg tttttcctga gatggggtct
     2821 ctctgtgaag ccctggctat cctggaactc agtgtgtaga ccaggctggc cttgaactca
     2881 tgtagatctg cctgctaaaa ctaaagacat gcaccaccat gacagctgac tattttttat
     2941 gatgagcaca gaagtggctc acattttctt aaagcacact tttgtcacat agtagagtct
     3001 aggaaagaat aacattgaaa atccccatcc cagtgtgaca cttgtgtaag cacatatgga
     3061 tagccattgg aagaatacat gcttgcttgt aacatgaaat taaagacatt gtataatttc
     3121 ttaatcttta aaaattacca aaaaagtggc aacagtatgt ttaaggttat tttaattttt
     3181 acataaattg ttaaccatat ttgtttgcct ttaagtatat tctaagaatt ataattaagt
     3241 ataattataa gtcatcccgc acattcagca cttatggact aggtatgtca aaatacacaa
     3301 attctgtatt tcttgtttca taggaagaaa gcagccatct gaagctctac tcatatatat
     3361 gcctaagaat atctatacaa aattaaagtt taaaaggcct ttgcctctcg tgcagctgag
     3421 aatagacaac ttataagcct tatccaaaaa ccatgataaa gggatcatgt gcatccttac
     3481 aaaaacccct tatcctgtgc atacttacat ccaagtacat cctaaggaag gtgtccgtgc
     3541 attcatctgc cacagtgggg aagctgcctg gtgcaaggtg tgagtcctgg aatgattagt
     3601 gatagtggtc tcaaatgagg acaccttcgt ctcccaccag tttccttcct atggacaggc
     3661 tcagtccagt tatacaggcc aggaggggct ggtgcccaca gcctcccgcc tctgcaaggt
     3721 aggattgcat atctcaaaca tctctaactc agtttcggct ttgttggtct tgtttaagga
     3781 aataagccag taagccaaaa ctatttttgt tgttgttctc ttgtcttctg aaagagaggg
     3841 gatatcaatt ttaaatgttt ctctcaattt gatctttcaa atttttctaa taaagtgcca
     3901 aattgctgct cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg
     3961 gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct
     4021 gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cttggtggcg tgaaactccc
     4081 gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct tcgtaccccg gccatcaaca
     4141 cgcgtctgcg ttcgaccagg ctgcgcgttc tcgcggccat agcaaccgac gtacggcgtt
     4201 gcgccctcgc cggcagcaag aagccacgga agtccgcccg gagcagaaaa tgcccacgct
     4261 actgcgggtt tatatagacg gtccccacgg gatggggaaa accaccacca cgcaactgct
     4321 ggtggccctg ggttcgcgcg acgatatcgt ctacgtaccc gagccgatga cttactggcg
     4381 ggtgctgggg gcttccgaga caatcgcgaa catctacacc acacaacacc gcctcgacca
     4441 gggtgagata tcggccgggg acgcggcggt ggtaatgaca agcgcccaga taacaatggg
     4501 catgccttat gccgtgaccg acgccgttct ggctcctcat atcggggggg aggctgggag
     4561 ctcacatgcc ccgcccccgg ccctcaccct catcttcgac cgccatccca tcgccgccct
     4621 cctgtgctac ccggccgcgc ggtaccttat gggcagcatg accccccagg ccgtgctggc
     4681 gttcgtggcc ctcatcccgc cgaccttgcc cggcaccaac atcgtgcttg gggcccttcc
     4741 ggaggacaga cacatcgacc gcctggccaa acgccagcgc cccggcgagc ggctggacct
     4801 ggctatgctg gctgcgattc gccgcgttta cgggctactt gccaatacgg tgcggtatct
     4861 gcagtgcggc gggtcgtggc gggaggactg gggacagctt tcggggacgg ccgtgccgcc
     4921 ccagggtgcc gagccccaga gcaacgcggg cccacgaccc catatcgggg acacgttatt
     4981 taccctgttt cgggcccccg agttgctggc ccccaacggc gacctgtata acgtgtttgc
     5041 ctgggccttg gacgtcttgg ccaaacgcct ccgttccatg cacgtcttta tcctggatta
     5101 cgaccaatcg cccgccggct gccgggacgc cctgctgcaa cttacctccg ggatggtcca
     5161 gacccacgtc accacccccg gctccatacc gacgatatgc gacctggcgc gcacgtttgc
     5221 ccgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     5281 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
     5341 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5401 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5461 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
     5521 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
     5581 cgtggggtca ggtccacgac ccaagcttgg ctgcaggtcg agctcgcgaa agcttggcac
     5641 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5701 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5761 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     5821 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     5881 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     5941 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6001 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     6061 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6241 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6301 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6361 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6421 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     6481 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6541 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6601 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6661 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6721 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     6781 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6841 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6901 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6961 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7021 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7081 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt
     7261 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
     7321 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
     7381 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
     7441 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
     7501 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
     7561 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
     7621 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     7681 agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7741 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg
     7801 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc
     7861 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt
     7921 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc
     7981 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa
     8041 catagtaagc cagtatacac tccgctagca acctggctta tcgaaatatt taaatggagg
     8101 ccatcatctg ctctccattt cttatgcctg gtgggattcc tagccacctc cagcacccag
     8161 tctctggacc tgtggcatta cgtgtactgc cacaaagcca atgcagtagg acagaaggga
     8221 ctttttgctt gttccgtggg aaagcctgct tgacttttgg ccaaggcctg gcactcccac
     8281 atagctaaca gaagagcagc tggaaagcct tgtatgtttg gtgcctttag attatactgg
     8341 ctttgtttca tttctctgaa cagtgctctc aacgggaaaa ggacaatgct gcaccctagt
     8401 gtcactgcta acccacttca ttatttccac cacagccatt ttacagtctg tgccctgtag
     8461 ttcttccccg tgtaaacagt gaacggttac agattctatc caaccgtcca aatagagaat
     8521 tatgatgcag gccttatcga atgaacctgt catgatttca ttatgctcat taagggccat
     8581 gacagtcctt attggacagg aagctgattt tttttttcct aaggaaagtc atagtctcag
     8641 actcttaaca aggtaagctg tagacacatt tctactttca gagagcattg agtgcttcag
     8701 aaggaaagca gttaggccat gacatttaga gcgttttcta agaccagtat catagttaag
     8761 tggaggtata aaaaaaaaga tgctgaacat ggagtgagga gaaccgaggc tgtccgcaca
     8821 gtgtgtgacc tgggcaggca ttctccttcc tcattttgtc ttccatccct acagtgaggt
     8881 ggcacacctg acctatgtgt ctctcacagc tttttacagg agatggagaa gacaacaggc
     8941 tctaagtttt attcgtatgt atttgtgtat gtgtgtatgt gtgtgatatg tatccatgca
     9001 ttaggtgtgg ttaatatgta tttacatatc agactggcag acagactgtt tccctgaaag
     9061 cgtctggctt ctgactgctg tattcataag gtttcaaatc agcaagtatg ttcaaagcta
     9121 ctaataacac tcaggaggct gagacaggag gattttcagt tggagaccag cctgggatac
     9181 ctagtgaaac actatctcaa aagaaaagat gctgtctgtc aataaagtga tttaaagtct
     9241 aagcatgatt acaatccgtt cctaaggggc tgataatgag gatgtactaa agtccttgta
     9301 caaaagggca cacatttcaa actgtttgtc agccatatcc catactaccc aaacccatcc
     9361 ccatgttact tttcgtgatt atgacaacat ttttacccct tcattaccca ctatagacct
     9421 ggctattgag cacacttata tgatgcccaa gtcatgattt tgctcaatga ttccaggaag
     9481 tctatccatg agtctttgta tgcctgagta atgtaactct ctgcagatgc ccagtgggga
     9541 cagagttagt gtcttgcctc ggcacccagg ggcatgtgcg tgggtgatgg cgaagctgct
     9601 ctttccaggg aggtctgtga cctgggctct gtaaagggaa gggaacagtg ataaagccct
     9661 gtgacattta cctgtcacat tatattatcc tgtgacattt accacctcct ttcctaaaag
     9721 atgcttgtga ccacgctttc taggcctgcg cgtcaggaag tcagaacaga accttaattc
     9781 aggccagcgc catttctagt tagggttaaa atagtttgga tcattatcta ggtcaccata
     9841 tatcctgtcc gctcaactgt gattttgtct tcttttagtt cagagttact gagtctgtac
     9901 cgtccaaata cacttagcac ctatacataa actataaata atactacacc tggataaatc
     9961 atatagggcc acctgcttac cattaataag taacaaatgg tactgtaatg tgcagtttca
    10021 gtaaattacc gccaaccctc attgtaacca taaattattg ttgtcacgta cataaagaca
    10081 ccagggctca tagttgtcat gtaatttgtc cttacctgtc aattaagggc aggtgctgaa
    10141 tttgaagcca cgtccgccta tttccaaagc tcttttatga ctcgctgttt cctccacatt
    10201 tctgatccca gaatggtagt caggggggaa tctaaatgat accctttcta attttctgtt
    10261 tctcactctg gaataccatt acacagcgct tccataagac aggcccatcg tagtttttaa
    10321 ccttatcttt gagtctaatt tatgtaatgg tttagaatat tgctctttaa tttacgaacc
    10381 tcagccaaag tgatgcgtgt cacaagccgt gccggacgag ctgcctgaaa acacattttc
    10441 acagaactgt tgtatcacac cacaaagcga agcttcgggt gtaggtgaag acttggtata
    10501 tctgatgagt gtccttcaga atggcagacc ctgccttgtc actacaccaa gacttacacc
    10561 aggggggcgc cctgccctgt cactaagccg ggacttctcc ctggctgcac cagtgccatt
    10621 cacatacaca ccttccatcg ggggtctgct gccagggaca gacacttcct cttgtcagtg
    10681 aagtttgcag tagggtaaat gagaagtcct ttctgccatg aggtggggtt ctccctgtgc
    10741 cgctctaaga tttggtaagg aaggtcatgt ttaaaccgca ggcatttaca ttcccgtctg
    10801 gttcttccgt agcatcattg gactcccagg agaggaagac tggcctaggg acgtggccct
    10861 tccccggcag gcttttcatt ccaaatctgc tcaacccatc gagaagtttg tgacagatat
    10921 tgacgaacta ggcaaagacc tacttctggt aagttcctgg gatgccgtca ccttgtgccc
    10981 caaccatgtg gcaacgacgt ggcgtgggag gctgtatttg tttttgtgga ttccgtttcc
    11041 agctggtgag ttttgattct gcaatgtctt cctaacccag ctctcaacac ttgaatgtga
    11101 atttggacga agctttatga gacgctaggt tccttgggca aaggcttctt gacaagaaat
    11161 tgcttttctt cgcttgaagt ttcatgccac ttgaagcaac gtgctagtcg catctttgca
    11221 aaatatttcc ccccaaagga accaagtaac catacttgta gttattgatt tactgaataa
    11281 gtaccttgtg tgctattcac atataagata tcccattaaa aacaagatcc tagggctgga
    11341 gaggtgtctc cgttctcaag aatatgtatc atttttgcag agggcccaag ttcagttctc
    11401 agcacctgta cccagcagct tacagcagcc tggaactcta gctccagaga cactcttggc
    11461 cccacaggcg cttgggttca aacgcacatt cccacacaca gagacgcgaa tacttattct
    11521 taaaatgaag tttttttttt tttaataagc acttttgtat tttgaaactc aaactcctcg
    11581 ttgttggtgt tgtgtataat gtccttcctg cagacacaga aggccctctt ccttctgttc
    11641 tcagagtcag agccctatga agaataagtc tgttattgat cctgaattag gagcccgtac
    11701 tcttgattca gaccgtgggt ctatcaaacc tgcatgtgtc tgtgtgtctc accctgcctg
    11761 gtggcatgtt tttctcatgg aggcaagtag aaccatgacc aaaggcctag gtcagttatg
    11821 tggtcagggc agcttgaaga aaggtgagaa tgttcaaccc ttgagtaagt tgtgatacta
    11881 gaaggtaatt ttaagtcagg tgtctggtaa tcacattaat tgtttttacg gaagcagcac
    11941 ttcttgtgtc ggggtgtttt actttagttc atgtcgtgct ggtgactttt ctccccaaat
    12001 agaactgtgg aaaaatttta gccacctttt ccaagacttc ccccaaggtc cagggttggc
    12061 cgtgctatgt ttcacaccag atgttctctg tattgcagaa atgcctgacg tttaatccag
    12121 ctaaaaggat atccgcctac ggcgccctga atcacccgta cttccaagat ctggagagat
    12181 acaaggacaa cctgaactct cacctgccat ccaaccagag cacctcggag ctgaacacag
    12241 ccggaggatc tggcggaaga tctgaaggcc gcggcagcct gctgacatgc ggcgacgtgg
    12301 aagagaaccc tggccctgct cctggcagca caatggtgtc taagggcgaa gagctgatta
    12361 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
    12421 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
    12481 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
    12541 gcagaacctt catcaaccac acccagggca tccccgattt ctttaagcag tccttccctg
    12601 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
    12661 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
    12721 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc aacaccgaga
    12781 tgctgtaccc cgctgacggc ggcctggaag gcagaaccga catggccctg aagctcgtgg
    12841 gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga
    12901 acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc aaggaggccg
    12961 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
    13021 gcaaactggg gcacaaactt aatggcatgg acgagctgta caagggagga tctggcggcc
    13081 gctctgccac aaacttcagc ctgctgaagc aggccggcga cgccgctgaa aatcctggcc
    13141 ctgctcctgg aagcaccatg ggtgctagcg agctgatcat ctctggctcc tctggaggat
    13201 tcctgaggaa catcggcaag gagtaccagg aggctgctga gaacttcatg agattcatga
    13261 atgaccaggg agcctacgcc cctaacaccc tgagagacct gaggctggtg ttccactcct
    13321 gggctagatg gtgccacgct agacagctgg cctggttccc tatctctcct gagatggcta
    13381 gggagtactt ccttcagctg cacgatgctg acctggcctc taccaccatc gacaagcact
    13441 acgccatgct gaacatgctg ctgtcccact gtggcctgcc tcctctgtct gatgacaagt
    13501 ctgtgagcct ggccatgagg agaatccgga gagaggctgc caccgagaag ggagagagaa
    13561 ccggccaggc catccctctg agatgggatg acctgaagct gctggatgtg ctgctgtcta
    13621 gatctgagag actggtggac ctgaggaata gggccttcct gtttgtggcc tacaacaccc
    13681 tgatgaggat gtctgagatc tctaggatca gagtgggaga cctggaccag accggagaca
    13741 ccgtgaccct gcacatctcc cacaccaaga ccatcaccac cgctgctggc ctggacaaag
    13801 tgctgtctag gaggaccacc gctgtgctga atgactggct ggatgtgtct ggcctgagag
    13861 agcaccctga cgctgtgctg ttccctccta tccaccggag caacaaggct aggatcacca
    13921 ccacccctct gaccgcccct gccatggaga agatttttag cgatgcctgg gtgctgctga
    13981 acaagaggga tgccacccct aacaagggcc gctaccggac ctggaccggc cactctgcta
    14041 gagtgggagc tgccatcgac atggctgaga agcaagtgtc catggtggag atcatgcagg
    14101 agggcacctg gaaaaagcct gagacactga tgagatacct gaggagggga ggagtgtctg
    14161 tgggagccaa ctctaggctg atggactccg ctagcggcgc cctggatgct gttgctctcc
    14221 cacagccagt gggcgttcca aatgaaagcc aagccctaag ccagagattc actttttcac
    14281 caggtcaaga catacagttg attccaccac tgatcaacct gttaatgagc attgaaccag
    14341 atgtgatcta tgcaggacat gacaacacaa aacctgacac ctccagttct ttgctgacaa
    14401 gtcttaatca actaggcgag aggcaacttc tttcagtagt caagtggtct aaatcattgc
    14461 caggttttcg aaacttacat attgatgacc agataactct cattcagtat tcttggatga
    14521 gcttaatggt gtttggtcta ggatggagat cctacaaaca cgtcagtggg cagatgctgt
    14581 attttgcacc tgatctaata ctaaatgaac agcggatgaa ggaatcatca ttctattcat
    14641 tatgccttac catgtggcag atcccacagg agtttgtcaa gcttcaagtt agccaagaag
    14701 agttcctctg tatgaaagta ttgttacttc ttaatacaat tcctttggaa gggctacgaa
    14761 gtcaaaccca gtttgaggag atgaggtcaa gctacattag agagctcatc aaggcaattg
    14821 gtttgaggca aaaaggagtt gtgtcgagct cacagcgttt ctatcaactt acaaaacttc
    14881 ttgataactt gcatgatctt gtcaaacaac ttcatctgta ctgcttgaat acatttatcc
    14941 agtcccgggc actgagtgtt gaatttccag aaatgatgtc tgaagttatt gctatgcatg
    15001 cgtacggata gggcgcgccg gatccattat gtacctgact gatgaagttc ctatactttc
    15061 tagagaatag gaacttcgaa gggttccgca agctctagtc gagccccagc tggttctttc
    15121 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
    15181 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
    15241 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
    15301 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga
    15361 ggctgatcag cgagctctag agaattgatc ccctcagaag aactcgtcaa gaaggcgata
    15421 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
    15481 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
    15541 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
    15601 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcatcgccgt cgggcatgcg
    15661 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
    15721 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
    15781 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
    15841 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
    15901 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
    15961 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
    16021 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
    16081 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
    16141 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatggccg atcccatggt
    16201 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
    16261 aacaggctgc aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg
    16321 cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc
    16381 ctgagcccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa
    16441 gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt ccattgctca
    16501 gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg tcacgtcctg
    16561 cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc
    16621 gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt ggaatgtgtg
    16681 cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct
    16741 ccagactgcc ttgggaaaag cgcctcccct acccggtaga atttcgacga cctgcagcca
    16801 agctagcttg gctggacgta aactcctctt cagacctgaa gttcctatac tttctagaga
    16861 ataggaactt cggaattcga tatcaagctt atcgataccg gttccacggg gatgcccatg
    16921 agctcgtcat ctgaacacat tggcggctgc gagtccccta agcaagcctc tcagagcagt
    16981 tgaagattgc tggctgccaa ccttctggct gccagcttct gggtgggctc tgccttacca
    17041 aggaaaccac ctagtttact gttcagagat caatgcaagg gtgattgcag ctttatgttc
    17101 gtttgtacac ttgtttgttt tgtctgtttg tttcaagaac ctggaaaact tccagaagaa
    17161 gagaagctgc tgaccaattg tgctgccatt tcgttttcta accttgaatg ctgccagtgt
    17221 agggtgggaa tccaggccca gctgagttat gatgtaatcc gcctgcagct gctgggcctg
    17281 ctttggtact tgtgagtgtg tgtgcatgcg tatgtgtgtg taagagagaa gaggagggga
    17341 gagaaagacc cctgatctcg tcaagtgtta cttttttttt gtagaaaaca agaataattg
    17401 agttttaaag agtagaggtg actgatagta agaagggctt gttcagtgaa aggtgattca
    17461 caatggagtc ttgttaggaa ggttggacct aagtcctcag agttgccttc ctgtccaaaa
    17521 gcttttgcta gcagtaaaca ataaaggttt agatgccaca aaaaatgggg ggaaccacaa
    17581 tattttttaa gagacttttt aaggcataca tcttctattt actctttgga aagctgaact
    17641 taatgtgtcc caggccctat atatagtaca gtatgtactt aattgtttct ttggggaaag
    17701 atgctataag tatcttatta cttgcaatac atttaaggag tgagtgtacc tcagataggt
    17761 tttaaagata gagagcacct gttttctggt gtgagatgtt atcattttct tcacgtctct
    17821 tgataccttg ataccttgtc accttaggga atcacttcct gctctgacta gaggcgggaa
    17881 taccatctag ctgtctccac cacccaccat ggcgcatctg ccttgtgctg ccttgtgtag
    17941 tgcgaagctc tcaaccacca gcacttctaa ttcattttcc tgccactgcc tggctaacga
    18001 cagatggccc agctgcccca atcccacacc cgcttgcacg cttaccgtct ttcaccgaat
    18061 gctttgggcg taggctccca ttccgaaacc ctaacagtat ccccttgtgc ctttgtaata
    18121 cagtcttccc cctgccgcag ctgaggtcac ctaggcagtg aagagtgctt gt
//
