LOCUS       sequence-31510-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARL5:Q15-L176 (2H17)
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             608..625
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(678..696)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      692..739
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtcaggagca caaagttatc attgttgggc
      121 tggataatgc agggaaaact accattcttt accaattttc tatgaatgaa gttgtacata
      181 catctcctac aataggaagt aatgtagaag agatagtgat taataataca cgtttcctaa
      241 tgtgggatat tggtggccaa gaatctcttc gttcttcctg gaacacttac tatactaaca
      301 cagagtttgt aatagttgtt gtggacagta cagacagaga gaggatttct gtaactagag
      361 aagaactcta taaaatgtta gcgcatgagg acctaagaaa agctggattg ctgatttttg
      421 ctaataaaca agatgttaaa gaatacatga ctgtagcaga aatctcccag tttttgaagc
      481 taacttctat taaagatcac cagtggcata tccaggcatg ctgtgctcta actggcgagg
      541 gattgtgcca aggacttgaa tggatgatgt cacgactttg atgacgaagc ttgcggccgc
      601 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      661 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      721 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      781 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      841 cttgccagcg ccctagcgcc cgctcctttc gc
//
