LOCUS       sequence-315103-        9766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dCas9 driven by lux cassette from Vibrio fischeri.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Liu Y, Chen J, Crisante D, Jaramillo Lopez JM, Mahadevan R
  TITLE     Dynamic Cell Programming with Quorum Sensing-Controlled CRISPRi 
            Circuit.
  JOURNAL   ACS Synth Biol. 2020 Jun 5. doi: 10.1021/acssynbio.0c00148.
  PUBMED    32485106
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Jun 5. doi: 10.1021/acssynbio.0c00148."
COMMENT     Sequence Label: pQdCas9-sgempty
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             85..879
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(139..158)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     749..768
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     RBS             2617..2628
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2635..2661,2662..2664,2665..5151,5152..5154,
                     5155..6741)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        6748..6782
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        6783..6858
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(6937..7599)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGE*QLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7600..8371
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atttgatcaa
       61 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      121 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      181 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      241 ctgtccggtg ccctgaatga actacaggac gaggcagcgc ggctatcgtg gctggccacg
      301 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      361 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      421 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      481 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      541 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      601 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
      661 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
      721 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
      781 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
      841 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
      901 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
      961 atgaaaggtt ggactagaga attcatcctg aacttatcta gattaattta agactgcttt
     1021 tttaaactgt tcattaatag gcatagacaa tacaaccgat ttagtatcac ctaatacatg
     1081 aatttctttg tctccaatac gatgacaagg aactttaata cgctttaaaa atcgctctat
     1141 tgctgttgat gttactgtta catattctgt aataccttga ctaacagcgt gtttatatat
     1201 agcttcaaat agtttcattg taatttcact agcagagtta tttatctttg agctattttt
     1261 acctacagca aaacgactta attcgactat attaggatct ttgggagcac tctgttgacc
     1321 aagcaattca ggaaaaacac ttttcagcat ataatcacct gttgtaggta ataaacgcca
     1381 gcatccactt acattttcag tatcatcaca agcataaata tattctgcat ttgagttatc
     1441 atactcatct gattcaaggt tattttctac aactaagtcc cactcaagtc tttgcttaaa
     1501 cacttgataa cgaagactta gaataccttt atactcctcc gatggaattg ccaaaaaatc
     1561 cgattttttt atcattatag tcataccaac ctcccttgcg tttattcgac tataacaaac
     1621 cattttcttg cgtaaacctg tacgatccta caggtgctta tgttaagtaa ttgtattccc
     1681 agcgatacaa tagtgtgaca aaaatccaat ttattagaat caaatgtcaa tccattaccg
     1741 ttttaatgat atataacacg caaaacttgc gacaaacaat aggtaaggat aaagagatgg
     1801 gtatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta
     1861 gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat
     1921 attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaattctag
     1981 ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc
     2041 ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca
     2101 atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca
     2161 ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac
     2221 attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat
     2281 taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa
     2341 acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct
     2401 cttgggatat ttcaaaaata ttaggctgca gtgagcgtac tgtcactttc catttaacca
     2461 atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa
     2521 caggagcaat tgattgccca tactttaaaa attaaagatc tacctgtagg atcgtacagg
     2581 tttacgcaag aaaatggttt gttatagtcg aataaaaaag aggagaaagg atctatggat
     2641 aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc ggtgatcact
     2701 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
     2761 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
     2821 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
     2881 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
     2941 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
     3001 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg
     3061 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt
     3121 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac
     3181 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac
     3241 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta
     3301 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt
     3361 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct
     3421 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt
     3481 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt
     3541 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt
     3601 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa
     3661 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat
     3721 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa
     3781 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa
     3841 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt
     3901 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa
     3961 atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt
     4021 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc
     4081 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt
     4141 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac
     4201 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt
     4261 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag
     4321 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga
     4381 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa
     4441 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca
     4501 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc
     4561 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg
     4621 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt
     4681 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg
     4741 acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa
     4801 catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa
     4861 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa
     4921 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa
     4981 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa
     5041 aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg
     5101 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tgccattgtt
     5161 ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa
     5221 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat
     5281 tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa
     5341 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt
     5401 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa
     5461 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta
     5521 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat
     5581 catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca
     5641 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt
     5701 gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc
     5761 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta
     5821 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca
     5881 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca
     5941 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt
     6001 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca
     6061 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag
     6121 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta
     6181 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt
     6241 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa
     6301 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat
     6361 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat
     6421 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta
     6481 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata
     6541 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct
     6601 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt
     6661 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg
     6721 agtcagctag gaggtgacta actcgagttg acagctagct cagtcctagg tataatacta
     6781 gtgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     6841 gtggcaccga gtcggtgctt tttttgttaa cttcaggacc tgcagtctag tatagtctgg
     6901 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
     6961 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     7021 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     7081 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgcta
     7141 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     7201 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     7261 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     7321 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     7381 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     7441 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     7501 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     7561 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     7621 gtgccgcacg gttgcggcac catgcgcaat cagctgcaac tttttggcag cgcgacaaca
     7681 attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta cgcaatgagc
     7741 tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt tgttgatttt
     7801 taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag ggcacccctg
     7861 cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc ccctccgggg
     7921 cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc
     7981 cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc ttcgactgcc
     8041 cccactcgca taggcttggg tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc
     8101 tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc
     8161 gcaggccgga ggcttttccc cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc
     8221 cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg tagccggtgg gcaatccctg
     8281 tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg gttgtccacg
     8341 ggccgagcga agcgagccag ccggtggccg ctcgcggcca tcgtccacat atccacgggc
     8401 tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtagggcgcc
     8461 gcagccgccg taggcggtca cgactttgcg aagcaaagtc tagtgagtat actcaagcat
     8521 tgagtggccc gccggaggca ccgccttgcg ctgcccccgt cgagccggtt ggacaccaaa
     8581 agggaggggc aggcatggcg gcatacgcga tcatgcgatg caagaagctg gcgaaaatgg
     8641 gcaacgtggc ggccagtctc aagcacgcct accgcgagcg cgagacgccc aacgctgacg
     8701 ccagcaggac gccagagaac gagcactggg cggccagcag caccgatgaa gcgatgggcc
     8761 gactgcgcga gttgctgcca gagaagcggc gcaaggacgc tgtgttggcg gtcgagtacg
     8821 tcatgacggc cagcccggaa tggtggaagt cggccagcca agaacagcag gcggcgttct
     8881 tcgagaaggc gcacaagtgg ctggcggaca agtacggggc ggatcgcatc gtgacggcca
     8941 gcatccaccg tgacgaaacc agcccgcaca tgaccgcgtt cgtggtgccg ctgacgcagg
     9001 acggcaggct gtcggccaag gagttcatcg gcaacaaagc gcagatgacc cgcgaccaga
     9061 ccacgtttgc ggctgctgtg gccgatctag ggctgcaacg gggcatcgag ggcagcaagg
     9121 cacgtcacac gcgcattcag gcgttctacg aggccctgga gcggccacca gtgggccacg
     9181 tcaccatcag cccgcaagcg gtcgagccac gcgcctatgc accgcaggga ttggccgaaa
     9241 agctgggaat ctcaaagcgc gttgagacgc cggaagccgt ggccgaccgg ctgacaaaag
     9301 cggttcggca ggggtatgag cctgccctac aggccgccgc aggagcgcgt gagatgcgca
     9361 agaaggccga tcaagcccaa gagacggccc gagaccttcg ggagcgcctg aagcccgttc
     9421 tggacgccct ggggccgttg aatcgggata tgcaggccaa ggccgccgcg atcatcaagg
     9481 ccgtgggcga aaagctgctg acggaacagc gggaagtcca gcgccagaaa caggcccagc
     9541 gccagcagga acgcgggcgc gcacatttcc ccgaaaagtg ccacctggga tgaatgtcag
     9601 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     9661 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     9721 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagta
//
