LOCUS       sequence-31511-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB26
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             654..671
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(724..742)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      738..785
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt ggtgtcgact tctacgacgt cgccttcaag
      121 gtcatgctgg tgggggactc gggtgtgggg aagacctgtc tgctggtgcg attcaaggat
      181 ggtgctttcc tggcggggac cttcatctcc accgtaggca ttgacttccg gaacaaagtt
      241 ctggacgtgg atggtgtgaa ggtgaagctg cagatgtggg acacagctgg tcaggagcgg
      301 ttccgcagtg ttacccatgc ctactaccgg gatgctcatg ctctgctgct gctctacgat
      361 gtcaccaaca aggcctcctt tgacaacatc caggcctggc tgaccgagat ccacgagtac
      421 gcccagcacg acgtggcgct catgctgctg gggaacaagg tggactctgc ccatgagcgt
      481 gtggtgaaga gggaggacgg ggagaagctg gccaaggagt atggactgcc cttcatggag
      541 accagcgcca agacgggcct caacgtggac ttggccttca cagccatagc aaaggagttg
      601 aagcggcgct ccatgtaggc tccctgatga cgaagcttgc ggccgcactc gagcaccacc
      661 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      721 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      781 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      841 attaagcgcg gcgggtgtgg tggttac
//
