LOCUS       sequence-31513-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARF5
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             647..664
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(717..735)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      731..778
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(902..921)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcctctgaa tattttgttt actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggttccgc gtggtagtct cttttcgcgg atcttcggga
      121 agaagcagat gcggattctc atggttggct tggatgcggc tggcaagacc acaatcctgt
      181 acaaactgaa gttgggggag attgtcacca ccatcccaac cataggcttc aatgtagaaa
      241 cagtggaata taagaacatc tgtttcacag tctgggacgt gggaggccag gacaagattc
      301 ggcctctgtg gcggcactac ttccagaaca ctcagggcct catctttgtg gtggacagta
      361 atgaccggga gcgggtccaa gaatctgctg atgaactcca gaagatgctg caggaggacg
      421 agctgcggga tgcagtgctg ctggtatttg ccaacaagca ggacatgccc aacgccatgc
      481 ccgtgagcga gctgactgac aagctggggc tacagcactt acgcagccgc acgtggtatg
      541 tccaggccac ctgtgccacc caaggcacag gtctgtacga tggtctggac tggctgtccc
      601 acgagctgtc aaagcgctga tgacgaagct tgcggccgca ctcgagcacc accaccacca
      661 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      721 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      781 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      841 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      901 gctcctttcg ctttcttccc ttcctttctc gccacgttcg c
//
