LOCUS       sequence-31514-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SARA1
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             689..706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(759..777)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      773..820
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtaatggctt cagcagtgtg ctccagttcc
      121 taggactgta caagaaatct ggaaaacttg tattcttagg tttggataat gcaggcaaaa
      181 ccactcttct tcacatgctc aaagatgaca gattgggcca acatgttcca acactacatc
      241 cgacatcaga agagctaaca attgctggaa tgacctttac aacttttgat cttggtgggc
      301 acgagcaagc acgtcgcgtt tggaaaaatt atctcccagc aattaatggg attgtctttc
      361 tggtggactg tgcagatcat tctcgcctcg tggaatccaa agttgagctt aatgctttaa
      421 tgactgatga aacaatatcc aatgtgccaa tccttatctt gggtaacaaa attgacagaa
      481 cagatgcaat cagtgaagaa aaactccgtg agatatttgg gctttatgga cagaccacag
      541 gaaaggggaa tgtgaccctg aaggagctga atgctcgccc catggaagtg ttcatgtgca
      601 gtgtgctcaa gaggcaaggt tacggcgagg gtttccgctg gctctcccag tatattgact
      661 gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      721 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      781 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      841 cggattggcg aatgggacgc gccctgtagc gggcgcatta agcgcggc
//
