LOCUS       sequence-31515-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KHK
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             502..513
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaattcctct gaataatttt gtttacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtt ccgcgtggta gtcagatcct gtgcgtgggg
      121 ctagtggtgc tggacgtcat cagcctggtg gacaagtacc ctaaggagga ctcggagata
      181 aggtgtttgt cccagagatg gcagcgcgga ggcaacgcgt ccaactcctg caccattctc
      241 tccctgctcg gagccccctg tgccttcatg ggctcaatgg ctcctggcca tgttgctgat
      301 tttgtcctgg atgacctccg ccgttattct gtggacctac gctacacagt ctttcagacc
      361 acaggctccg tccccatcgc cacggtcatc atcaacgagg ccagtggtag ccgcaccatc
      421 ctatactatg acaggagcct gccagatgtg tctgctacag actttgagaa ggttgatctg
      481 acccagttca agtggatcca cattgagggc cggaacgcat cggagcaggt gaagatgctg
      541 cagcggatag acgcacacaa caccaggcag cctccagagc agaagatccg ggtgtccgtg
      601 gaggtggaga agccacgaga ggagctcttc cagctgtttg gctacggaga cgtggtgttt
      661 gtcagcaaag atgtggccaa gcacttgggg ttccagtcag cagaggaagc cttgaggggc
      721 ttgtatggtc gtgtgaggaa aggggctgtg cttgtctgtg cctgggctga ggagggcgcc
      781 gacgccctgg gccctgatgg caaattgctc cactcggatg ctttcccgcc accccgcgtg
      841 gtggatacac tgggagctgg agacaccttc aatgcctccg tcatcttcag cctctcccag
      901 gggaggagcg tgcaggaagc actg
//
