LOCUS       sequence-31516-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARL8
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             658..675
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(728..746)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      742..789
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tatggggctg atcttcgcca aactgtggag
      121 cctcttctgt aaccaagaac acaaagtaat tatagtggga ctggataatg cagggaaaac
      181 caccattctt taccaattct taatgaatga agtggttcat acttctccaa ccataggaag
      241 caatgttgaa gaaatagttg tgaagaacac tcattttctt atgtgggata ttggtggtca
      301 ggagtctctg cgatcatcct ggaacacata ttactcaaat acagagttca tcattcttgt
      361 tgttgatagc attgacaggg aacgactagc tattacaaaa gaagaattat acagaatgtt
      421 ggctcatgag gatttacgga aggctgcagt ccttatcttt gcaaataaac aggatatgaa
      481 agggtgtatg acagcagctg aaatctcgaa atacctcacc cttagttcaa ttaaggatca
      541 tccatggcac attcaatcct gctgtgctct cacaggagaa gggttatgcc aaggtctaga
      601 gtggatgacc tcccggattg gtgtgagatg atgacgaagc ttgcggccgc actcgagcac
      661 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      721 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      781 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca c
//
