LOCUS       sequence-315169-       10629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Edit Ade2 gene in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Sankaran SM, Smith JD, Roy KR
  TITLE     CRISPR-Cas9 Gene Editing in Yeast: A Molecular Biology and 
            Bioinformatics Laboratory Module for Undergraduate and High School 
            Students.
  JOURNAL   J Microbiol Biol Educ. 2021 May 31;22(2):e00106-21. doi: 
            10.1128/jmbe.00106-21. eCollection 2021 Fall.
  PUBMED    34594460
REFERENCE   2  (bases 1 to 10629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/jmbe.00106-21"; journalName: "J Microbiol Biol Educ"; date:
            "2021-05-31- 31"; volume: "22"; issue: "2"; pages: "e00106-21"
COMMENT     Sequence Label: pG1
FEATURES             Location/Qualifiers
     source          1..10629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3047..3064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3181..3202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3217..3222,3223..3240,3241..3247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3255..3271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3260..3282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3279..3295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3279..3295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3314..3334
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3316..3334
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3352..3750
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             3773..3793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3803..5521
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6804..6815
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8333..8353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8369..8616
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8385..8403)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(8634..8653)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8635..8653)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8663..8680)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8663..8679)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8672..8694)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8824..9279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8911..8930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9121..9142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9410..10213)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10214..10434)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10460..10479)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10579..10601
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctct taattaagta
     2401 tatgtgttat gtagtatact ctttcttcaa caattaaata ctctcggtag ccaagttggt
     2461 ttaaggcgca agactgtaat ttatcactac gaaatcttga gatcgggcgt tcgactcgcc
     2521 cccgggagag atggccggca tggtcccagc ctcctcgctg gcgccggctg ggcaacacct
     2581 tcgggtggcg aatgggactt tggcggttgt ggtatatttg gtggtttaag agctatgctg
     2641 gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     2701 agtcggtgct tttttgttaa ctccgttact gtgggcttgc catatttttt ggaacttttc
     2761 tgcccttttt ctcaatgagt aaggagggcg tttatctttt ctattttatt tttctacaaa
     2821 tttgattcat gagtccatgg aacaaatata tagaactcat tttcttgttt cggtatatat
     2881 attaactcca ttgaagcatt tgcttttatc ctgactggct agctagtcgt gcaatgtatg
     2941 actttaagcc gcggtctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3001 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3061 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     3301 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcatagcttc
     3361 aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta
     3421 ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt
     3481 cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg tttctttttc
     3541 ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt
     3601 tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcttcaat
     3661 ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat
     3721 tagaaagaaa gcatagcaat ctaatctaag ttttctagag atgtctagag ccccaaagaa
     3781 gaagagaaaa gttagacccg ggatggacaa gaagtactcc attgggctcg atatcggcac
     3841 aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa
     3901 agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt
     3961 cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac
     4021 ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt
     4081 ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca
     4141 cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc
     4201 aaccatatat catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt
     4261 gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga
     4321 cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa
     4381 tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag
     4441 cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa
     4501 gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa
     4561 atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga
     4621 tgatctcgac aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc
     4681 aaagaacctg tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac
     4741 caaagctccg ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac
     4801 tttgctgaag gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga
     4861 tcagtctaaa aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta
     4921 caaatttatt aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct
     4981 taacagagaa gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca
     5041 gattcacctg ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt
     5101 gaaagataac agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg
     5161 ccccctcgcc cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat
     5221 cactccctgg aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga
     5281 aaggatgact aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct
     5341 gctgtacgag tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg
     5401 gatgagaaag ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt
     5461 caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga
     5521 atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac
     5581 gtatcacgat ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga
     5641 ggacattctt gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga
     5701 agaacgcttg aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag
     5761 gcgccgatat acaggatggg ggcggctgtc aagaaaactg atcaatgggg tatgtgaact
     5821 gcaatattaa tagcacgaga aaattgagag gaagatagat gggaactagt agagttgata
     5881 ttgatgagat acgaaaacca cacgtaaata aactatccga cgacaagaat agtgcattaa
     5941 ggacttgatt aagatatggt gagcaacgta attatcgggc tcaacagttt attagcaatc
     6001 gttttgatag aagcgttgat gctgtggaag ttgttctttt tactaccaac agccattaac
     6061 aaatccatta gagtgttcgt tcgtttttct gctcagattc aagaaaatta ttccatcctc
     6121 attatacttt tttcttctat ttcgtgctcc acgtcgaggt atcaaggaac atagtttact
     6181 aacattaacg aattcatctc ctatgaattt actttttgta tagatccgag acaagcagag
     6241 tggaaagaca atcctggatt ttcttaagtc cgatggattt gccaaccgga acttcatgca
     6301 gttgatccat gatgactctc tcacctttaa ggaggacatc cagaaagcac aagtttctgg
     6361 ccagggggac agtcttcacg agcacatcgc taatcttgca ggtagcccag ctatcaaaaa
     6421 gggaatactg cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa
     6481 gcccgagaat atcgttatcg agatggcccg agagaaccaa actacccaga agggacagaa
     6541 gaacagtagg gaaaggatga agaggattga agagggtata aaagaactgg ggtcccaaat
     6601 ccttaaggaa cacccagttg aaaacaccca gcttcagaat gagaagctct acctgtacta
     6661 cctgcagaac ggcagggaca tgtacgtgga tcaggaactg gacatcaatc ggctctccga
     6721 ctacgacgtg gatcatatcg tgccccagtc ttttctcaaa gatgattcta ttgataataa
     6781 agtgttgaca agatccgata aaaatagagg gaagagtgat aacgtcccct cagaagaagt
     6841 tgtcaagaaa atgaaaaatt attggcggca gctgctgaac gccaaactga tcacacaacg
     6901 gaagttcgat aatctgacta aggctgaacg aggtggcctg tctgagttgg ataaagccgg
     6961 cttcatcaaa aggcagcttg ttgagacacg ccagatcacc aagcacgtgg cccaaattct
     7021 cgattcacgc atgaacacca agtacgatga aaatgacaaa ctgattcgag aggtgaaagt
     7081 tattactctg aagtctaagc tggtctcaga tttcagaaag gactttcagt tttataaggt
     7141 gagagagatc aacaattacc accatgcgca tgatgcctac ctgaatgcag tggtaggcac
     7201 tgcacttatc aaaaaatatc ccaagcttga atctgaattt gtttacggag actataaagt
     7261 gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa
     7321 gtacttcttt tacagcaata ttatgaattt tttcaagacc gagattacac tggccaatgg
     7381 agagattcgg aagcgaccac ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga
     7441 caagggtagg gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt
     7501 taaaaagacc gaagtacaga ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa
     7561 cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg gcggattcga
     7621 ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa
     7681 aaaactcaaa agcgtcaagg aactgctggg catcacaatc atggagcgat caagcttcga
     7741 aaaaaacccc atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat
     7801 cattaagctt cccaagtact ctctctttga gcttgaaaac ggccggaaac gaatgctcgc
     7861 tagtgcgggc gagctgcaga aaggtaacga gctggcactg ccctctaaat acgttaattt
     7921 cttgtatctg gccagccact atgaaaagct caaagggtct cccgaagata atgagcagaa
     7981 gcagctgttc gtggaacaac acaaacacta ccttgatgag atcatcgagc aaataagcga
     8041 attctccaaa agagtgatcc tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa
     8101 taagcacagg gataagccca tcagggagca ggcagaaaac attatccact tgtttactct
     8161 gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac accaccatag acagaaagcg
     8221 gtacacctct acaaaggagg tcctggacgc cacactgatt catcagtcaa ttacggggct
     8281 ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg accccaagaa
     8341 gaagaggaag gtgtgatctc ttctcgagtc atgtaattag ttatgtcacg cttacattca
     8401 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     8461 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
     8521 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     8581 gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat tcgccctata
     8641 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8701 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8761 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     8821 gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8881 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8941 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9001 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9061 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9121 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9181 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9241 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ctgatgcggt attttctcct
     9301 tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag
     9361 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
     9421 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
     9481 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
     9541 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
     9601 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
     9661 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
     9721 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
     9781 taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
     9841 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
     9901 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     9961 tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
    10021 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
    10081 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
    10141 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
    10201 tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta
    10261 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
    10321 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
    10381 aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagctg
    10441 tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
    10501 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    10561 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
    10621 aacgcgcga
//
