LOCUS       sequence-31517-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB2B
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             645..662
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(715..733)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      729..776
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(900..919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcctctga atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggttccg cgtggtagtt atctcttcaa gtatatcatc
      121 atcggagaca caggtgtggg gaagtcatgt ctcctcctgc agtttacaga taagcggttc
      181 cagcctgtcc acgacctcac aataggtgtg gagtttggag ctcgtatggt caacattgat
      241 ggaaaacaaa tcaaactgca aatctgggat acggctgggc aagaatcctt ccgttctatc
      301 acccgttcct actacagggg agcagctgga gcactgctgg tgtacgacat tacaaggcgt
      361 gaaaccttca accacctgac ctcatggtta gaggatgccc ggcagcactc tagttccaac
      421 atggttatca tgctcattgg gaataagagt gacctagagt cccgcaggga tgtgaagaga
      481 gaagaaggag aggcctttgc tagggagcat ggacttatat tcatggaaac ttcagccaaa
      541 acagcctgca atgttgaaga ggccttcatt aacacagcca aagaaatata taggaagatc
      601 cagcagggtt tattttgatg acgaagcttg cggccgcact cgagcaccac caccaccacc
      661 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      721 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      781 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      841 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      901 tcctttcgct ttcttccctt cctttctcgc cacg
//
