LOCUS       sequence-315175-       10616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty control for Cas9 experiments and to clone new guide RNAs for 
            paper.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Sankaran SM, Smith JD, Roy KR
  TITLE     CRISPR-Cas9 Gene Editing in Yeast: A Molecular Biology and 
            Bioinformatics Laboratory Module for Undergraduate and High School 
            Students.
  JOURNAL   J Microbiol Biol Educ. 2021 May 31;22(2):e00106-21. doi: 
            10.1128/jmbe.00106-21. eCollection 2021 Fall.
  PUBMED    34594460
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/jmbe.00106-21"; journalName: "J Microbiol Biol Educ"; date:
            "2021-05-31- 31"; volume: "22"; issue: "2"; pages: "e00106-21"
COMMENT     Sequence Label: pEmpty_Control
FEATURES             Location/Qualifiers
     source          1..10616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3034..3051
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3168..3189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3204..3209,3210..3227,3228..3234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3242..3258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3247..3269
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3266..3282
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3266..3282
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3301..3321
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3303..3321
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3339..3737
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             3760..3780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3790..5508
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6791..6802
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8320..8340
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8356..8603
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8372..8390)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(8621..8640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8622..8640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8650..8667)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8650..8666)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8659..8681)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8811..9266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8898..8917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9108..9129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9397..10200)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10201..10421)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10447..10466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10566..10588
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctct taattaagta
     2401 tatgtgttat gtagtatact ctttcttcaa caattaaata ctctcggtag ccaagttggt
     2461 ttaaggcgca agactgtaat ttatcactac gaaatcttga gatcgggcgt tcgactcgcc
     2521 cccgggagag atggccggca tggtcccagc ctcctcgctg gcgccggctg ggcaacacct
     2581 tcgggtggcg aatgggactt tggcggccgc gtttaagagc tatgctggaa acagcatagc
     2641 aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     2701 ttgttaactc cgttactgtg ggcttgccat attttttgga acttttctgc cctttttctc
     2761 aatgagtaag gagggcgttt atcttttcta ttttattttt ctacaaattt gattcatgag
     2821 tccatggaac aaatatatag aactcatttt cttgtttcgg tatatatatt aactccattg
     2881 aagcatttgc ttttatcctg actggctagc tagtcgtgca atgtatgact ttaagccgcg
     2941 gtctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3001 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3061 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3121 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     3181 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     3241 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     3301 gcaattaacc ctcactaaag ggaacaaaag ctggagctca tagcttcaaa atgtttctac
     3361 tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca
     3421 cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt taattacccg
     3481 tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag
     3541 gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga tttttttctc
     3601 tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc tcaagtttca
     3661 gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag aaagaaagca
     3721 tagcaatcta atctaagttt tctagagatg tctagagccc caaagaagaa gagaaaagtt
     3781 agacccggga tggacaagaa gtactccatt gggctcgata tcggcacaaa cagcgtcggc
     3841 tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat
     3901 accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag
     3961 acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat
     4021 cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc
     4081 ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca
     4141 atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat
     4201 ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg
     4261 ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac
     4321 aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa
     4381 gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc
     4441 aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg
     4501 tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac
     4561 ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat
     4621 ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa gaacctgtca
     4681 gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa agctccgctg
     4741 agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt gctgaaggcc
     4801 cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca gtctaaaaat
     4861 ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa atttattaag
     4921 cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa cagagaagat
     4981 ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat tcacctgggc
     5041 gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa agataacagg
     5101 gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc cctcgcccgg
     5161 ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agaccatcac tccctggaac
     5221 ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag gatgactaac
     5281 tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct gtacgagtac
     5341 ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat gagaaagcca
     5401 gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg
     5461 aaagttaccg tgaaacagct caaagaagac tatttcaaaa agattgaatg tttcgactct
     5521 gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta tcacgatctc
     5581 ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga cattcttgag
     5641 gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga acgcttgaaa
     5701 acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg ccgatataca
     5761 ggatgggggc ggctgtcaag aaaactgatc aatggggtat gtgaactgca atattaatag
     5821 cacgagaaaa ttgagaggaa gatagatggg aactagtaga gttgatattg atgagatacg
     5881 aaaaccacac gtaaataaac tatccgacga caagaatagt gcattaagga cttgattaag
     5941 atatggtgag caacgtaatt atcgggctca acagtttatt agcaatcgtt ttgatagaag
     6001 cgttgatgct gtggaagttg ttctttttac taccaacagc cattaacaaa tccattagag
     6061 tgttcgttcg tttttctgct cagattcaag aaaattattc catcctcatt atactttttt
     6121 cttctatttc gtgctccacg tcgaggtatc aaggaacata gtttactaac attaacgaat
     6181 tcatctccta tgaatttact ttttgtatag atccgagaca agcagagtgg aaagacaatc
     6241 ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat
     6301 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
     6361 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
     6421 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
     6481 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa
     6541 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
     6601 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
     6661 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat
     6721 catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
     6781 tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
     6841 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
     6901 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg
     6961 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
     7021 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
     7081 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
     7141 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
     7201 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
     7261 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
     7321 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
     7381 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
     7441 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
     7501 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
     7561 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
     7621 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc
     7681 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
     7741 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
     7801 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag
     7861 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
     7921 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
     7981 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
     8041 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
     8101 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc
     8161 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca
     8221 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
     8281 atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa gaggaaggtg
     8341 tgatctcttc tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca
     8401 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     8461 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     8521 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     8581 gacgctcgaa ggctttaatt tgcggccggt acccaattcg ccctatagtg agtcgtatta
     8641 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8701 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8761 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg
     8821 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8881 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8941 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9001 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9061 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9121 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9181 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     9241 taacaaaata ttaacgttta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     9301 ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc taatttgtga
     9361 gtttagtata catgcattta cttataatac agttttttag ttttgctggc cgcatcttct
     9421 caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc
     9481 ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc
     9541 cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt
     9601 cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc
     9661 gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt
     9721 agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt
     9781 tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc
     9841 attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac
     9901 tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga
     9961 taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg
    10021 tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt
    10081 ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag
    10141 cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat
    10201 gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca
    10261 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt
    10321 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa
    10381 tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagctgtgg tatggtgcac
    10441 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
    10501 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
    10561 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
