LOCUS       sequence-315177-       11341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10TC)_Psyn-sgRNA500
FEATURES             Location/Qualifiers
     source          1..11341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1452..1486
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        2003..2078
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(3521..3535,3536..3541,3542..3558,3559..3565)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3541..3557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3573..3589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3608..3619
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(3626..3652,3653..3655,3656..6142,6143..6145,
                     6146..7732)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7902..8693
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8816..9404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9305..9324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctttcaatt gacttctcta atttacgctt tatattgttt aaatcgctat atttatgact
       61 ttcaataata aaatgaggct taaatttgta attttcgtat attttttgga aacttgtttc
      121 taattgttta gaattatatt catttttttc aaattctttt tgagtgttgc atagaatttc
      181 ctttaatttt tcttgttttt ctttcataca agatttagta aaatgtatat ttttattttt
      241 tagtaattta atttctatta tttttatgat tttaagtatt ttgattatat tatctttatc
      301 aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga atttttttac ataaaaagtt
      361 acatttattg aagtaatttc ttacttgata cttttctatt tcattaatct ttctttcttc
      421 ttttatatta tttttattat ttaaacactc ctttaaatct acactcccat ttttagtaaa
      481 tctatcttta aggccccttg aaactctatt ttgaaatctt aaatcttttt tttcttgaaa
      541 gtatttatag attttttggt aacattcttt ttttgaaaaa ttaagttgat agtatatttc
      601 ggtccccaaa tttaccccta agtgtttgtg ataattagtt gtgactttaa attctttttc
      661 taatttgtaa aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac tatttctttt
      721 aaggttttca ttaaagtaat agagtatgtt tttttgtgtg tatttcttat atgttttatt
      781 tatgtacgct agtgttgaga taaggactac taatttatac tgtaatttat tgtagcaagt
      841 tgtggtttta ctggttttgt ttatattaga taagttttcc atttatagct ccttgaatac
      901 cggatttttt atctataaag tatatatatc acaattttga tttaaaagta aattcttttt
      961 ttaaaaatac aaaaattttt tataaattat atagaccatg atattgtatt aatactaatt
     1021 gattttaatt taaggttgaa ctaaaataaa catagttttg tagaaaataa tttttcatta
     1081 ttttgctact tgatattgga tcgtaaaaat attagggctt tacaaagttc ttttaaaaga
     1141 gaatttagta aagccctaag tcttttaata aaaaattttt tgtaaaaaag ttggcaaaaa
     1201 tagtttttgc tatatattta tatataagaa aaaattcaat actcaaggag ataaaaaatg
     1261 gaaaacttaa acaataatcc acaaaaaatt aatcaaataa ataagcaaac taattttatt
     1321 ggttttgaaa attttataga acttgataat caagttaatc aaactaaaga aaaaagtaaa
     1381 ttaagtaaga taggtaagaa attacccgga ataagcagtc aagtctagtg cggccggaat
     1441 tctaaagatc tttgacagct agctcagtcc taggtataat actagtggaa gagccaaagt
     1501 caacacttat agacaaatat gatctttcta accttcacga aatagcatca gggggagcac
     1561 ctttgtctaa ggaagtagga gaggcagttg caaagaggtt tcatttaccc ggaattaggc
     1621 aaggatatgg gttaacagaa accacatctg caattttaat tacacctgaa ggagatgata
     1681 aaccaggagc tgttgggaag gttgttcctt tttttgaggc taaagttgtt gatttagata
     1741 ctggaaagac tttaggagtt aatcaaagag gcgagttatg tgtaagagga cctatgataa
     1801 tgtcaggata cgtaaataat ccagaagcta caaatgcatt gatagataaa gatggttggt
     1861 tacactcagg cgatatagct tattgggacg aagatgaaca cttttttata gttgatagat
     1921 tgaaatcttt gattaagtat aaaggttatc aagtagctcc agcagaattg gaaagtattt
     1981 tattgcaaca cccagctctt ccgttttaga gctagaaata gcaagttaaa ataaggctag
     2041 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttggcgc gccaaataat
     2101 gcccgtgtcc ctgttgaata gggacacggt tttttaacaa tttttttatt cctaggcctt
     2161 agtggccggt acccggggat ccttattact ggccgctttc tagcctggat acttgtctgg
     2221 ctagttgcat tagagagtca gctagagccc tagggctagc tgtttgtgta ttcggagcca
     2281 gtgtagtttt tctttttact aacgatacgg gtaataattg attgcctttt acagcctgac
     2341 cttgagacaa ttgcaaaagt ctgtccacag aggtttgacc taataatcta aaatcttgtt
     2401 tgatagtagt aagtggaggt atgtagcaac tagaatcttc tgtatcatca taacccacaa
     2461 ctgaaatatc agccccaact ctaagtccac tttcagtaat agctcgcata gctcctaaag
     2521 ccatttgatc attagcaact aacatagcag tagggacaat gccttcattt aacatttgca
     2581 ttgtttgctg aaatcctgac atagcagacc aatctccttc tctttctgct attggttgta
     2641 tttgatttct tgttaaatac ttgtgccatc ctgctaatct caatctggca cttactgaac
     2701 ttaagggacc tgcaagtagt gcaatttgtt gatggcctaa tgcaacaagg tgttcaactc
     2761 ctaaccttgt tccatcttcg tgagaaaaaa ttatagagtt tattggggtt tggtccgaca
     2821 catccaaaaa caatgctgga acattagtac atgctgcttc aactgcaatc gcatcttggt
     2881 catcaagagg ataattaatt ataagtccac taacacgttg agctaataaa ttatgtactg
     2941 ctgccttaca agcctctaca cctgatctct caaccattga tacaacaacg cttgcaccaa
     3001 gttgatcagc tctcgattta attgcggcta caatttgact aggcgcatgt aatgcaagtg
     3061 atgatgtggc aactccaatt aaaagtgatt gtttccccgc aagttgctga gcaacgcgat
     3121 ttggtatata attcaactct gccatagctg cttcaacctt ctctcttgtt tttgcagata
     3181 catgacttgc ttggttcaca actctcgaaa ccgtttggta gctaaccccg gcatattcag
     3241 caacatcata caaagtaaca ggtttcatgg atcattcctc catgataaaa tttaaatttc
     3301 tgactttggc aaatcctttt gcctgttaaa aagaatagca caaaaattaa aaaaaaagaa
     3361 acgcaaatag caatagcatt aaaaataatt attattattt atttctttga ttaaaatcaa
     3421 aagcctttaa tgtattttct tgatctttca tgcgcttaac ggtaaaatcc aaggtattaa
     3481 gttcaccttc cacttttctc tctctatctt ctctagaaaa tcataaaaaa tttatttgct
     3541 ttgtgagcgg ataacaatta taacagattc aattgtgagc ggataacaat ttcacacaga
     3601 attcattaaa gaggagaaat tacatatgga taagaaatac tcaataggct tagctatcgg
     3661 cacaaatagc gtcggatggg cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt
     3721 caaggttctg ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt
     3781 atttgacagt ggagagacag cggaagcgac tcgtctcaaa cggacagctc gtagaaggta
     3841 tacacgtcgg aagaatcgta tttgttatct acaggagatt ttttcaaatg agatggcgaa
     3901 agtagatgat agtttctttc atcgacttga agagtctttt ttggtggaag aagacaagaa
     3961 gcatgaacgt catcctattt ttggaaatat agtagatgaa gttgcttatc atgagaaata
     4021 tccaactatc tatcatctgc gaaaaaaatt ggtagattct actgataaag cggatttgcg
     4081 cttaatctat ttggccttag cgcatatgat taagtttcgt ggtcattttt tgattgaggg
     4141 agatttaaat cctgataata gtgatgtgga caaactattt atccagttgg tacaaaccta
     4201 caatcaatta tttgaagaaa accctattaa cgcaagtgga gtagatgcta aagcgattct
     4261 ttctgcacga ttgagtaaat caagacgatt agaaaatctc attgctcagc tccccggtga
     4321 gaagaaaaat ggcttatttg ggaatctcat tgctttgtca ttgggtttga cccctaattt
     4381 taaatcaaat tttgatttgg cagaagatgc taaattacag ctttcaaaag atacttacga
     4441 tgatgattta gataatttat tggcgcaaat tggagatcaa tatgctgatt tgtttttggc
     4501 agctaagaat ttatcagatg ctattttact ttcagatatc ctaagagtaa atactgaaat
     4561 aactaaggct cccctatcag cttcaatgat taaacgctac gatgaacatc atcaagactt
     4621 gactctttta aaagctttag ttcgacaaca acttccagaa aagtataaag aaatcttttt
     4681 tgatcaatca aaaaacggat atgcaggtta tattgatggg ggagctagcc aagaagaatt
     4741 ttataaattt atcaaaccaa ttttagaaaa aatggatggt actgaggaat tattggtgaa
     4801 actaaatcgt gaagatttgc tgcgcaagca acggaccttt gacaacggct ctattcccca
     4861 tcaaattcac ttgggtgagc tgcatgctat tttgagaaga caagaagact tttatccatt
     4921 tttaaaagac aatcgtgaga agattgaaaa aatcttgact tttcgaattc cttattatgt
     4981 tggtccattg gcgcgtggca atagtcgttt tgcatggatg actcggaagt ctgaagaaac
     5041 aattacccca tggaattttg aagaagttgt cgataaaggt gcttcagctc aatcatttat
     5101 tgaacgcatg acaaactttg ataaaaatct tccaaatgaa aaagtactac caaaacatag
     5161 tttgctttat gagtatttta cggtttataa cgaattgaca aaggtcaaat atgttactga
     5221 aggaatgcga aaaccagcat ttctttcagg tgaacagaag aaagccattg ttgatttact
     5281 cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat
     5341 agaatgtttt gatagtgttg aaatttcagg agttgaagat agatttaatg cttcattagg
     5401 tacctaccat gatttgctaa aaattattaa agataaagat tttttggata atgaagaaaa
     5461 tgaagatatc ttagaggata ttgttttaac attgacctta tttgaagata gggagatgat
     5521 tgaggaaaga cttaaaacat atgctcacct ctttgatgat aaggtgatga aacagcttaa
     5581 acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa ttgattaatg gtattaggga
     5641 taagcaatct ggcaaaacaa tattagattt tttgaaatca gatggttttg ccaatcgcaa
     5701 ttttatgcag ctgatccatg atgatagttt gacatttaaa gaagacattc aaaaagcaca
     5761 agtgtctgga caaggcgata gtttacatga acatattgca aatttagctg gtagccctgc
     5821 tattaaaaaa ggtattttac agactgtaaa agttgttgat gaattggtca aagtaatggg
     5881 gcggcataag ccagaaaata tcgttattga aatggcacgt gaaaatcaga caactcaaaa
     5941 gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca aagaattagg
     6001 aagtcagatt cttaaagagc atcctgttga aaatactcaa ttgcaaaatg aaaagctcta
     6061 tctctattat ctccaaaatg gaagagacat gtatgtggac caagaattag atattaatcg
     6121 tttaagtgat tatgatgtcg atgccattgt tccacaaagt ttccttaaag acgattcaat
     6181 agacaataag gtcttaacgc gttctgataa aaatcgtggt aaatcggata acgttccaag
     6241 tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg ccaagttaat
     6301 cactcaacgt aagtttgata atttaacgaa agctgaacgt ggaggtttga gtgaacttga
     6361 taaagctggt tttatcaaac gccaattggt tgaaactcgc caaatcacta agcatgtggc
     6421 acaaattttg gatagtcgca tgaatactaa atacgatgaa aatgataaac ttattcgaga
     6481 ggttaaagtg attaccttaa aatctaaatt agtttctgac ttccgaaaag atttccaatt
     6541 ctataaagta cgtgagatta acaattacca tcatgcccat gatgcgtatc taaatgccgt
     6601 cgttggaact gctttgatta agaaatatcc aaaacttgaa tcggagtttg tctatggtga
     6661 ttataaagtt tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc
     6721 aaccgcaaaa tatttctttt actctaatat catgaacttc ttcaaaacag aaattacact
     6781 tgcaaatgga gagattcgca aacgccctct aatcgaaact aatggggaaa ctggagaaat
     6841 tgtctgggat aaagggcgag attttgccac agtgcgcaaa gtattgtcca tgccccaagt
     6901 caatattgtc aagaaaacag aagtccagac aggcggattc tccaaggagt caattttacc
     6961 aaaaagaaat tcggacaagc ttattgctcg taaaaaagac tgggatccaa aaaaatatgg
     7021 tggttttgat agtccaacgg tagcttattc agtcctagtg gttgctaagg tggaaaaagg
     7081 gaaatcgaag aagttaaaat ccgttaaaga gttactaggg atcacaattc tggaaagaag
     7141 ttcctttgaa aaaaatccga ttgacttttt agaagctaaa ggatataagg aagttaaaaa
     7201 agacttaatc attaaactac ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg
     7261 gatgctggct agtgccggag aattacaaaa aggaaatgag ctggctctgc caagcaaata
     7321 tgtgaatttt ttatatttag ctagtcatta tgaaaagttg aagggtagtc cagaagataa
     7381 cgaacaaaaa caattgtttg tggagcagca taagcattat ttagatgaga ttattgagca
     7441 aatcagtgaa ttttctaagc gtgttatttt agcagatgcc aatttagata aagttcttag
     7501 tgcatataac aaacatagag acaaaccaat acgtgaacaa gcagaaaata ttattcattt
     7561 atttacgttg acgaatcttg gagctcccgc tgcttttaaa tattttgata caacaattga
     7621 tcgtaaacga tatacgtcta caaaagaagt tttagatgcc actcttatcc atcaatccat
     7681 cactggtctt tatgaaacac gcattgattt gagtcagcta ggaggtgact aataaaagct
     7741 tgatcagatc tcagcttttt tttgaagtgc ctggcagtaa gttgaaaaaa tttgaaataa
     7801 acttggacta tgttaataaa aaaatacaaa ttaacgaaca aaaagatttg aattattata
     7861 taatttagtt aaaagcaatt ttaaatgagg gaggtttcca tatgagggaa gcggtgatcg
     7921 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     7981 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     8041 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     8101 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     8161 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     8221 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     8281 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     8341 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     8401 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     8461 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     8521 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     8581 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     8641 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgacgtc
     8701 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct catgaccaaa atcccttaac
     8761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8941 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcactag
     9481 atccccggca agttcatcct tagggttttc atatctctta aaaagtcaat ttctgcttga
     9541 ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct tgagtattga
     9601 attttttctt atatataaat atatagcaaa aactattttt gccaactttt ttacaaaaaa
     9661 ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact tagtaaagcc
     9721 ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata ttaatagtat
     9781 agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc atacaaaata
     9841 caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac ctaatctcta
     9901 aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt aaaagttttt
     9961 taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta aatttggatt
    10021 tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat ggttttattg
    10081 gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa ttataagaat
    10141 ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc agtgcagttg
    10201 ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt ataaaatcaa
    10261 gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct tttaaaattt
    10321 taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac ttttccttta
    10381 gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca acataagtat
    10441 ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt ttcctttatt
    10501 tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt aatatccact
    10561 taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca tgtttttcta
    10621 aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt tcttttctta
    10681 ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac ctaaattcta
    10741 tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca tactttacaa
    10801 aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg tccccgtctt
    10861 tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt tcaaaaaaaa
    10921 ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat atttttgtgt
    10981 gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct tttggaaatt
    11041 taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt aatccttata
    11101 tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc attagttcta
    11161 ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt tggttattca
    11221 aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt agttgttcaa
    11281 taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa ttttcttttt
    11341 t
//
