LOCUS       sequence-31518-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PDXK
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attccctctg aaatattttg tttaacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtt ccgcgtggta gtcgggtgct ctccatacag
      121 agccacgtca tccgcggcta cgtgggcaac cgggcggcca cgttcccgct gcaggttttg
      181 ggatttgaga ttgacgcggt gaactctgtc cagttttcaa accacacagg ctatgcccac
      241 tggaagggcc aagtgctgaa ttcagatgag ctccaggagt tgtacgaagg cctgaggctg
      301 aacaacatga ataaatatga ctacgtgctc acaggttata cgagggacaa gtcgttcctg
      361 gccatggtgg tggacattgt gcaggagctg aagcagcaga accccaggct ggtgtacgtg
      421 tgtgatccag tcttgggtga caagtgggac ggcgaaggct cgatgtacgt cccggaggac
      481 ctccttcccg tctacaaaga aaaagtggtg ccgcttgcag acattatcac gcccaaccag
      541 tttgaggccg agttactgag tggccggaag atccacagcc aggaggaagc cttgcgggtg
      601 atggacatgc tgcactctat gggccccgac accgtggtca tcaccagctc cgacctgccc
      661 tccccgcagg gcagcaacta cctgattgtg ctggggagtc agaggaggag gaatcccgct
      721 ggctccgtgg tgatggaacg catccggatg gacattcgca aagtggacgc cgtctttgtg
      781 ggcactgggg acctgtttgc tgccatgctc ctggcgtgga cacacaagca ccccaataac
      841 ctcaaggtgg cctgtgagaa gaccgtgtct accttgcacc acgttctgc
//
