LOCUS       sequence-315181-       12381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express human YTHDF1(264-559) with D401N mutation and
            CRY2olig-BFP at the N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12381)
  AUTHORS   Fu Y, Zhuang X
  TITLE     m(6)A-binding YTHDF proteins promote stress granule formation.
  JOURNAL   Nat Chem Biol. 2020 May 25. pii: 10.1038/s41589-020-0524-y. doi: 
            10.1038/s41589-020-0524-y.
  PUBMED    32451507
REFERENCE   2  (bases 1 to 12381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 25. pii: 10.1038/s41589-020-0524-y. doi: 
            10.1038/s41589-020-0524-y."
COMMENT     Sequence Label: CRY2olig-BFP-YTHDF1(D401N)-C
FEATURES             Location/Qualifiers
     source          1..12381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1226..2719
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     CDS             2792..3487
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    4452..5040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4505..4525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4923..4934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5043..5059)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5044..5060)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5565..5745
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5771..5788)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5777..6001
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6047..6475
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6134..6153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6344..6365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6484..6504)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6489..6818
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6669..6804
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6731..6750
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6866..6913
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6932..7306
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7436..7557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7473..7492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7527..7546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7606..7622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7606..7622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7619..7641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7630..7646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7654..7660,7661..7678,7679..7684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7699..7720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7837..7854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8008..8596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8088..8107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8767..9558,9559..9627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9390..9409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9628..9732)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9807..9826)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9998..10377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10379..10577
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10532..10552
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10595..10775
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10822..10947
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11440..11673
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11858..11902
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12200..12317
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acaggatccg ccaccatgaa gatggacaaa aagactatag tttggtttag
     1261 aagagaccta aggattgagg ataatcctgc attagcagca gctgctcacg aaggatctgt
     1321 ttttcctgtc ttcatttggt gtcctgaaga agaaggacag ttttatcctg gaagagcttc
     1381 aagatggtgg atgaaacaat cacttgctca cttatctcaa tccttgaagg ctcttggatc
     1441 tgacctcact ttaatcaaaa cccacaacac gatttcagcg atcttggatt gtatccgcgt
     1501 taccggtgct acaaaagtcg tctttaacca cctctatgat cctgtttcgt tagttcggga
     1561 ccataccgta aaggagaagc tggtggaacg tgggatctct gtgcaaagct acaatggaga
     1621 tctattgtat gaaccgtggg agatatactg cgaaaagggc aaacctttta cgagtttcaa
     1681 ttcttactgg aagaaatgct tagatatgtc gattgaatcc gttatgcttc ctcctccttg
     1741 gcggttgatg ccaataactg cagcggctga agcgatttgg gcgtgttcga ttgaagaact
     1801 agggctggag aatgaggccg agaaaccgag caatgcgttg ttaactagag cttggtctcc
     1861 aggatggagc aatgctgata agttactaaa tgagttcatc gagaagcagt tgatagatta
     1921 tgcaaagaac agcaagaaag ttgttgggaa ttctacttca ctactttctc cgtatctcca
     1981 tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc cggatgaaac aaattatatg
     2041 ggcaagagat aagaacagtg aaggagaaga aagtgcagat ctttttctta ggggaatcgg
     2101 tttaagagag tattctcggt atatatgttt caacttcccg tttactcacg agcaatcgtt
     2161 gttgagtcat cttcggtttt tcccttggga tgctgatgtt gataagttca aggcctggag
     2221 acaaggcagg accggttatc cgttggtgga tgccggaatg agagagcttt gggctaccgg
     2281 atggatgcat aacagaataa gagtgattgt ttcaagcttt gctgtgaagt ttcttctcct
     2341 tccatggaaa tggggaatga agtatttctg ggatacactt ttggatgctg atttggaatg
     2401 tgacatcctt ggctggcagt atatctctgg gagtatcccc gatggccacg agcttgatcg
     2461 cttggacaat cccgcgttac aaggcgccaa atatgaccca gaaggtgagt acataaggca
     2521 atggcttccc gagcttgcga gattgccaac tgaatggatc catcatccat gggacgctcc
     2581 tttaaccgta ctcaaagctt ctggtgtgga actcggaaca aactatgcga aacccattgt
     2641 agacatcgac acagctcgtg agctactagc taaagctatt tcaagaaccc gtggagcaca
     2701 gatcatgatc ggagcagcag cccgggatcc accggtcgcc accgatccag gtggtggttc
     2761 aggaggcggc ggaggtggag gtggaagcgg tagcgagctg attaaggaga acatgcacat
     2821 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     2881 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     2941 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     3001 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     3061 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     3121 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     3181 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     3241 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     3301 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     3361 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     3421 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     3481 gcttaatggt ggaggtagtg gaagtggcgg aagcggaagc ctaggcgcgc ccataaagca
     3541 taacatggac attggcacct gggataacaa ggggcctgtg ccgaaggccc cagtccccca
     3601 gcaggcaccc tctccacagg ctgccccaca gccccagcag gtggctcagc ctctcccagc
     3661 acagccccca gctttggctc aaccgcagta tcagagccct cagcagccac cccagacccg
     3721 ctgggttgcc ccacgcaaca gaaacgcggc gtttgggcag agcggagggg ctggcagcga
     3781 tagcaactct cctggaaacg tccagcctaa ttctgccccc agcgtcgaat cccaccccgt
     3841 ccttgaaaaa ctgaaggctg ctcacagcta caacccgaaa gagtttgagt ggaatctgaa
     3901 aagcgggcgt gtgttcatca tcaagagcta ctctgaggac aacatccacc gctccattaa
     3961 gtactccatc tggtgtagca cagagcacgg caacaagcgc ctggacagcg ccttccgctg
     4021 catgagcagc aaggggcccg tctacctgct cttcagcgtc aatgggagtg ggcatttttg
     4081 tggggtggcc gagatgaagt cccccgtgga ctacggcacc agtgccgggg tctggtctca
     4141 ggacaagtgg aaggggaagt ttgatgtcca gtggattttt gttaaggatg tacccaataa
     4201 ccagctccgg cacatcaggc tggagaataa cgacaacaaa ccggtcacaa actcccggga
     4261 cacccaggag gtgcccttag aaaaagccaa gcaagtgctg aaaattatca gttcctacaa
     4321 gcacacaacc tccatcttcg acgactttgc tcactacgag aagcgccagg aggaggagga
     4381 ggtggtgcgc aaggaacggc agagtcgaaa caaacaacct gcaggttgaa ttcgatatca
     4441 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4501 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4561 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4621 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4681 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4741 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4801 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4861 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4921 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4981 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5041 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     5101 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     5161 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5221 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     5281 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     5341 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     5401 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     5461 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     5521 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     5581 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5641 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5701 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     5761 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5821 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5881 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5941 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6001 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6061 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6121 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6181 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6241 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6301 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6361 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6421 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6481 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6541 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6661 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6721 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6781 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6841 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     6901 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     6961 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     7021 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     7081 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     7141 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     7201 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     7261 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     7321 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7381 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7441 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7501 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7561 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7621 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9361 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9541 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9601 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9661 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9721 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     9781 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9841 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     9901 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     9961 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
    10021 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    10081 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    10141 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    10201 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    10261 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    10321 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
    10381 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    10441 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
    10501 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
    10561 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
    10621 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10681 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10741 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    10801 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    10861 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    10921 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    10981 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    11041 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    11101 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    11161 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    11221 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    11281 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    11341 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    11401 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    11461 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    11521 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    11581 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    11641 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    11701 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    11761 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    11821 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    11881 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    11941 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    12001 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    12061 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    12121 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
    12181 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    12241 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    12301 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta
    12361 acccgtgtcg gctccagatc t
//
