LOCUS       sequence-31519-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NME3
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             627..644
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(697..715)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      711..758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt atgatctgcc tggtgctgac catcttcgct
      121 aacctcttcc ccgcggcctg caccggcgca cacgaacgca ccttcctggc cgtgaagccg
      181 gacggcgtgc agcggcggct ggtgggcgag attgtgcggc gcttcgagag gaagggcttc
      241 aagttggtgg cgctgaagct ggtgcaggcc tccgaggagc tgctgcgtga gcactacgcc
      301 gagctgcgtg aacgcccgtt ctacggccgc cttgtcaagt atatggcctc cgggccggtg
      361 gtggccatgg tttggcaggg gctggacgtg gtgcgcacct cgcgggcgct catcggagcc
      421 acgaacccgg ccgacgcccc gcccggcacc atccgcgggg atttctgcat cgaggttggc
      481 aagaacctga ttcacggcag cgactcggtg gagagtgccc gccgcgagat cgctctctgg
      541 ttccgcgcag acgagctcct ctgctgggag gacagcgctg ggcactggct gtatgagtga
      601 tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      661 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      721 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      781 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      841 gcgcagcgtg accgctacac ttgccagcg
//
