LOCUS       sequence-31520-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KIFC3
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag taaagggaac atccgagtga ttgctcgtgt
      121 ccggccagtc accaaagagg atggggaagg acctgaggcc accaatgctg tgactttcga
      181 tgccgacgac gactccatca tccacctgct gcacaagggc aagcctgtgt ccttcgagct
      241 ggacaaggtc ttctccccac aggcctcgca gcaggacgtg ttccaggagg tgcaggccct
      301 ggtcacctct tgcattgatg gcttcaatgt ctgcatcttt gcgtacggcc agacgggcgc
      361 cggcaagacg tacacgatgg aggggaccgc tgagaaccca ggtatcgacc agcgggccct
      421 gcagctgctc ttctccgagg tgcaggagaa ggcgtctgac tgggagtaca ccatcaccgt
      481 cagcgctgcg gagatctaca atgaggtcct cagggacctg ctagggaaag agcctcagga
      541 aaaactggag atccggctgt gcccagatgg cagtgggcag ctgtatgtac cagggctgac
      601 tgagttccaa gtgcagagcg tggacgacat caacaaggtg tttgagtttg gccacactaa
      661 tcgcacgacc gagttcacca acctgaacga gcacagctcc cgctcgcacg cgctgctcat
      721 cgtgacggtg cgaggcgtgg actgcagcac aggcctccgc accacgggga agctgaacct
      781 ggtggacttg gctggctcgg agcgcgtggg caagtcgggg gccgagggca gccgcctgcg
      841 ggaggcgcag cacatcaaca agtcgctgtc ggctctgggg gacgtcattg ctgccctgcg
      901 ct
//
