LOCUS       sequence-315215-        9812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. 
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Dangerfield TL, Huang NZ, Johnson KA
  TITLE     Remdesivir Is Effective in Combating COVID-19 because It Is a Better
            Substrate than ATP for the Viral RNA-Dependent RNA Polymerase.
  JOURNAL   iScience. 2020 Nov 28:101849. doi: 10.1016/j.isci.2020.101849.
  PUBMED    33283177
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience. 
            2020 Nov 28:101849. doi: 10.1016/j.isci.2020.101849."
COMMENT     Sequence Label: pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
FEATURES             Location/Qualifiers
     source          1..9812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(214..927)
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     RBS             1016..1024
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      1936..2022
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2114..2141
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2235..2251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2235..2251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2259..2275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2283..2289,2290..2307,2308..2313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2328..2349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3233..3319
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3412..4494)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4456..4475
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4495..4572)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(4656..4678)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4764..4904
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4819..4838
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5090..5678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5170..5189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5785..6600)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5785..6600)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6508..6527
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6592..6611
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     6672..6690
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(6714..6728,6729..6734,6735..6751,6752..6758)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    6734..6750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6766..6782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6801..6812
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      9640..9734
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gaaccacgga acacaatcga acttttgctg agttgactcc ccgcgcggac attaattgcg
       61 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      121 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc atagacgtct ttgaattgtt
      181 atcagctatg cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg
      241 actagcgata actttcccca caacggaaca actctcattg catgggatca ttgggtactg
      301 tgggtttagt ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa
      361 ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc
      421 aacgagaatt aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga
      481 attaccttca acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca
      541 tctctccgca tcacctttgg taaaggttct aagctcaggt gagaacatcc ctgcctgaac
      601 atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc
      661 atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag
      721 aatttttgta agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc
      781 accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag
      841 ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg
      901 tgttaatggt ttcttttttg tgctcatacg ttaaatctat caccgcaagg gataaatatc
      961 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgca tgtactaagg
     1021 aggttcatat gtctaagatg agtgatgtaa aatgtacatc agtggttctg ctgtcggtac
     1081 tccaacagct gcgtgttgag agctctagca agctgtgggc ccaatgtgtt cagctgcaca
     1141 acgacatcct gttggcgaaa gataccacgg aagcgttcga gaaaatggtc agcctgttgt
     1201 ccgtgttgtt aagcatgcag ggtgctgtgg atattaataa gctttgcgag gaaatgctgg
     1261 acaaccgcgc aaccctgcaa tgagtttaaa caaggaaaat aaaatatggc tatagcatct
     1321 gaattttcaa gtctacccag ttatgcggcg tttgctaccg cccaagaggc gtacgagcag
     1381 gcggttgcta atggtgattc tgaagtcgtg ctgaagaaac tgaaaaagtc cctgaacgtg
     1441 gccaaatccg agttcgatcg tgacgccgct atgcaacgta aattggaaaa aatggcggac
     1501 caggctatga cccagatgta caagcaggca cgttctgaag ataagcgcgc gaaagttacc
     1561 agcgcaatgc aaaccatgct gttcacgatg ctgcgtaagc tggacaacga tgcgttgaac
     1621 aacatcatca ataatgcaag agacggttgt gttccgttga acattattcc gttgaccacc
     1681 gcggcgaaac ttatggtcgt gatcccggat tataatacct acaagaacac gtgcgacggc
     1741 accacgttta cctatgcgag cgcgttatgg gaaattcaac aagttgttga cgcagacagc
     1801 aagatcgtgc agctgagcga gatcagcatg gataacagcc cgaatctggc atggccactg
     1861 attgtgactg ctctccgcgc gaactcggcc gtaaaactgc agtaagaatt catgcagtag
     1921 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1981 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     2041 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     2101 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     2161 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat agatctaagc
     2221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2281 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2521 cactcaaagg cggtaatacg gttatccaca gaatcagatg gtcttgccgt gtcgaaggca
     2581 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2641 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2701 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
     2761 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2821 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2881 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2941 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     3001 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     3061 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     3121 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     3181 agttattggt gcccttaaac gcctggggta atgactctct agcttgaggc atcaaataaa
     3241 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3301 tctcctgagt aggacaaatc cgccctctag attacgtgca gtcgatgata agctgtcaaa
     3361 catgagaatt gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     3421 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3481 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     3541 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     3601 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     3661 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     3721 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     3781 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     3841 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     3901 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     3961 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     4021 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     4081 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     4141 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     4201 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     4261 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     4321 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     4381 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     4441 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     4501 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca
     4561 ttcgatggtg tcggaatttc gggcagcgtt gggtcctggc cacgggtgcg catgatctag
     4621 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4681 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4741 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4801 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4861 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     4921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4981 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5521 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5641 gcaagcagca gattacgcgc agaaaaaagg gatctcaaga agatcctttg atcttttcta
     5701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atggcgttaa
     5761 gggattttgg tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa
     5821 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     5881 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     5941 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     6001 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     6061 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     6121 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     6181 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     6241 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     6301 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     6361 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     6421 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     6481 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     6541 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat
     6601 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg accatgacat
     6661 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa
     6721 aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac
     6781 aatttcacac agaattcatt aaagaggaga aaactagtat gtcagctgat gcacaaagtt
     6841 ttctaaatag ggtctgtggc gtttctgccg cgcgtttaac tccgtgtgga accggcacca
     6901 gcaccgacgt tgtttaccgc gcgtttgata tttataatga taaggtggct ggctttgcga
     6961 aattcctgaa gacgaactgt tgtcgttttc aagaaaaaga tgaggatgat aacctgattg
     7021 attcctactt cgtcgtgaaa cgtcatacct ttagcaatta tcagcacgag gagaccatct
     7081 acaatttgct taaggactgt ccggcagttg cgaagcacga tttcttcaag ttccgcatcg
     7141 acggtgacat ggtgccgcat atttcccgtc agcgtttgac gaagtatacc atggcagatt
     7201 tggtctacgc gttgcgtcat tttgacgagg gcaactgcga tacgctgaaa gagatcctgg
     7261 ttacctataa ctgctgcgac gacgattatt tcaacaagaa agactggtat gacttcgtgg
     7321 agaacccgga tatcctgcgt gtgtatgcca acctgggcga acgtgttcgt caggcactgc
     7381 tgaagactgt acagttttgt gatgccatgc gtaatgctgg tattgttggt gtcctgaccc
     7441 tggacaacca ggaccttaat ggtaactggt atgatttcgg cgacttcatt caaaccaccc
     7501 cgggttctgg tgttccggtt gtcgacagct actatagcct gttgatgccc atcttaacgc
     7561 tgacccgtgc gctgacggcg gaaagccacg tggacaccga cctcaccaag ccgtatatca
     7621 aatgggattt gttgaaatac gacttcaccg aagagcgctt gaagctgttc gaccgttact
     7681 tcaagtactg ggaccaaacg tatcatccaa actgcgttaa ctgcctggac gaccgttgta
     7741 tcctgcactg cgcgaacttt aatgttctgt tttccactgt gttcccgccg accagctttg
     7801 gtccgctggt acgcaaaatc ttcgtggatg gcgtcccgtt cgtggtgagc accggttatc
     7861 acttccgcga actgggtgtg gttcataacc aagacgtgaa cctccacagc tcacgtttgt
     7921 cctttaaaga gctgctcgtt tacgcggctg accctgcaat gcatgcagcg agcggtaacc
     7981 tcctgctcga taaacggacc acctgcttca gcgttgctgc cttaacgaac aacgtggcgt
     8041 ttcaaaccgt taaaccgggc aacttcaaca aagatttcta cgacttcgcg gtttcaaaag
     8101 ggttctttaa ggagggttcg tccgtagagc tgaagcactt ttttttcgcg caggatggca
     8161 acgcggcaat ctccgattac gactactaca gatacaacct gccaacgatg tgcgacatcc
     8221 gtcaactgtt gtttgttgtc gaggttgttg acaaatactt cgattgttac gacggcggtt
     8281 gcattaatgc aaatcaagtt attgtcaaca acttggacaa gagtgccgga tttccgttta
     8341 ataagtgggg taaagcacgc ctgtactatg atagcatgag ctatgaggac caggacgctc
     8401 tgttcgcgta caccaaacgt aatgtgatcc cgacaattac ccagatgaat ttgaagtacg
     8461 cgatcagcgc gaaaaatcga gcccgcaccg tcgctggtgt ttcgatctgc agcacgatga
     8521 ccaacagaca attccaccag aagctgttaa agtctatcgc agctaccaga ggcgcgaccg
     8581 ttgtgattgg tacaagcaaa ttttatggcg gttggcataa tatgctgaag accgtttata
     8641 gcgatgtgga gaatccgcac ctgatgggtt gggactaccc gaaatgcgac cgcgctatgc
     8701 cgaatatgtt gcgtattatg gctagcctgg tgctggcacg taaacacacc acatgctgca
     8761 gcctgtcaca tcgtttttat cgtctagcca acgagtgcgc tcaggtcctg tctgaaatgg
     8821 ttatgtgcgg tggctcccta tacgttaagc caggtggcac cagctctggc gatgcgacca
     8881 ctgcgtatgc gaacagcgtt ttcaacattt gtcaggccgt gaccgcgaac gtgaacgccc
     8941 tgttgtctac cgacggtaac aagatcgcag ataaatacgt gcgcaacctg cagcaccgtc
     9001 tgtatgaatg cttgtaccgc aaccgcgatg ttgataccga tttcgtcaat gagttttacg
     9061 cgtatctgcg taagcacttc agcatgatga tcctgtccga tgacgcggtg gtgtgcttta
     9121 acagcacgta cgcgagtcag ggcctggttg cgtccattaa aaattttaag tcggtgctgt
     9181 actaccagaa taacgtgttc atgtccgaag ctaagtgctg gactgaaact gacctgacga
     9241 aaggtccgca tgagttctgc tcgcaacaca ccatgctcgt gaaacagggt gatgactacg
     9301 tatacttgcc ttatccagat ccgagccgta ttctgggtgc cggctgcttt gtggacgaca
     9361 tcgtgaagac cgacggcact ctgatgattg aacgcttcgt gagcttagcg atcgatgcgt
     9421 atccgctgac caaacacccg aaccaagagt atgccgatgt gtttcatctg tatttgcaat
     9481 atattcgtaa actgcatgat gaactgaccg gtcacatgct ggatatgtat agcgttatgc
     9541 tgaccaacga taatacgtcc cgttattggg aaccggaatt ttacgaggca atgtataccc
     9601 cgcacaccgt gctgcaatga ggatccttag ctgagcttgg actcctgttg atagatccag
     9661 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     9721 ttattggtga gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg
     9781 gagaaaaaaa tcactggata taccaccgtt gt
//
