LOCUS       sequence-31523-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARL10C
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             632..649
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(702..720)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      716..763
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(887..906)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag taaggaagag atggagctga
      121 cgctcgtggg gctgcagtac tcgggcaaga ccaccttcgt caatgtcatc gcgtcaggtc
      181 aattcagtga agatatgata cccacagtgg gcttcaacat gaggaaggta actaaaggta
      241 acgtcacaat aaagatctgg gacataggag gacaaccccg atttcgaagc atgtgggagc
      301 ggtattgcag aggagtcaat gctattgttt acatgataga tgctgcagat cgtgaaaaga
      361 tagaagcttc ccgaaatgag ctacataatc ttctagataa accacagtta caaggaattc
      421 cagtgctagt gcttggaaac aagagagatc ttcctaatgc cttggatgag aaacagctaa
      481 ttgaaaaaat gaatctgtct gctattcagg atagagaaat ttgctgctat tcaatttctt
      541 gcaaagaaaa ggataatata gatatcacac ttcagtggct tattcagcat tcaaaatcta
      601 gatgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      661 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      721 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      781 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      901 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tc
//
