LOCUS       sequence-31524-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AK3L1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             816..833
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agaaattccc tctgaatatt ttgtttaact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gttccgcgtg gtagtatggg ggcgtccgcg
      121 cggctgctgc gagcggtgat catgggggcc ccgggctcgg gcaagggcac cgtgtcgtcg
      181 cgcatcacta cacacttcga gctgaagcac ctctccagcg gggacctgct ccgggacaac
      241 atgctgcggg gcacagaaat tggcgtgtta gccaaggctt tcattgacca agggaaactc
      301 atcccagatg atgtcatgac tcggctggcc cttcatgagc tgaaaaatct cacccagtat
      361 agctggctgt tggatggttt tccaaggaca cttccacagg cagaagccct agatagagct
      421 tatcagatcg acacagtgat taacctgaat gtgccctttg aggtcattaa acaacgcctt
      481 actgctcgct ggattcatcc cgccagtggc cgagtctata acattgaatt caaccctccc
      541 aaaactgtgg gcattgatga cctgactggg gagcctctca ttcagcgtga ggatgataaa
      601 ccagagacgg ttatcaagag actaaaggct tatgaagacc aaacaaagcc agtcctggaa
      661 tattaccaga aaaaaggggt gctggaaaca ttctccggaa cagaaaccaa caagatttgg
      721 ccctatgtat atgctttcct acaaactaaa gttccacaaa gaagccagaa agcttcagtt
      781 actccatgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      841 cggctgctaa caaagcccga aagg
//
