LOCUS       sequence-315249-        3636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Brent AE, Braun T, Tabin CJ
  TITLE     Genetic analysis of interactions between the somitic muscle, 
            cartilage and tendon cell lineages during mouse development.
  JOURNAL   Development. 2005 Feb . 132(3):515-28.
  PUBMED    15634692
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2005 Feb . 132(3):515-28."
COMMENT     Sequence Label: pBS cCollagen XII (CT#1051)
FEATURES             Location/Qualifiers
     source          1..3636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1405..1421)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1406..1422)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1451..1471)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1451..1469)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1490..1506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1490..1506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1503..1525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1514..1530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1538..1544,1545..1562,1563..1568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1583..1604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1721..1738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1892..2480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1972..1991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2651..3442,3443..3511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3274..3293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3512..3616)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct
      661 ccaccgcggt ggcggccgcg gctgcagtcc ggcccctggg cgagtgaagg ggtacaaagt
      721 tactttccac cctactgaag atgacaggaa tcttggagaa ttaattgtgg gaccctatga
      781 cagcactgtg gttttggagg aacttagggc aggaactacc tataaggtaa atgtttttgg
      841 aatgtttgat ggaggagaaa gcaaccccct ggttggacaa gagatgacca ctctttcaga
      901 cactaccacg gagccatttt tatctagagg tttagaatgt agaaccagag cagaagctga
      961 tatcgtgcta ctggtggatg gctcatggag tatcgggagg ccaaatttta aaactgtcag
     1021 gaacttcatt tcccgtattg ttgaagtttt tgatattggt cctgacaaag tacagattgg
     1081 tcttgcacag tatagcggag atcccaggac agaatggaat ctcaatgctt accgaacgaa
     1141 agaggctttg ttagaggcag taaccaactt accatacaaa ggaggcaata ctctaacagg
     1201 aatggccttg gatttcatct taaaaaacaa ctttaaacaa gaagcaggct tgaggcccag
     1261 agctcgcaaa attggtgttc tcattactga tggtaaatca caagacgacg tagtgacccc
     1321 ttcaagaaga ctcagagatg agggagtaga actttatgca attggtatta aaaacgcaaa
     1381 aaaagaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca
     1441 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     1501 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc gggagcataa
     1561 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1621 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1681 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1741 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1801 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1861 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1921 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1981 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2041 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2101 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2161 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2221 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2281 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2341 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2401 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2461 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2521 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2581 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2641 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2701 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2761 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2821 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2881 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2941 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3001 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3061 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3121 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3181 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3241 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3301 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3361 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3421 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3481 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3541 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3601 aaataggggt tccgcgcaca tttccccgaa aagtgc
//
