LOCUS       sequence-31525-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB30
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             676..693
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(746..764)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      760..807
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttccctctga aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt atggaagatt atgatttcct
      121 gttcaaaatt gttttaattg gcaacgctgg tgtggggaag acgtgcctcg tccgaagatt
      181 cactcagggt cttttccccc caggtcaagg agccacaatt ggagttgatt ttatgattaa
      241 gacagtggag attaatggtg aaaaagtaaa gctacagatc tgggacacag caggtcaaga
      301 gagatttcgg tccattaccc agagttacta ccgaagcgcc aatgccttga tcctcaccta
      361 tgacattacc tgtgaggaat ccttccgttg ccttcctgag tggctgcggg agatagaaca
      421 atatgccagc aacaaggtca tcactgtgtt agtgggcaac aagattgacc tggctgaaag
      481 gagagaggtt tcccagcagc gagctgaaga attctcagaa gctcaggaca tgtattatct
      541 ggagacctca gccaaggaat ctgataatgt ggagaaactc ttccttgact tagcatgccg
      601 actcatcagt gaagccagac agaacacact tgtgaacaat gtatcctgat gacgaagctt
      661 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      721 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      781 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      841 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagc
//
