LOCUS       sequence-31526-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB3D
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      715..762
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tgactatatg ttcaaactgc tactgatagg
      121 caacagcagt gtgggcaaga cttccttcct gttccgatac gcggacgact ccttcactcc
      181 cgccttcgtc agtactgtgg gcatcgattt caaggtcaag accgtctacc gccatgacaa
      241 gaggatcaag ctgcagatct gggacacagc gggccaggag cgctaccgca ccatcaccac
      301 ggcctactac cggggagcca tgggcttcct gctcatgtat gacatcgcca atcaggaatc
      361 ctttgccgct gtgcaggact gggccacgca aatcaagacc tactcctggg acaacgccca
      421 ggtcatcctg gtggggaaca agtgtgacct ggaggacgaa cgtgttgtgc ctgctgagga
      481 tggccggagg ctcgccgacg accttggttt cgagttcttt gaagccagtg ccaaggagaa
      541 catcaatgtg aagcaggtct tcgagcgcct ggtggatgtc atctgcgaga agatgaacga
      601 gtgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      781 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg gggtgtggtg
      841 gttacgcgca gcgtgac
//
