LOCUS       sequence-31527-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RBKS
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tccctctgaa atattttgtt taactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt tggcaagagg aggtggcggc
      121 ggtggtagtg gtgggctcct gcatgaccga cctggtcagt cttacttctc gtttgccaaa
      181 aactggagaa accatccatg gacataagtt ttttattggc tttggaggga aaggtgccaa
      241 ccagtgtgtc caagctgctc ggcttggagc aatgacgtcc atggtgtgta aggttggcaa
      301 agattctttt ggcaatgatt atatagaaaa cttaaaacag aatgatattt ctacagaatt
      361 tacatatcag actaaagatg ctgctacagg aactgcttct ataattgtca ataatgaagg
      421 ccagaatatc attgtcatag tggctggagc aaatttactt ttgaatacgg aggatctgag
      481 ggcagcagcc aatgtcatta gcagagccaa agtcatggtc tgccagctcg aaataactcc
      541 agcaacttct ttggaagccc taacaatggc ccgcaggagt ggagtgaaaa ccttgttcaa
      601 tccagcccct gccattgctg acctggatcc ccagttctac accctctcag atgtgttctg
      661 ctgcaatgaa agtgaggctg agattttaac tggcctcacg gtgggcagcg ctgcagatgc
      721 tggggaggct gcattagtgc tcttgaaaag gggctgccag gtggtaatca ttaccttagg
      781 ggctgaagga tgtgtggtgc tgtcacagac agaacctgag ccaaagcaca ttcccacaga
      841 gaaagtcaag gctgtggata ccacgggtgc tggtgacagc tttngtggga gctctggcct
      901 tctacctggc ttactatcca aatctgt
//
