LOCUS       sequence-31528-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB27B
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             706..723
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(776..794)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      790..837
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccctctgaa atattttgtt taactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt ggagactatg attatctgat
      121 caaactcctg gccctcgggg attcaggggt ggggaagaca acatttcttt atagatacac
      181 agataataaa ttcaatccca aattcatcac tacagtagga atagactttc gggaaaaacg
      241 tgtggtttat aatgcacaag gaccgaatgg atcttcaggg aaagcattta aagtgcatct
      301 tcagctttgg gacactgcgg gacaagagcg gttccggagt ctcaccactg catttttcag
      361 agacgccatg ggcttcttat taatgtttga cctcaccagt caacagagct tcttaaatgt
      421 cagaaactgg atgagccaac tgcaagcaaa tgcttattgt gaaaatccag atatagtatt
      481 aattggcaac aaggcagacc taccagatca gagggaagtc aatgaacggc aagctctgga
      541 actggctgac aaatatggca taccatattt tgaaacaagt gcagcaactg gacagaatgt
      601 ggagaaagct gtagaaaccc ttttggactt aatcatgaag cgaatggaac agtgtgtgga
      661 gaagacacaa atcccttgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac
      721 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      781 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      841 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgta
//
