LOCUS       sequence-315284-        4071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in CHO cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG
  TITLE     Improved elongation factor-1 alpha-based vectors for stable 
            high-level expression of heterologous proteins in Chinese hamster 
            ovary cells.
  JOURNAL   BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.
  PUBMED    24929670
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-06-14"; volume: "14:56. doi"; pages: " 
            10.1186/1472-6750-14-56"
COMMENT     Sequence Label: p1.1
FEATURES             Location/Qualifiers
     source          1..4071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacgttgtgc atagaaacag atgcaggcaa aacatccaca catataaaac aaaaaattaa
       61 aaccaataaa actcctaaac ttttggtctt tcttgaatct tcaatccctc aggttatgaa
      121 ataatcattt atgcagtcaa aaatttgcca ttcttgttgc caggtgtggt gatgattcgg
      181 ggaagcagaa gcaggcagat ctctgtgaat gaggccagcc tggtctacaa agtgagtccc
      241 aggacagtca ggcctgttac acagagaaac cttgaaaaaa aaaagataat atgtactgtt
      301 gtattacccc aatatataag gctaaaccat tagaagcaca acactgttaa gtacggaaaa
      361 taatatctag tgtggtacag ttactactac tataatacac taatatagct gtgggaaact
      421 agttccaaag atgaattact aaccagtgtt tccaaggaaa taaatgaaag cagagagatt
      481 agttctattg ctagtgtttc attttcgtat atttcttaca atttctcttg ttacaaatag
      541 gcactagggt atcaagataa ttttaacgac tggctgagaa ccctagaaaa tctctgtgaa
      601 aaagggattt gtgaaatgag agagggtaat gtggccatta tagaaaaggc ttttgtgtgc
      661 cttgcatgca tagaccctgt gtttgatctc ttaacaccct ccttgaccag aaaaagcttc
      721 tgtggataga aaatgattag ttatatatac ttttagggaa acgtagttct ggattctttg
      781 gttacaatta acagaattaa gtgcaaacaa agccagaaac ctcctgataa atgagaaaac
      841 ctgcttgtag aaggttgtaa ggctctgtaa tataggaatt aggagaaaag aaacctgtgt
      901 ggtggggcac gtctgtaatc ccagcattgg gaagtagagg tagaagatta gaaatcaaag
      961 gccagcctca gcaacacagt gagtttgagg ccaccctgaa ctacatcagg ttctgtctcc
     1021 tttctttttt ttttttttct tttctttttt tggtttctct gtgtagtttt ggagcctatc
     1081 ctggcactag ctctgaagag caggctggcc tcgaactcag agatcagcca gcctctgctg
     1141 ggattaaagg tatgcaccac caacgcccca ggttttgtct caaacaaaca aaaataacat
     1201 caggaggtgg tgagagggct cagtggtcac aggcattctc tgcaaagcct gactctgagt
     1261 tggatccttt agagctacat ggttgaggga agagaactga ctcctggaag gtgtcctctg
     1321 gtccccacac atagctatac acagcatgtg cattcacaca cactaaataa tgctattttt
     1381 aaaaaaatta aaaacaacaa cagtttgggt tgtgaaaact agaactagat aataggtaag
     1441 aatcaagtat catgtaaatt tgctttcaac tcatcccaaa atttgtttta tatttcagtt
     1501 tttttccttc ctagcttgac tgtggagtct tgtccggaag caaatagttc ctttgcagat
     1561 cccacatgtg gacaccggac agtaggtcct caaatgctcc ttattaggtt ggttcaataa
     1621 tatcaattgt ttgttactag gcagtgatgt tgtacatctg gaggagatct cttgagccca
     1681 taatcaggtt attaggaata aatactctaa ggctaaaaat gtagcttagt gataagagtg
     1741 cttgcctggt gtgctgagac cctcggttcc atctccacaa ccccatattc cattacaaaa
     1801 taccttttca ccgtccctag cattaagaaa caaaacaaca aagaagtttt tctttcttct
     1861 gagatcctgc ccggagaggc atttaaaact ggccagggcc aaaaaaaaaa aaaaaaaaag
     1921 aaaaaaaaga aaagaaaaca ggctagggcc ggcatggtgg cgcacgcctt taatcccagc
     1981 acgcaggagg cagaggcagg gcggatctct gtgagtttga ggtcagcctg gtctacctag
     2041 tgagtttcag ggcacccagg gctaaagaga ctgtctcaaa aacaaaacag ccacacaatc
     2101 agaaccacag caaaacgcag ttatgatcct tggaactgta ggaatgacaa gcatttaaat
     2161 aataggacga gccatttttg agaagctctg atttcacaag tgtcagggat gggctctggg
     2221 cgagtaagat tgctaatgct ggcctctaaa tgagaccacg tggagttgat tagattcttt
     2281 tcatgttcct cgtgctctat caaataactg tacccaaata cacacacaca cacacacaca
     2341 caatgcgcgc acacacaaaa tcctttttta gcttaagaag cccagaatca gaagtaaagc
     2401 taactgtggg acttaagtat tattctgaac ggaactccca gggcgtgaag cgcgcttcag
     2461 gcttccagag aagcagctgg cgctggatgg aatgaaccaa gaggccagca caggggcaga
     2521 tccgtcgagc tctcggccac cgagctgagc ccttaggttc tggggctggg aagggtccct
     2581 aggattgtgc acctctcccg cgggggacaa gcagggggat ggcggggctg acgtcgggag
     2641 gtggcctcca cgggaaggga cacccggatc tcgacacagc cttggcagtg gagtcaggaa
     2701 gggtagggac agattctgga cgccctcttg gccagctcct caccgcccca cccccgactg
     2761 gagccgagag taattcatac aaaaggaggg atcgccttcg cccctgggaa tcccagggac
     2821 cgtcgctaaa ttctggccgg cctcccagcc cggaaccgct gtgcccgccc agcgcggcgg
     2881 gaggagcctg cgcctagggc ggatcgcggg tcggcgggag agcacaagcc cacagtcccc
     2941 ggcggtgggg gaggggcgcg ctgagcgggg gcccgggagc cagcgcgggg caaactggga
     3001 aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtataaaag
     3061 tgcggtagtc gcgttggacg ttctttttcg caacgggttt gccgtcagaa cgcaggtgag
     3121 tggcgggtgt ggcctccgcg ggcccgggct ccctcctttg agcggggtcg gaccgccgtg
     3181 cgggtgtcgt cggccgggct tctctgcgag cgttcccgcc ctggatggcg ggctgtgcgg
     3241 gagggcgagg gggggaggcc tggcggcggc cccggagcct cgcctcgtgt cgggcgtgag
     3301 gcctagcgtg gcttccgccc cgccgcgtgc caccgcggcc gcgctttgct gtctgcccgg
     3361 ctgccctcga ttgcctgccc gcggcccggg ccaacaaagg gagggcgtgg agctggctgg
     3421 tagggagccc cgtagtccgc atgtcgggca gggagagcgg cagcagtcgg gggggggacc
     3481 gggcccgccc gtcccgcagc acatgtccga cgccgcctgg acgggtagcg gcctgtgtcc
     3541 tgataaggcg gccgggcggt gggttttaga tgccgggttc aggtggcccc gggtcccggc
     3601 ccggtctggc cagtaccccg tagtggctta gctccgagga gggcgaggcc cgcccgcccg
     3661 gcaccagttg cgtgcgcgga aagatggccg ctcccgggcc ctgtagcaag gagctcaaaa
     3721 tggaggacgc ggcagcccgg cggagcgggg cgggtgagtc acccacacaa aggaagaggg
     3781 ccttgcccct cgccggccgc tgcttcctgt gaccccgtgg tgtaccggcc gcacttcagt
     3841 caccccgggc gctctttcgg agcaccgctg gcctccgctg ggggagggga tctgtctaat
     3901 ggcgttggag tttgctcaca tttggtgggt ggagactgta gccaggccag cctggccatg
     3961 aaagtaattc ttggaatttg cccattttga gtttggagcg aagctgattg acaaagctgc
     4021 ttagccgttc aaaggtattc ttcgaacttt ttttttaagg tgttgtgaaa a
//
