LOCUS       sequence-31530-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CKMT2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gagtaattcc ctctgaatat tttgtttaac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggttccgcgt ggtagtacca ccgggtacct
      121 gctgaaccgg cagaaagtgt gtgccgaggt ccgggagcag cctaggctat ttcctccaag
      181 cgcagactac ccagacctgc gcaagcacaa caactgcatg gccgagtgcc tcacccccgc
      241 catttatgcc aagcttcgca acaaggtgac acccaacggc tacacgctgg accagtgcat
      301 ccagactgga gtggacaacc ctggccaccc cttcataaag actgtgggca tggtggctgg
      361 tgacgaggag tcctatgagg tgtttgctga cctttttgac cccgtcatca aactaagaca
      421 caacggctat gaccccaggg tgatgaagca cacaacggat ctggatgcat caaagatcac
      481 ccaagggcag ttcgacgagc attacgtgct gtcttctcgg gtgcgcactg gccgcagcat
      541 ccgtgggctg agcctgcctc cagcctgcac ccgggccgag cgaagggagg tagagaacgt
      601 ggccatcact gccctggagg gcctcaaggg ggacctggct ggccgctact acaagctgtc
      661 cgagatgacg gagcaggacc agcagcggct catcgatgac cactttctgt ttgataagcc
      721 agtgtcccct ttattaacat gtgctgggat ggcccgtgac tggccagatg ccaggggaat
      781 ctggcataat tatgataaga catttctcat ctggataaat gaggaggatc acaccagggt
      841 aatctcaat
//
