LOCUS       sequence-315308-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing dCas9 and sgRNA containg MTSa targeting telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK
  TITLE     A CRISPR/molecular beacon hybrid system for live-cell genomic 
            imaging.
  JOURNAL   Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304.
  PUBMED    29718399
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-07-27"; volume: "46(13):e80. doi"; pages: "
            10.1093/nar/gky304"
COMMENT     Sequence Label: SL2-sgTelo-MTSa/EGFP/pdCas9-C1
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    227..307
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      309..318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(315..317,318..320,321..1034)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(360..381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(621..640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     968..989
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1157..1278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1292..1540
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1292..1312
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1463..1482
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        1794..2097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2098..2301
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2341..2361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2362..2385,2386..2388,2389..4875,4876..4878,
                     4879..6462)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6472..6492
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6689..6810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6817..7272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6954..6975)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7166..7185
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7299..7403
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7405..7762
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7405..7596
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7428..7448)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7613..7748
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7797..8591
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7797..8591
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7851..7870)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8461..8480
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8779..8798)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8823..8870
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9199..9787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9688..9707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9913..10216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      301 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      361 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      421 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      481 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      541 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      601 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      661 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      721 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      781 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      841 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      901 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      961 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1021 agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     1081 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1141 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1261 actcatcaat gtatcttatt aatagttatt agagggccta tttcccatga ttccttcata
     1321 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     1381 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     1441 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     1501 tttcttggct ttatatatct tgtggaaagg acgaaacacc gttagggtta gggttagggt
     1561 tagtttgaga gctatgctgg aaacagcata gcaagttcaa ataaggctag tccgttatca
     1621 acttggcccc ggagcagaac gacaggagtt gtgtttgtgg acgaagagcc tgcagtctgc
     1681 tccggggcca gtggcaccga gtcggtgctt tttttagaga cggagtcact gccaaccgat
     1741 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     1801 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     1861 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1921 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1981 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2041 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2101 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2161 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2221 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2281 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcc caccatggtg
     2341 cccaaaaaga agaggaaagt ggacaagaag tattctatcg gactggccat cgggactaat
     2401 agcgtcgggt gggccgtgat cactgacgag tacaaggtgc cctctaagaa gttcaaggtg
     2461 ctcgggaaca ccgaccggca ttccatcaag aaaaatctga tcggagctct cctctttgat
     2521 tcaggggaga ccgctgaagc aacccgcctc aagcggactg ctagacggcg gtacaccagg
     2581 aggaagaacc ggatttgtta ccttcaagag atattctcca acgaaatggc aaaggtcgac
     2641 gacagcttct tccataggct ggaagaatca ttcctcgtgg aagaggataa gaagcatgaa
     2701 cggcatccca tcttcggtaa tatcgtcgac gaggtggcct atcacgagaa atacccaacc
     2761 atctaccatc ttcgcaaaaa gctggtggac tcaaccgaca aggcagacct ccggcttatc
     2821 tacctggccc tggcccacat gatcaagttc agaggccact tcctgatcga gggcgacctc
     2881 aatcctgaca atagcgatgt ggataaactg ttcatccagc tggtgcagac ttacaaccag
     2941 ctctttgaag agaaccccat caatgcaagc ggagtcgatg ccaaggccat tctgtcagcc
     3001 cggctgtcaa agagccgcag acttgagaat cttatcgctc agctgccggg tgaaaagaaa
     3061 aatggactgt tcgggaacct gattgctctt tcacttgggc tgactcccaa tttcaagtct
     3121 aatttcgacc tggcagagga tgccaagctg caactgtcca aggacaccta tgatgacgat
     3181 ctcgacaacc tcctggccca gatcggtgac caatacgccg accttttcct tgctgctaag
     3241 aatctttctg acgccatcct gctgtctgac attctccgcg tgaacactga aatcaccaag
     3301 gcccctcttt cagcttcaat gattaagcgg tatgatgagc accaccagga cctgaccctg
     3361 cttaaggcac tcgtccggca gcagcttccg gagaagtaca aggaaatctt ctttgaccag
     3421 tcaaagaatg gatacgccgg ctacatcgac ggaggtgcct cccaagagga attttataag
     3481 tttatcaaac ctatccttga gaagatggac ggcaccgaag agctcctcgt gaaactgaat
     3541 cgggaggatc tgctgcggaa gcagcgcact ttcgacaatg ggagcattcc ccaccagatc
     3601 catcttgggg agcttcacgc catccttcgg cgccaagagg acttctaccc ctttcttaag
     3661 gacaacaggg agaagattga gaaaattctc actttccgca tcccctacta cgtgggaccc
     3721 ctcgccagag gaaatagccg gtttgcttgg atgaccagaa agtcagaaga aactatcact
     3781 ccctggaact tcgaagaggt ggtggacaag ggagccagcg ctcagtcatt catcgaacgg
     3841 atgactaact tcgataagaa cctccccaat gagaaggtcc tgccgaaaca ttccctgctc
     3901 tacgagtact ttaccgtgta caacgagctg accaaggtga aatatgtcac cgaagggatg
     3961 aggaagcccg cattcctgtc aggcgaacaa aagaaggcaa ttgtggacct tctgttcaag
     4021 accaatagaa aggtgaccgt gaagcagctg aaggaggact atttcaagaa aattgaatgc
     4081 ttcgactctg tggagattag cggggtcgaa gatcggttca acgcaagcct gggtacctac
     4141 catgatctgc ttaagatcat caaggacaag gattttctgg acaatgagga gaacgaggac
     4201 atccttgagg acattgtcct gactctcact ctgttcgagg accgggaaat gatcgaggag
     4261 aggcttaaga cctacgccca tctgttcgac gataaagtga tgaagcaact taaacggaga
     4321 agatataccg gatggggacg ccttagccgc aaactcatca acggaatccg ggacaaacag
     4381 agcggaaaga ccattcttga tttccttaag agcgacggat tcgctaatcg caacttcatg
     4441 caacttatcc atgatgattc cctgaccttt aaggaggaca tccagaaggc ccaagtgtct
     4501 ggacaaggtg actcactgca cgagcatatc gcaaatctgg ctggttcacc cgctattaag
     4561 aagggtattc tccagaccgt gaaagtcgtg gacgagctgg tcaaggtgat gggtcgccat
     4621 aaaccagaga acattgtcat cgagatggcc agggaaaacc agactaccca gaagggacag
     4681 aagaacagca gggagcggat gaaaagaatt gaggaaggga ttaaggagct cgggtcacag
     4741 atccttaaag agcacccggt ggaaaacacc cagcttcaga atgagaagct ctatctgtac
     4801 taccttcaaa atggacgcga tatgtatgtg gaccaagagc ttgatatcaa caggctctca
     4861 gactacgacg tggacgccat cgtccctcag agcttcctca aagacgactc aattgacaat
     4921 aaggtgctga ctcgctcaga caagaaccgg ggaaagtcag ataacgtgcc ctcagaggaa
     4981 gtcgtgaaaa agatgaagaa ctattggcgc cagcttctga acgcaaagct gatcactcag
     5041 cggaagttcg acaatctcac taaggctgag aggggcggac tgagcgaact ggacaaagca
     5101 ggattcatta aacggcaact tgtggagact cggcagatta ctaaacatgt cgcccaaatc
     5161 cttgactcac gcatgaatac caagtacgac gaaaacgaca aacttatccg cgaggtgaag
     5221 gtgattaccc tgaagtccaa gctggtcagc gatttcagaa aggactttca attctacaaa
     5281 gtgcgggaga tcaataacta tcatcatgct catgacgcat atctgaatgc cgtggtggga
     5341 accgccctga tcaagaagta cccaaagctg gaaagcgagt tcgtgtacgg agactacaag
     5401 gtctacgacg tgcgcaagat gattgccaaa tctgagcagg agatcggaaa ggccaccgca
     5461 aagtacttct tctacagcaa catcatgaat ttcttcaaga ccgaaatcac ccttgcaaac
     5521 ggtgagatcc ggaagaggcc gctcatcgag actaatgggg agactggcga aatcgtgtgg
     5581 gacaagggca gagatttcgc taccgtgcgc aaagtgcttt ctatgcctca agtgaacatc
     5641 gtgaagaaaa ccgaggtgca aaccggaggc ttttctaagg aatcaatcct ccccaagcgc
     5701 aactccgaca agctcattgc aaggaagaag gattgggacc ctaagaagta cggcggattc
     5761 gattcaccaa ctgtggctta ttctgtcctg gtcgtggcta aggtggaaaa aggaaagtct
     5821 aagaagctca agagcgtgaa ggaactgctg ggtatcacca ttatggagcg cagctccttc
     5881 gagaagaacc caattgactt tctcgaagcc aaaggttaca aggaagtcaa gaaggacctt
     5941 atcatcaagc tcccaaagta tagcctgttc gaactggaga atgggcggaa gcggatgctc
     6001 gcctccgctg gcgaacttca gaagggtaat gagctggctc tcccctccaa gtacgtgaat
     6061 ttcctctacc ttgcaagcca ttacgagaag ctgaagggga gccccgagga caacgagcaa
     6121 aagcaactgt ttgtggagca gcataagcat tatctggacg agatcattga gcagatttcc
     6181 gagttttcta aacgcgtcat tctcgctgat gccaacctcg ataaagtcct tagcgcatac
     6241 aataagcaca gagacaaacc aattcgggag caggctgaga atatcatcca cctgttcacc
     6301 ctcaccaatc ttggtgcccc tgccgcattc aagtacttcg acaccaccat cgaccggaaa
     6361 cgctatacct ccaccaaaga agtgctggac gccaccctca tccaccagag catcaccgga
     6421 ctttacgaaa ctcggattga cctctcacag ctcggagggg atgagggagc tcccaagaaa
     6481 aagcgcaagg taggtagttc cgtgagcaag ggcgaggagc tgttctaaga attctgcagt
     6541 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
     6601 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6661 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     6721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6781 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt
     6841 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     6901 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     6961 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     7021 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     7081 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     7141 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     7201 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     7261 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7321 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7381 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     7441 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7501 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7561 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7621 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7681 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7741 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     7801 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7861 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7921 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     7981 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     8041 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     8101 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     8161 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     8221 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     8281 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     8341 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     8401 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     8461 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     8521 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     8581 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     8641 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8701 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8761 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     8821 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     8881 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     8941 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     9001 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     9061 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9121 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9181 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9241 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9301 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9361 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9421 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9481 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9541 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9601 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9721 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9781 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9841 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     9901 ttagttatta cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     9961 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    10021 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    10081 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    10141 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
    10201 catcgctatt accatg
//
