LOCUS       sequence-31532-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KIF1C-FHA
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             434..451
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(504..522)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      518..565
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(689..708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcctctgaa tattttgttt actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggttccgc gtggtagtac tccccacctg gtgaacctga
      121 acgaagaccc tctgatgtct gagtgtctgc tctaccacat caaagatggc gtcaccaggg
      181 tcggccaagt agatatggac atcaagctga ccggacagtt cattcgggag caacactgtc
      241 tgttccggag catcccccag ccagatggag aagtggtggt cactctggag ccttgtgaag
      301 gagctgagac atatgtgaat gggaagcttg tgacggagcc gctggtgctg aagtcaggga
      361 ataggattgt gatgggcaag aaccacgttt tccgcttcaa ccactgatga cgaagcttgc
      421 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      481 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      541 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      601 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      661 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      721 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      781 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatgggtt cacgtagtgg
      841 gccatcgccc ctgatagac
//
