LOCUS       sequence-315321-        7245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9-nuclear localization, level 0 of MoClo Golden Gate position 
            CDS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Triozzi P, Schmidt HW, Dervinis C, Kirst M, Conde D
  TITLE     Simple, efficient and open-source CRISPR/Cas9 strategy for 
            multi-site genome editing in Populus tremula x alba.
  JOURNAL   Tree Physiol. 2021 May 7. pii: 6270869. doi: 
            10.1093/treephys/tpab066.
  PUBMED    33960379
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tree 
            Physiol. 2021 May 7. pii: 6270869. doi: 10.1093/treephys/tpab066."
COMMENT     Sequence Label: pGEM-T Easy-Cas9-nls-CDS1
FEATURES             Location/Qualifiers
     source          1..7245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(79..126)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(127..4227)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(127..4227)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4252..4272)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(4370..4388)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4371..4388)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4406..4422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4406..4422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4419..4441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4430..4446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4454..4460,4461..4478,4479..4484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4499..4520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4637..4654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4808..5396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4888..4907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5567..6358,6359..6427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6190..6209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6428..6532)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6610..7065)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6747..6768)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6959..6978
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7191..7213
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7205..7222
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7206..7222
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7229..7245,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(7229..7245,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tattggtctc taagcttact ttttcttttt tgcctggccg gcctttttcg tggccgccgg
      121 ccttttgtcg cctcccagct gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct
      181 ctggtggatc agggtggcgt ccagcacctc tttggtgctg gtgtacctct tccggtcgat
      241 ggtggtgtca aagtacttga aggcggcagg ggctcccaga ttggtcaggg taaacaggtg
      301 gatgatattc tcggcctgct ctctgatggg cttatcccgg tgcttgttgt aggcggacag
      361 cactttgtcc agattagcgt cggccaggat cactctcttg gagaactcgc tgatctgctc
      421 gatgatctcg tccaggtagt gcttgtgctg ttccacaaac agctgtttct gctcattatc
      481 ctcgggggag cccttcagct tctcatagtg gctggccagg tacaggaagt tcacatattt
      541 ggagggcagg gccagttcgt ttcccttctg cagttcgccg gcagaggcca gcattctctt
      601 ccggccgttt tccagctcga acagggagta cttaggcagc ttgatgatca ggtccttttt
      661 cacttctttg tagcccttgg cttccagaaa gtcgatggga ttcttctcga agctgcttct
      721 ttccatgatg gtgatcccca gcagctcttt cacactcttc agtttcttgg acttgccctt
      781 ttccactttg gccaccacca gcacagaata ggccacggtg gggctgtcga agccgccgta
      841 cttcttaggg tcccagtcct tctttctggc gatcagctta tcgctgttcc tcttgggcag
      901 gatagactct ttgctgaagc cgcctgtctg cacctcggtc tttttcacga tattcacttg
      961 gggcatgctc agcactttcc gcacggtggc aaaatcccgg cccttatccc acacgatctc
     1021 cccggtttcg ccgtttgtct cgatcagagg ccgcttccgg atctcgccgt tggccagggt
     1081 aatctcggtc ttgaaaaagt tcatgatgtt gctgtagaag aagtacttgg cggtagcctt
     1141 gccgatttcc tgctcgctct tggcgatcat cttccgcacg tcgtacacct tgtagtcgcc
     1201 gtacacgaac tcgctttcca gcttagggta ctttttgatc agggcggttc ccacgacggc
     1261 gttcaggtag gcgtcgtggg cgtggtggta gttgttgatc tcgcgcactt tgtaaaactg
     1321 gaaatccttc cggaaatcgg acaccagctt ggacttcagg gtgatcactt tcacttcccg
     1381 gatcagcttg tcattctcgt cgtacttagt gttcatccgg gagtccagga tctgtgccac
     1441 gtgctttgtg atctgccggg tttccaccag ctgtctcttg atgaagccgg ccttatccag
     1501 ttcgctcagg ccgcctctct cggccttggt cagattgtcg aactttctct gggtaatcag
     1561 cttggcgttc agcagctgcc gccagtagtt cttcatcttc ttcacgacct cttcggaggg
     1621 cacgttgtcg ctcttgcccc ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga
     1681 gtcgtccttc agaaagctct gaggcacgat atggtccaca tcgtagtcgg acagccggtt
     1741 gatgtccagt tcctggtcca cgtacatatc ccgcccattc tgcaggtagt acaggtacag
     1801 cttctcgttc tgcagctggg tgttttccac ggggtgttct ttcaggatct ggctgcccag
     1861 ctctttgatg ccctcttcga tccgcttcat tctctcgcgg ctgttcttct gtcccttctg
     1921 ggtggtctgg ttctctctgg ccatttcgat cacgatgttc tcgggcttgt gccggcccat
     1981 cactttcacg agctcgtcca ccaccttcac tgtctgcagg atgcccttct taatggcggg
     2041 gctgccggcc agattggcaa tgtgctcgtg caggctatcg ccctggccgg acacctgggc
     2101 tttctggatg tcctctttaa aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct
     2161 gttggcgaag ccgtcggact tcaggaaatc caggattgtc ttgccggact gcttgtcccg
     2221 gatgccgttg atcagcttcc ggctcagcct gccccagccg gtgtatctcc gccgcttcag
     2281 ctgcttcatc actttgtcgt cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat
     2341 ctctctgtcc tcaaacagtg tcagggtcag cacgatatct tccagaatgt cctcgttttc
     2401 ctcattgtcc aggaagtcct tgtccttgat aattttcagc agatcgtggt atgtgcccag
     2461 ggaggcgttg aaccgatctt ccacgccgga gatttccacg gagtcgaagc actcgatttt
     2521 cttgaagtag tcctctttca gctgcttcac ggtcactttc cggttggtct tgaacagcag
     2581 gtccacgatg gcctttttct gctcgccgct caggaaggcg ggctttctca ttccctcggt
     2641 cacgtatttc actttggtca gctcgttata cacggtgaag tactcgtaca gcaggctgtg
     2701 cttgggcagc accttctcgt tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa
     2761 gctctgggcg gaagcgccct tgtccaccac ttcctcgaag ttccaggggg tgatggtttc
     2821 ctcgctcttt ctggtcatcc aggcgaatct gctgtttccc ctggccagag ggcccacgta
     2881 gtaggggatg cggaaggtca ggatcttctc gatcttttcc cggttgtcct tcaggaatgg
     2941 gtaaaaatct tcctgccgcc gcagaatggc gtgcagctct cccaggtgga tctggtgggg
     3001 gatgctgccg ttgtcgaagg tccgctgctt ccgcagcagg tcctctctgt tcagcttcac
     3061 gagcagttcc tcggtgccgt ccatcttttc caggatgggc ttgatgaact tgtagaactc
     3121 ttcctggctg gctccgccgt caatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa
     3181 aatctctttg tacttctcag gcagctgctg ccgcacgaga gctttcagca gggtcaggtc
     3241 ctggtggtgc tcgtcgtatc tcttgatcat agaggcgctc aggggggcct tggtgatctc
     3301 ggtgttcact ctcaggatgt cgctcagcag gatggcgtcg gacaggttct tggcggccag
     3361 aaacaggtcg gcgtactggt cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta
     3421 ggtgtccttg ctcagctgca gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt
     3481 gggggtcagg cccaggctca gggcaatcag gtttccgaac aggccattct tcttctcgcc
     3541 gggcagctgg gcgatcagat tttccagccg tctgctcttg ctcagtctgg cagacaggat
     3601 ggccttggcg tccacgccgc tggcgttgat ggggttttcc tcgaacagct ggttgtaggt
     3661 ctgcaccagc tggatgaaca gcttgtccac gtcgctgttg tcggggttca ggtcgccctc
     3721 gatcaggaag tggccccgga acttgatcat gtgggccagg gccagataga tcagccgcag
     3781 gtcggccttg tcggtgctgt ccaccagttt ctttctcagg tggtagatgg tggggtactt
     3841 ctcgtggtag gccacctcgt ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt
     3901 atcctcttcc accaggaagg actcttccag tctgtggaag aagctgtcgt ccaccttggc
     3961 catctcgttg ctgaagatct cttgcagata gcagatccgg ttcttccgtc tggtgtatct
     4021 tcttctggcg gttctcttca gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag
     4081 ggctccgatc aggttcttct tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt
     4141 cttgctgggc accttgtact cgtcggtgat cacggcccag cccacagagt tggtgccgat
     4201 gtccaggccg atgctgtact tcttgtcggc tgctgggact ccgtggatac cgaccttccg
     4261 cttcttcttt ggggccattt gagaccatta atcactagtg aattcgcggc cgcctgcagg
     4321 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
     4381 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4501 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4561 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5221 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5521 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5581 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5641 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5701 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5761 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5821 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5881 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5941 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6001 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6061 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6541 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6601 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     6661 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6721 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     6781 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     6841 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6901 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6961 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7021 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     7081 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     7141 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     7201 acgttgtaaa acgacggcca gtgaattgta atacgactca ctata
//
