LOCUS       sequence-315330-       14569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble human PACE/furin protease expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Kovnir SV, Orlova NA, Shakhparonov MI, Skryabin KG, Gabibov AG, 
            Vorobiev II
  TITLE     A Highly Productive CHO Cell Line Secreting Human Blood Clotting 
            Factor IX.
  JOURNAL   Acta Naturae. 2018 Jan-Mar;10(1):51-65.
  PUBMED    29713519
REFERENCE   2  (bases 1 to 14569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Naturae. 2018 Jan-Mar"; volume: "10(1)"; pages: "51-65"
COMMENT     Sequence Label: p1.2-Hyg-Fur
FEATURES             Location/Qualifiers
     source          1..14569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..55)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(209..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(289..308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      6454..6463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7757..7766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(12897..12917)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12902..13231
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13082..13217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13144..13163
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             13280..14305
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             13280..14305
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    14435..14556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14472..14491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14526..14545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccgtcgacct ctaggcgcgc catggctctt ccgcttcctc gctcactgac tcgctgcgct
       61 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      121 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      181 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      241 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      301 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      361 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      421 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      481 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      541 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      601 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      661 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      721 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctgcatc tcatcacggt cacgcatggc
     1981 tgcgcccgcg cagcgccccc cgaccccccc ccgatccccg gaacgccagc cgccatccac
     2041 cgcccgcagc ccccggagcc cgcggacccc gaccccccgc cgctcgcccg ccgttgcgcc
     2101 gctctctgcg ggggggctag ggggccgcgg ggggaaggcc acgccccctc cactttttcc
     2161 aggaatgcgc ggcatgaccc gcccccccca tgacccgcca acaccccccc gatgcccccc
     2221 gggggtcttt cctggggggc ctttgtcagg gttgcctgtg tcacccacgg gccccttggc
     2281 cacgcttgcc tgggtcaccc cggagccccg ggtcacgttt gccggggtca gccctggggc
     2341 gcttcttcac gctccattgc catgcagaat tccacgttgt gcatagaaac agatgcaggc
     2401 aaaacatcca cacatataaa acaaaaaatt aaaaccaata aaactcctaa acttttggtc
     2461 tttcttgaat cttcaatccc tcaggttatg aaataatcat ttatgcagtc aaaaatttgc
     2521 cattcttgtt gccaggtgtg gtgatgattc ggggaagcag aagcaggcag atctctgtga
     2581 atgaggccag cctggtctac aaagtgagtc ccaggacagt caggcctgtt acacagagaa
     2641 accttgaaaa aaaaaagata atatgtactg ttgtattacc ccaatatata aggctaaacc
     2701 attagaagca caacactgtt aagtacggaa aataatatct agtgtggtac agttactact
     2761 actataatac actaatatag ctgtgggaaa ctagttccaa agatgaatta ctaaccagtg
     2821 tttccaagga aataaatgaa agcagagaga ttagttctat tgctagtgtt tcattttcgt
     2881 atatttctta caatttctct tgttacaaat aggcactagg gtatcaagat aattttaacg
     2941 actggctgag aaccctagaa aatctctgtg aaaaagggat ttgtgaaatg agagagggta
     3001 atgtggccat tatagaaaag gcttttgtgt gccttgcatg catagaccct gtgtttgatc
     3061 tcttaacacc ctccttgacc agaaaaagct tctgtggata gaaaatgatt agttatatat
     3121 acttttaggg aaacgtagtt ctggattctt tggttacaat taacagaatt aagtgcaaac
     3181 aaagccagaa acctcctgat aaatgagaaa acctgcttgt agaaggttgt aaggctctgt
     3241 aatataggaa ttaggagaaa agaaacctgt gtggtggggc acgtctgtaa tcccagcatt
     3301 gggaagtaga ggtagaagat tagaaatcaa aggccagcct cagcaacaca gtgagtttga
     3361 ggccaccctg aactacatca ggttctgtct cctttctttt tttttttttt cttttctttt
     3421 tttggtttct ctgtgtagtt ttggagccta tcctggcact agctctgaag agcaggctgg
     3481 cctcgaactc agagatcagc cagcctctgc tgggattaaa ggtatgcacc accaacgccc
     3541 caggttttgt ctcaaacaaa caaaaataac atcaggaggt ggtgagaggg ctcagtggtc
     3601 acaggcattc tctgcaaagc ctgactctga gttggatcct ttagagctac atggttgagg
     3661 gaagagaact gactcctgga aggtgtcctc tggtccccac acatagctat acacagcatg
     3721 tgcattcaca cacactaaat aatgctattt ttaaaaaaat taaaaacaac aacagtttgg
     3781 gttgtgaaaa ctagaactag ataataggta agaatcaagt atcatgtaaa tttgctttca
     3841 actcatccca aaatttgttt tatatttcag tttttttcct tcctagcttg actgtggagt
     3901 cttgtccgga agcaaatagt tcctttgcag atcccacatg tggacaccgg acagtaggtc
     3961 ctcaaatgct ccttattagg ttggttcaat aatatcaatt gtttgttact aggcagtgat
     4021 gttgtacatc tggaggagat ctcttgagcc cataatcagg ttattaggaa taaatactct
     4081 aaggctaaaa atgtagctta gtgataagag tgcttgcctg gtgtgctgag accctcggtt
     4141 ccatctccac aaccccatat tccattacaa aatacctttt caccgtccct agcattaaga
     4201 aacaaaacaa caaagaagtt tttctttctt ctgagatcct gcccggagag gcatttaaaa
     4261 ctggccaggg ccaaaaaaaa aaaaaaaaaa agaaaaaaaa gaaaagaaaa caggctaggg
     4321 ccggcatggt ggcgcacgcc tttaatccca gcacgcagga ggcagaggca gggcggatct
     4381 ctgtgagttt gaggtcagcc tggtctacct agtgagtttc agggcaccca gggctaaaga
     4441 gactgtctca aaaacaaaac agccacacaa tcagaaccac agcaaaacgc agttatgatc
     4501 cttggaactg taggaatgac aagcatttaa ataataggac gagccatttt tgagaagctc
     4561 tgatttcaca agtgtcaggg atgggctctg ggcgagtaag attgctaatg ctggcctcta
     4621 aatgagacca cgtggagttg attagattct tttcatgttc ctcgtgctct atcaaataac
     4681 tgtacccaaa tacacacaca cacacacaca cacaatgcgc gcacacacaa aatccttttt
     4741 tagcttaaga agcccagaat cagaagtaaa gctaactgtg ggacttaagt attattctga
     4801 acggaactcc cagggcgtga agcgcgcttc aggcttccag agaagcagct ggcgctggat
     4861 ggaatgaacc aagaggccag cacaggggca gatccgtcga gctctcggcc accgagctga
     4921 gcccttaggt tctggggctg ggaagggtcc ctaggattgt gcacctctcc cgcgggggac
     4981 aagcaggggg atggcggggc tgacgtcggg aggtggcctc cacgggaagg gacacccgga
     5041 tctcgacaca gccttggcag tggagtcagg aagggtaggg acagattctg gacgccctct
     5101 tggccagctc ctcaccgccc cacccccgac tggagccgag agtaattcat acaaaaggag
     5161 ggatcgcctt cgcccctggg aatcccaggg accgtcgcta aattctggcc ggcctcccag
     5221 cccggaaccg ctgtgcccgc ccagcgcggc gggaggagcc tgcgcctagg gcggatcgcg
     5281 ggtcggcggg agagcacaag cccacagtcc ccggcggtgg gggaggggcg cgctgagcgg
     5341 gggcccggga gccagcgcgg ggcaaactgg gaaagtggtg tcgtgtgctg gctccgccct
     5401 cttcccgagg gtgggggaga acggtataaa agtgcggtag tcgcgttgga cgttcttttt
     5461 cgcaacgggt ttgccgtcag aacgcaggtg agtggcgggt gtggcctccg cgggcccggg
     5521 ctccctcctt tgagcggggt cggaccgccg tgcgggtgtc gtcggccggg cttctctgcg
     5581 agcgttcccg ccctggatgg cgggctgtgc gggagggcga gggggggagg cctggcggcg
     5641 gccccggagc ctcgcctcgt gtcgggcgtg aggcctagcg tggcttccgc cccgccgcgt
     5701 gccaccgcgg ccgcgctttg ctgtctgccc ggctgccctc gattgcctgc ccgcggcccg
     5761 ggccaacaaa gggagggcgt ggagctggct ggtagggagc cccgtagtcc gcatgtcggg
     5821 cagggagagc ggcagcagtc ggggggggga ccgggcccgc ccgtcccgca gcacatgtcc
     5881 gacgccgcct ggacgggtag cggcctgtgt cctgataagg cggccgggcg gtgggtttta
     5941 gatgccgggt tcaggtggcc ccgggtcccg gcccggtctg gccagtaccc cgtagtggct
     6001 tagctccgag gagggcgagg cccgcccgcc cggcaccagt tgcgtgcgcg gaaagatggc
     6061 cgctcccggg ccctgtagca aggagctcaa aatggaggac gcggcagccc ggcggagcgg
     6121 ggcgggtgag tcacccacac aaaggaagag ggccttgccc ctcgccggcc gctgcttcct
     6181 gtgaccccgt ggtgtaccgg ccgcacttca gtcaccccgg gcgctctttc ggagcaccgc
     6241 tggcctccgc tgggggaggg gatctgtcta atggcgttgg agtttgctca catttggtgg
     6301 gtggagactg tagccaggcc agcctggcca tgaaagtaat tcttggaatt tgcccatttt
     6361 gagtttggag cgaagctgat tgacaaagct gcttagccgt tcaaaggtat tcttcgaact
     6421 ttttttttaa ggtgttgtga aaacctcgag gccgccacca tggagctgag gccctggttg
     6481 ctatgggtgg tagcagcaac aggaaccttg gtcctgctag cagctgatgc tcagggccag
     6541 aaggtcttca ccaacacgtg ggctgtgcgc atccctggag gcccagcggt ggccaacagt
     6601 gtggcacgga agcatgggtt cctcaacctg ggccagatct tcggggacta ttaccacttc
     6661 tggcatcgag gagtgacgaa gcggtccctg tcgcctcacc gcccgcggca cagccggctg
     6721 cagagggagc ctcaagtaca gtggctggaa cagcaggtgg caaagcgacg gactaaacgg
     6781 gacgtgtacc aggagcccac agaccccaag tttcctcagc agtggtacct gtctggtgtc
     6841 actcagcggg acctgaatgt gaaggcggcc tgggcgcagg gctacacagg gcacggcatt
     6901 gtggtctcca ttctggacga tggcatcgag aagaaccacc cggacttggc aggcaattat
     6961 gatcctgggg ccagttttga tgtcaatgac caggaccctg acccccagcc tcggtacaca
     7021 cagatgaatg acaacaggca cggcacacgg tgtgcggggg aagtggctgc ggtggccaac
     7081 aacggtgtct gtggtgtagg tgtggcctac aacgcccgca ttggaggggt gcgcatgctg
     7141 gatggcgagg tgacagatgc agtggaggca cgctcgctgg gcctgaaccc caaccacatc
     7201 cacatctaca gtgccagctg gggccccgag gatgacggca agacagtgga tgggccagcc
     7261 cgcctcgccg aggaggcctt cttccgtggg gttagccagg gccgaggggg gctgggctcc
     7321 atctttgtct gggcctcggg gaacgggggc cgggaacatg acagctgcaa ctgcgacggc
     7381 tacaccaaca gtatctacac gctgtccatc agcagcgcca cgcagtttgg caacgtgccg
     7441 tggtacagcg aggcctgctc gtccacactg gccacgacct acagcagtgg caaccagaat
     7501 gagaagcaga tcgtgacgac tgacttgcgg cagaagtgca cggagtctca cacgggcacc
     7561 tcagcctctg cccccttagc agccggcatc attgctctca ccctggaggc caataagaac
     7621 ctcacatggc gggacatgca acacctggtg gtacagacct cgaagccagc ccacctcaat
     7681 gccaacgact gggccaccaa tggtgtgggc cggaaagtga gccactcata tggctacggg
     7741 cttttggacg caggcgccat ggtggccctg gcccagaatt ggaccacagt ggccccccag
     7801 cggaagtgca tcatcgacat cctcaccgag cccaaagaca tcgggaaacg gctcgaggtg
     7861 cggaagaccg tgaccgcgtg cctgggcgag cccaaccaca tcactcggct ggagcacgct
     7921 caggcgcggc tcaccctgtc ctataatcgc cgtggcgacc tggccatcca cctggtcagc
     7981 cccatgggca cccgctccac cctgctggca gccaggccac atgactactc cgcagatggg
     8041 tttaatgact gggccttcat gacaactcat tcctgggatg aggatccctc tggcgagtgg
     8101 gtcctagaga ttgaaaacac cagcgaagcc aacaactatg ggacgctgac caagttcacc
     8161 ctcgtactct atggcaccgc ccctgagggg ctgcccgtac ctccagaaag cagtggctgc
     8221 aagaccctca cgtccagtca ggcctgtgtg gtgtgcgagg aaggcttctc cctgcaccag
     8281 aagagctgtg tccagcactg ccctccaggc ttcgcccccc aagtcctcga tacgcactat
     8341 agcaccgaga atgacgtgga gaccatccgg gccagcgtct gcgccccctg ccacgcctca
     8401 tgtgccacat gccaggggcc ggccctgaca gactgcctca gctgccccag ccacgcctcc
     8461 ttggaccctg tggagcagac ttgctcccgg caaagccaga gcagccgaga gtccccgcca
     8521 cagcagcagc cacctcggct gcccccggag gtggaggcgg ggcaacggct gcgggcaggg
     8581 ctgctgccct cacacctgcc tgagtgatag tctagcatat tacccctaac acctgccacc
     8641 ccagtcttaa tcagtggtgg aagaacggtc tcagaactgt ttgtctcaat tggccattta
     8701 agtttaatag tgaaagactg gttaatgata acaatgcatc ggaaaacctt caggaggaaa
     8761 ggagaatgtt ttgtggaaca tttttgtgtg tgtggcagtt ttaagttatt agttttcaaa
     8821 atcagtactt tttaatggaa acaacttgac caaaaatctg tcacagaatt ttgagaccca
     8881 ttaaaataca agtttaatga gaagtctgtc tctgttaatg ctgaagtcat tactaagtgc
     8941 ttagcttagc aaggtatgtg gatgcccatt tgtgttccaa gggattggac tgttcatcag
     9001 gacccagagc tgagtttcaa gggctcaaga gatggcttat tacctgtggg tgtcttgaag
     9061 gttctggttg ggacaaatta ggaatgtttt tggcagacat ggtgactacc ttcatctggg
     9121 tgagttcagt tgatttgtct tgagcctttg gggtttacac aagtaaatga catcatacag
     9181 ttagtgtatt gttagtgaat attaatatat gaggcaggct ttgctctagc aattttagaa
     9241 ctagttttca ggaaagggtt catcttgtgc attggatgtt tgattctatc acttagagtt
     9301 taaactgaaa gtgctcaaga ggttttattt aggctgggat ataaataagc ctttctgtag
     9361 cttgtaatgg tatcaggaat ttaaaaggcc atctggggca caaagattaa gcagaaaagg
     9421 tagaaggtga gattggggga ctttgagtac ttcacacact ttaatgtgtg agtgctttag
     9481 tgcatatagt acaactgcca gataagggca tccacatctg attgtttgga aggcaccttg
     9541 tggtttctgg gaattcagaa ttgggagaaa aatgctccca accgctgaag cccttggtaa
     9601 tctgcagggt gtttatttag caggagataa ggacaaaaag ttatagtgtg gagttggttg
     9661 agttggtaga tgtcattaca acaggtggtc ttaaattggg ttaggagtca ctttgaaata
     9721 cctgggccat aagcaaagtg gcattttcac ctttcaggag aaactggtac acttatccat
     9781 tctatagtgc atgcttgttc aattgggctg atgactaaac cggtgactaa aggtttgtca
     9841 gtataaatgg atgggttgta ggcagacggt gaggaattac tatacctgca aggagtcatt
     9901 gcctgatctg cctggaaagg ggcaggattg agtctcagaa ggtgtacacc ataggatatg
     9961 gaaaaatttg tcacgcctag cattcaactt agtggtgtag cgccacctac tggcacttta
    10021 aaagcttagc atagaggagc atgtgtgtta ggagctcgga tgggatccag ggcctcaagg
    10081 tttgcatgta aataaaagcc ctttaccaaa ttaactacat accagcatac atcagtcctt
    10141 tagtgttgaa aaacagaagt gaaagctaat atatatagtg cttgctttat ttaagtctag
    10201 ctgattacgt gtttggttgc cagtgtgact agtctggagt tgaatttgtc ctcagacacg
    10261 taaaatggaa tttgggattc acaacactct agtatgaggg acctaatggc ctgtaccagg
    10321 cacaaacgtg tctataaact acacaaaacg aaggaattta caggaattag gaaggtattc
    10381 ttaacattaa aacattatgg gcattttaaa aaaagctttg acaggatttc tttgtcatgg
    10441 ctgtcctgga gctagttgtg tagaccaggc tgggctgaaa tcttgtctgc ctgcctggct
    10501 tggacacttt tttattatgt atacaacatt ctgcttccat gtatatctgc acattagaag
    10561 acggcaccag atctcctaat ggatggttgt gagccaccat gtggttgctg ggaattgaac
    10621 tcaggacctc tggaagagca gtgctcttaa cctctgagcc atctccagcc ccagcttggg
    10681 cacattttta atggctggga aatcaaaccc cctaggcctt ctgtcagtaa tgaagggctt
    10741 ttggctaccg agagtaggat ttaaggttat tcggagctgc aggtctgcct cagtgcaggt
    10801 ttgggagtcc agcatcttag aaaatgcagt gaagccaagc tgagctatat tttgtttaaa
    10861 aaaaaaataa gtgggtaaag tgctgctgag cctgatgacc aagctgggac acaagtagaa
    10921 gaacataggc caatgctcta tattaaaagc atgggtcatt tttaatgctc ttgagaaggc
    10981 tatgcctaca ctactctcag ccaccgcagc gtgtttaaat taaactagtt tggaaatttt
    11041 ctttgggggt aagctattta acctagtgcc ttggcaggta tactactgaa ctctcctcct
    11101 cattcctttt tgttttttaa gaatttcagt caggctcagg cagcccttaa acttgtgatt
    11161 aagcctgaga acagttacga ttatgagcct attagtatac cgatcaatat gtgaattttt
    11221 ttgggatggg ggtcaggcct ccctgcctcc caaatactgg gactaaaggc tgcaccacca
    11281 caacctggct cttgaaatac ttttctacat tttttggggg gcatgggtgg gagagcaggg
    11341 tttctctgta ttagccctgg ctctcctgga actctgtaga ccaggctatt cttgagctca
    11401 gattagcctg tctctgcctc ctaaattctg ggattaaagg tgtgtgctac tgctgcctgg
    11461 ctacaaagac attttttttt ttcttaaatt taaaaacaaa agtggttctt ttagaagggt
    11521 ggttggtgtt ggcacatact ccaagcactc aggttttgag tttgtcccag gaatgaagac
    11581 tgcattactg ccgcccctcc ctggtaaggg ctacacagag aaatcctatt tggagcctgt
    11641 cctggtaact cgctctgtag accaggctgg cctcgaactc aagagaacca cctgcctctg
    11701 aatgctggta ttaagggcag gcaccaccaa cacccagcct aaaaaatgtc ttttttttaa
    11761 agattttttt tttttttttt acagaataaa cattctgttt acaatattct gcttctatgt
    11821 atatctgcac actagaagag ggcacccgat ctcataatgg atggttgtga gccaccaagt
    11881 ggttgctggg aattgaactc agaacctctg gaagagcagt cagtgctctt aacctctgag
    11941 ccatctctcc agcccctaaa aatggctctt gagatagggt ctcaagtagt ttgagactga
    12001 gttggctata taaacaaggc tggcacatag caccatgtac agctgggttt agtttacatg
    12061 gggtgttttt gtctctggag gcaggaggat catttgagca tagggagtta atagtgaggt
    12121 catgttttat ctactcttct gaattgagaa ctaagctgat gcaaagcaag tttgactgaa
    12181 gaagtccagt ttatgagaac aagggtggaa actaatgtgt caaagatggc cttgcatgtg
    12241 ttttagatga tgacccagtc acttgggaat tactggatgt gtaagaccta tatcttgaca
    12301 ggagtgaaca gtgtcttata ggtcctatat gaaagaaatg agacataccc attttgtttc
    12361 ccctaagaat tcacttttcc taacctggtt catgctattt aggttatttt acttgcaaat
    12421 cctagggtgc tcccttaccc agtattgctt atgtggcacc aaagtcactc actcccatga
    12481 tttgcaagtc tctgggaact tccatgacaa cctagaatag caactcaaat acattttctc
    12541 agtaccaatt ttgaagaaaa aatattttgc aaaatagctg tatggatggg tactaaatag
    12601 tgaggttatc tccagaaggc ctatgaagaa ttaaggttga gttcagttga gttcagcagc
    12661 aagtttaagg ttcatccatt tttgtacagt gttttcctat tacggtaagt gttttgcctg
    12721 caggaatatc tgtaccacat gcttgcctgg tacctatatc ggccagaaga gggctttgga
    12781 tcctctggac ttgaattaca gatgggtatt agccaccatt taggtgctgg gaattgaaac
    12841 caagtcctct ggaagaacag caagtgactc gacgtccgga gctcaggcgc gcctgtggaa
    12901 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    12961 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    13021 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    13081 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    13141 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
    13201 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
    13261 cggatctgat cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
    13321 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
    13381 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
    13441 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
    13501 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
    13561 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
    13621 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
    13681 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
    13741 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
    13801 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
    13861 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
    13921 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
    13981 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
    14041 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
    14101 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
    14161 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
    14221 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
    14281 cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt
    14341 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    14401 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    14461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    14521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtata
//
