LOCUS       sequence-315338-        4413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Lyn11-greenFAST in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Tebo AG, Moeyaert B, Thauvin M, Carlon-Andres I, Boken D, Volovitch 
            M, Padilla-Parra S, Dedecker P, Vriz S, Gautier A
  TITLE     Orthogonal fluorescent chemogenetic reporters for multicolor 
            imaging.
  JOURNAL   Nat Chem Biol. 2020 Aug 10. pii: 10.1038/s41589-020-0611-0. doi: 
            10.1038/s41589-020-0611-0.
  PUBMED    32778846
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Aug 10. pii: 10.1038/s41589-020-0611-0. doi: 
            10.1038/s41589-020-0611-0."
COMMENT     Sequence Label: pAG361-lyn11-greenFAST
FEATURES             Location/Qualifiers
     source          1..4413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             603..977
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDEQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVATGTDSPEFYRKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
     CDS             984..1013
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1141..1262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1178..1197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1232..1251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1269..1724)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1406..1427)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1618..1637
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1751..1855
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1857..2214
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1857..2048
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1880..1900)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2065..2200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2249..3043
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2249..3043
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2303..2322)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2913..2932
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3231..3250)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3275..3322
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3651..4239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4140..4159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctatgggc tgcatcaagt ccaagggcaa ggactccgcc ggcggcggct
      601 ccatggagca tgttgccttt ggcagtgagg acatcgagaa cactctggcc aaaatggacg
      661 acgaacaact ggatgggttg gcctttggcg caattcagct cgatggtgac gggaatatcc
      721 tgcagtacaa tgctgctgaa ggagacatca caggcagaga tcccaaacag gtgattggga
      781 agaacttctt caaggatgtt gcaactggaa cggattctcc cgagttttac cgcaaattca
      841 aggaaggcgt agcgtcaggg aatctgaaca ccatgttcga atggatgata ccgacaagca
      901 ggggaccaac caaggtcaag gtgcacatga agaaagccct ttccggtgac agctattggg
      961 tctttgtgaa acgggtggga tccgaacaaa agcttatttc tgaagaggac ttgtaatagg
     1021 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     1081 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     1141 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1201 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     1261 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     1321 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     1381 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1441 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1501 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1561 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1621 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1681 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     1741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     1861 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     1921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     1981 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2041 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2101 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     2161 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     2221 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     2281 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     2341 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     2401 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     2461 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     2521 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     2581 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     2641 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     2701 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     2761 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     2821 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     2881 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     2941 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3001 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     3061 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     3121 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     3181 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     3241 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     3301 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     3361 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     3421 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     3481 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     3541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4321 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     4381 gtcattagtt catagcccat atatggagtt ccg
//
