LOCUS       sequence-31535-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB31
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             640..657
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(710..728)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      724..771
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(895..914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tgcgatacgg gagctcaaag tgtgccttct
      121 cggggacact ggggttggga aatcaagcat cgtgtgtcga tttgtccagg atcactttga
      181 ccacaacatc agccctacta ttggggcatc ttttatgacc aaaactgtgc cttgtggaaa
      241 tgaacttcac aagttcctca tctgggacac tgctggtcag gaacggtttc attcattggc
      301 tcccatgtac tatcgaggct cagctgcagc tgttatcgtg tatgatatta ccaagcagga
      361 ttcattttat accttgaaga aatgggtcaa ggagctgaaa gaacatggtc cagaaaacat
      421 tgtaatggcc atcgctggaa acaagtgcga cctctcagat attagggagg ttcccctgaa
      481 ggatgctaag gaatacgctg aatccatagg tgccatcgtg gttgagacaa gtgcaaaaaa
      541 tgctattaat atcgaagagc tctttcaagg aatcagccgc cagatcccac ccttggaccc
      601 ccatgaaaat tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      661 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      721 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      781 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      901 tcgctttctt cccttccttt ctcgccacgt tcgccggc
//
