LOCUS       sequence-31536-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-SRM
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     275..294
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     378..397
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        378..396
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    397..421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             436..458
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             477..494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             507..527
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccctgattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
       61 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
      121 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
      181 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
      241 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
      301 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
      361 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
      421 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
      481 atcatcatca tcacagcagc ggcagagaaa acttgtattt ccagggcagc aaaaaatggt
      541 tctccgaatt ttcgatcatg tggccggggc aagccttcag cctgaagatc aaaaaaatcc
      601 tgtacgagac caagtccaaa taccagaacg tcctggtgtt cgagtcgacg acctacggca
      661 aagtcttggt gcttgacgga gtaattcagc tgactgaaaa ggacgagttt gcataccacg
      721 agatgatgac gcatgtccca atgacagtta gcaaagagcc gaaaaacgtg ctcgtagttg
      781 gtgggggaga tggcggtata attcgtgaat tatgcaagta taaatccgtg gagaacatcg
      841 acatctgcga gattgacgag actgtcattg aggtgagtaa aatctacttc aagaacatca
      901 gctgcggcta cgaggacaaa cgcgtc
//
