LOCUS       sequence-315360-        8612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-off lentivirus construct all-in-one vector, with Blast 
            resistance, for expression of ALDOA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T,
            Lewis CA, Sabatini DM
  TITLE     Dihydroxyacetone phosphate signals glucose availability to mTORC1.
  JOURNAL   Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. 
            Epub 2020 Jul 27.
  PUBMED    32719541
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0250-5"; journalName: "Nat Metab"; date: 
            "2020-09"; volume: "2"; issue: "9"; pages: "893-901"
COMMENT     Sequence Label: 3XFLAG_ALDOA in pCW57_tTA_Blast
FEATURES             Location/Qualifiers
     source          1..8612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..390
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    84..102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    120..138
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    298..316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     363..387
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      407..416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             416..481
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        1613..2123
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2042..2060
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2133..2525
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2535..2588
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2589..3206,3207..3212,3213..3248,3249..3251,3252..3287,
                     3288..3290,3291..3326,3327..3335)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3375..3395)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3793..3804)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4009..4242
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4314..4435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4351..4370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4405..4424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4475..4610
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4610..4681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4682..4750,4751..5542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4900..4919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5713..6301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6202..6221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6455..6472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6589..6610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6625..6630,6631..6648,6649..6655)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6663..6679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6668..6690
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6687..6703
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6687..6703
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6722..6742
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6724..6742
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6770..6996
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6997..7177
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7224..7349
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7842..8075
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8260..8304
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(8517..8612,1..22)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
       61 gcgaatcgat atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
      121 ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      241 agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc
      301 ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc
      361 agagctcgtt tagtgaaccg tcagatcgcc tggagaattg gctagcgcca ccatggacta
      421 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
      481 ggatctcaat ggtggtggtg gtgggtcgac gatgccctac caatatccag cactgacccc
      541 ggagcagaag aaggagctgt ccgacatcgc tcaccgcatc gtggcacctg gcaagggcat
      601 cctggctgca gatgagtcca ctgggagcat tgccaagcgg ctgcagtcca ttggcaccga
      661 gaacaccgaa gagaaccggc gcttctaccg ccagctgctg ctgacagctg acgaccgcgt
      721 gaacccctgc attgggggtg tcatcctctt ccatgagaca ctctaccaga aggcggatga
      781 tgggcgtccc ttcccccaag ttatcaaatc caagggcggt gttgtgggca tcaaggtaga
      841 caagggcgtg gtccccctgg cagggacaaa tggcgagact accacccaag ggttggatgg
      901 gctgtctgag cgctgtgccc agtacaagaa ggacggagct gacttcgcca agtggcgttg
      961 tgtgctgaag attggggaac acaccccctc agccctcgcc atcatggaaa atgccaatgt
     1021 tctggcccgt tatgccagta tctgccagca gaatggcatt gtgcccatcg tggagcctga
     1081 gatcctccct gatggggacc atgacttgaa gcgctgccag tatgtgaccg agaaggtgct
     1141 ggctgctgtc tacaaggctc tgagtgacca ccacatctac ctggaaggca ccttgctgaa
     1201 gcccaacatg gtcaccccag gccatgcttg cactcagaag ttttctcatg aggagattgc
     1261 catggcgacc gtcacagcgc tgcgccgcac agtgcccccc gctgtcactg ggatcacctt
     1321 cctgtctgga ggccagagtg aggaggaggc gtccatcaac ctcaatgcca ttaacaagtg
     1381 ccccctgctg aagccctggg ccctgacctt ctcctacggc cgagccctgc aggcctctgc
     1441 cctgaaggcc tggggcggga agaaggagaa cctgaaggct gcgcaggagg agtatgtcaa
     1501 gcgagccctg gccaacagcc ttgcctgtca aggaaagtac actccgagcg gtcaggctgg
     1561 ggctgctgcc agcgagtccc tcttcgtctc taaccacgcc tattaaggat ccggggttgg
     1621 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     1681 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     1741 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     1801 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     1861 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     1921 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     1981 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     2041 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     2101 gaatcaccga cctctctccc cagcaattca ccatggttcc tttgtctcaa gaagaatcca
     2161 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     2221 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     2281 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     2341 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     2401 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     2461 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     2521 agggccccgg tgccgaaggt agaggttctc tcctcacttg tggtgatgtt gaagaaaacc
     2581 ctggtccaat gtctagactg gacaagagca aagtcataaa ctctgctctg gaattactca
     2641 atgaagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     2701 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctggcaatcg
     2761 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     2821 ttctgcggaa caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta
     2881 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     2941 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     3001 gccactttac actgggctgc gtattggagg atcaggagca tcaagtagca aaagaggaaa
     3061 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     3121 accatcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     3181 agaaacagct aaagtgcgaa agcggcgggc cggccgacgc ccttgacgat tttgacttag
     3241 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     3301 ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcga tccaagatat
     3361 cgtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3421 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3481 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3541 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3601 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3661 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     3721 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3781 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3841 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3901 gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt acctttaaga ccaatgactt
     3961 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4021 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4081 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4141 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4201 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     4261 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     4321 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4381 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4441 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4501 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4561 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tttcaaatat
     4621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5041 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5641 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5701 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5761 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5821 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5881 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5941 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6001 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6061 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6541 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6601 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     6661 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     6721 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     6781 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     6841 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     6901 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     6961 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     7021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7141 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7201 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7261 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7321 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7381 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7441 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7501 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7561 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7621 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7681 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7741 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7801 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7861 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7921 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7981 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     8041 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     8101 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     8161 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     8221 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     8281 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     8341 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     8401 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     8461 ccaaccccga ggggacaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
     8521 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     8581 acagacatac aaactaaaga attacaaaaa ca
//
