LOCUS       sequence-315369-        5405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0024:Dendra
FEATURES             Location/Qualifiers
     source          1..5405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1349..1372
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1629..2429
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2554..3142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3043..3062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacgagtcc tattcagacc gcgaccgcga ccctgcacac
      121 caaccgcggc gacatcaaga tcgcactgtt cggcaaccac gccccgaaga cggtgaacaa
      181 cttcgtcggc ctggcgcagg gcaccaagga ctacagcacc gagaacgcct cgggtggcac
      241 ctccggcccg ttctacgacg gcgccgtctt ccaccgcgtc atcgacggct tcatgatcca
      301 gggcggtgac cccaccggca cgggtcgtgg cggcccgggt tacaagttcg aggacgagtt
      361 ccaccccgag ctgcagttcg acaagccgta cctgctggcc atggccaacg ccggcccggg
      421 caccaacggc tcgcagttct tcatcaccgt gggcaagacg ccgcacctca accggcgcca
      481 caccatcttc ggtgaggtcg tcgacgagga gtcgcagaag gtcgtcgacg cgatcgcctc
      541 gacgcccacc gaccgtagcg accgtccgac cgagccggtc gtgatcgagt ccatcacgat
      601 cgccaagctt ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat
      661 gcgtgtcaag gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga
      721 gggtaagggt aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc
      781 ccctctccca ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt
      841 caccaagtac cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc
      901 ctgggagcgg acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc
      961 gctggaaggg gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa
     1021 cggcccggtc atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt
     1081 tcgggacggc ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca
     1141 ctacctatgc gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc
     1201 gcacttcgtg gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa
     1261 gttgtacgag cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg
     1321 cggcggcggg gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa
     1381 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc
     1441 gaattcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     1501 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     1561 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg
     1621 atcgactgat gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg
     1681 agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca
     1741 gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc
     1801 gtgctacgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg
     1861 agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat
     1921 tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc
     1981 cacaggcaga gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc
     2041 cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg
     2101 ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac
     2161 actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact
     2221 gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag
     2281 gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc
     2341 tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag
     2401 aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt
     2461 ggaggggcaa ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc
     2521 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2581 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2641 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2701 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2761 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2821 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2881 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2941 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     3001 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3061 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3121 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3181 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3241 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     3301 gtgcggccgc ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt
     3361 gggccacccc gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca
     3421 gcccgccgac ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta
     3481 ttctcttcac tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg
     3541 gggggctcta ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc
     3601 gtatgcccag gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca
     3661 gagggcgaaa acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta
     3721 gtttggaaga atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc
     3781 ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct
     3841 gagagttgca caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg
     3901 gcacgtcgcg gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac
     3961 gtcaacccgc aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac
     4021 gccgaagcct ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca
     4081 caggaccggg cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag
     4141 tggctcgtgg agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag
     4201 cgccgcatct acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg
     4261 cgtgcgtggt gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac
     4321 gtcctccggg cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc
     4381 cggatcgagc agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg
     4441 gacatcgtcg ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg
     4501 acgagcctcc ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc
     4561 atgacgatga agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt
     4621 ggcaacgcca agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag
     4681 atgatccgag cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcattcctg
     4741 gtgaccacga cgcagggcaa ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt
     4801 ggctacgcca agatcggtcg gccggaactc cgcatccacg acctccgcgc tgtcggcgct
     4861 acgttcgccg ctcaggcagg tgcgacgacc aaggagctga tggcccgtct cggtcacacg
     4921 actcctagga tggcgatgaa gtaccagatg gcgtctgagg cccgcgacga ggctatcgct
     4981 gaggcgatgt ccaagctggc caagacctcc tgaaacgcaa aaagcccccc tcccaaggac
     5041 actgagtcct aaagaggggg gtttcttgtc agtacgcgaa gaaccacgcc tggccgcgag
     5101 cgccagcacc gccgctctgt gcggagacct gggcaccagc cccgccgccg ccaggagcat
     5161 tgccgttccc gccagaaatc tagacggtga ccacaacgcg cccgctttga tcggggacgt
     5221 cgaaatattg gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga
     5281 tgatcggccg catccccacg gtgctgcgca gtgagctcta cgccatcccg gcgttgatct
     5341 gtgcgttcgc acgcacaggc ccggtgtgag aagggtctct gacgagcggg agaactccct
     5401 atcag
//
