LOCUS       sequence-315379-        6593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0229:Dendra
FEATURES             Location/Qualifiers
     source          1..6593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2537..2560
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2817..3617
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3742..4330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4231..4250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgccctcaga cgacaagtcc actgagactc ctgacatcaa
      121 gccccgcagt cgcgacgtca ccgacggcct cgagaaggcg gccgcccgtg ggatgctccg
      181 tgcggtaggc atgggcgacg ccgactgggc caagccgcag atcggcgtcg ggtcgtcgtg
      241 gaacgagatc acgccgtgca acatgtcgct gcagcgcctc gcgaagtcgg tcaaggacgg
      301 cgtccacgag gcgggcggct acccgctcga attcggcacc atctcggtgt ccgacggcat
      361 ctcgatgggc cacgagggca tgcacttctc gctggtctcg cgcgaggtga tcgccgacag
      421 cgtcgagacc gtcgtgcagg ccgagcgcct cgacggcacg gtcctgctgg ccggctgcga
      481 caagtccatt ccgggcatgc tcatggctgc cgcgcggctg gacctcgcct cggtgttctt
      541 ctacaacggc tcgatcatgc cgggtgtcgc gaagctgacc gacggcaccg agaaggaagt
      601 cacgatcatc gacgcgttcg aggccgtcgg cgcgtgcgtg cgcggcctga tgtcgcgtga
      661 ggacgtcgac atcatcgagc gcgcgatctg ccccggcgag ggtgcctgcg gcggcatgta
      721 caccgccaac accatggcca gcgccgccga ggccctcggc ctgtcgctgc ccggcagcgc
      781 gtcgccggtc gccatcgaca agcgccgcga cgagttcgcg cgccgctccg gcgaggccgt
      841 cgtcgacatg ctgcgtcgcg gcatcaccgc tcgcgacatc ctcaccaagg aggccttcga
      901 gaacgccatc gccgtggtca tggcgttcgg cgggtccacc aacgccgtgc tgcacctgct
      961 ggcgatcgcc cgcgaggccg aggtggagct gacgctcgac gatttcacgc gcgtcggcag
     1021 caaggtcccg cacctcgcgg acgtcaagcc gttcggccgc catgtcatga agcacgtcga
     1081 cgagatcggc ggtgtgcccg tggtgatgcg cgctctgctg gatgccggtc tgctgaacgg
     1141 cgagtgcctg acggtcacgg gcaagaccat ggccgagaac ctggcccaca tcgctccccc
     1201 ggatcccgac ggcaaggtgc tgcgggccat gaacaacccg atccacccga ccggcggcat
     1261 cacgatcctg cacggctcgc tcgctcccga gggtgcggtg gtcaagtcag ccggcttcga
     1321 ttcggacgtg ttcgagggca ctgcaagggt tttcgaccgc gaacgtgccg cgatggacgc
     1381 gctcgaggac ggcaccatcc aggcgggcga cgtcgtggtg atccgctacg agggccccaa
     1441 gggcggtccc ggtatgcgcg agatgctcgc gatcaccggc gccatcaagg gtgcgggtct
     1501 gggcaaggac gtgctgctga tgaccgacgg tcggttctcg ggcggtacga cgggtctgtg
     1561 cgtcgggcac atcgcgccgg aggccgtcga cggcggcccg atcgcgttcg tgcgcgacgg
     1621 tgaccgcatc cgcctcgacg tcgccaaggg cacgctcgac gtgctcgtcg acgaagaaga
     1681 gttcgaggcc cgcaaggccg gcttcgaacc cctgccgccg cggtacaaga ccggtgtgct
     1741 ggccaagtac accaagctgg tccagtccgc cgccgtcggc gcagtctgca ccaagcttgg
     1801 tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt
     1861 gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa
     1921 gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt
     1981 ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc
     2041 cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac
     2101 gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga
     2161 ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat
     2221 gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct
     2281 cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga
     2341 cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga
     2401 ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca
     2461 cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga
     2521 gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg
     2581 ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag
     2641 ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg
     2701 ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata
     2761 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     2821 catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     2881 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     2941 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg
     3001 gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc
     3061 ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     3121 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     3181 agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg
     3241 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     3301 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     3361 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     3421 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     3481 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     3541 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     3601 ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg
     3661 tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt
     3721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4141 aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4381 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4441 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg
     4501 tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt
     4561 tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg
     4621 cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg
     4681 caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt
     4741 cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt
     4801 cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac
     4861 accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat
     4921 gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca
     4981 tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca
     5041 ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga
     5101 tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag
     5161 gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg
     5221 ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg
     5281 aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag
     5341 cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac
     5401 ccggtgctag gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg
     5461 gccgggatgg gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg
     5521 gtgatgaaca cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag
     5581 aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc
     5641 gctgagatct tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg
     5701 ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag
     5761 ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag
     5821 accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg
     5881 cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg
     5941 cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag
     6001 atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct
     6061 caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg
     6121 gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc
     6181 aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa
     6241 agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc
     6301 cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc
     6361 cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga
     6421 tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca
     6481 tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac
     6541 gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat cag
//
