LOCUS       sequence-315383-        5744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0384:Dendra
FEATURES             Location/Qualifiers
     source          1..5744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1688..1711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1968..2768
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2893..3481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3382..3401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcgcggcat catcctcgcc ggcggatccg gtaccaggct
      121 gcaccccctc accatcggcg tcagcaagca attgctcccg gtgtacgaca agccgctcgt
      181 ctactacccg ctgtccacgc tgatcatggc cggcatccgg gacattctgg tgatcaccac
      241 ccccgctgac gcaccggctt ttcgaagact cctcggcgac ggctcggatt tcggtgtcaa
      301 cctcagctac gccgcacaga atgagcccga aggccttgcc caggcatttc tgatcggcgc
      361 cgatcacatc ggcaacgaca cggtcgcact tgccctgggg gacaacatct tctacggacc
      421 aggattgggc acgagcctgc gtcgcttcga acacgtcagt ggtggggcaa tattcgcgta
      481 ctgggtggca aatccgtccg cttacggtgt ggtcgagttc gatgccgacg ggaaagcggt
      541 atcgctggag gagaagccca agacgccgaa atcccattac gccgtgcccg gcctctactt
      601 ctatgacaac accgtcatcg acatcgcacg atcgctgaag aaatcagctc gcggagaata
      661 cgagatcacc gaggtcaacc agatctacct gaaccgcggc caattgtccg tggaggtgct
      721 ggctcgcggg accgcttggc tcgataccgg cacgttcgat tcgttgttgg atgccagcga
      781 tttcgtccgg acgatcgaac tgcgtcaggg cctgaaagtc ggcgcacccg aggagatcgc
      841 gtggcgcgcg ggattcatcg atgacgatca actcgcgaca cgcgccaagg aactgctgaa
      901 gtcgggctac ggccactacc tgctccaact tctggatcga gagaagcttg gtgcggccgc
      961 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     1021 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     1081 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     1141 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     1201 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     1261 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     1321 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     1381 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     1441 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     1501 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     1561 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     1621 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     1681 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     1741 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     1801 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     1861 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     1921 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     1981 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     2041 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     2101 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     2161 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     2221 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     2281 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     2341 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     2401 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     2461 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     2521 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     2581 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     2641 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     2701 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     2761 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     2821 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     2881 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2941 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3001 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3061 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3121 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3181 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3241 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3301 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3361 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3421 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3481 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3541 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3601 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     3661 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     3721 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     3781 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     3841 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     3901 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     3961 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     4021 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     4081 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     4141 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     4201 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     4261 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     4321 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     4381 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     4441 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     4501 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     4561 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     4621 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     4681 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     4741 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     4801 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     4861 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     4921 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     4981 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     5041 gaccgtacga agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac
     5101 cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg
     5161 ccggaactcc gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt
     5221 gcgacgacca aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag
     5281 taccagatgg cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc
     5341 aagacctcct gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg
     5401 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
     5461 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct
     5521 agacggtgac cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca
     5581 ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg
     5641 tgctgcgcag tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc
     5701 cggtgtgaga agggtctctg acgagcggga gaactcccta tcag
//
