LOCUS       sequence-31541-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cp-CAP1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(610..627)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(637..654)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             673..695
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    710..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(734..753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(735..753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(840..859)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccggatctca gctggtggtg gtggtggtgc tcgagatgcg gccgcangct tcgtnatcat
       61 ccatacaatg gagaaaccat actttctaat ttgcccttac tttcattgta tttagatgac
      121 aaattgttca ggaattgcaa gttctttcca gtcaccttct tcttctcctg gaaagagtaa
      181 attcatttca cttgatttac tgctgtaaac ctcaacattc tttccttctt tggatgtgta
      241 gatattaact ttatttgatt tatcaagaga aatagttgga acaatgcctg tcatttgtaa
      301 tttaatatca tcacaattaa caatttctat ggaagaaatt acaccattta ctagaaagat
      361 acatccaaca caattttcaa taaaaatact gttgactttt tgttcaacaa taattctgct
      421 attttgagag catctaactg aaaccttgtt tttaatactt ccttctgata atgtaatagg
      481 atcagcgcaa tttacatgat tttctacaag atatgtatcc ttttgtaatt ccacctttgg
      541 actaccattt gtaactactt gtcttgctgc tttcattttc ttttcttttt tcaaataaat
      601 ttgcgattta ctaccacgcg gaaccaggcc gctgctgtga tgatgatgat gatggctgct
      661 gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      721 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc ggggatcgag atctcgatcc
      781 tctacgccgg acgcatcgtg gccggcatca ccggcgccac agggtgcggt ttgctggcgc
      841 ctatatcgcc gacatcaccg atggggaaga
//
