LOCUS       sequence-315412-        2281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 HSPt
FEATURES             Location/Qualifiers
     source          1..2281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3..60
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(84..101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(255..843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(335..354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1026..1120)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1141..1800)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1715..1734
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1962..1980
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1984..2027)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
       61 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      241 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
      301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     1021 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     1081 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1141 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1201 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1261 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1321 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1381 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1441 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1501 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1561 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1621 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1681 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1741 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1801 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1861 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
     1921 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1981 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcggtct
     2041 ctgcttatat gaagatgaag atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt
     2101 aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa
     2161 atgaatgtaa gatctcatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt
     2221 tttgttggat ctcttctgca gcatataact actgtatgtg ctatggcgct tgagaccctg
     2281 c
//
