LOCUS       sequence-315413-        2271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 UBQ5t
FEATURES             Location/Qualifiers
     source          1..2271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      35..92
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(116..133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(287..875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(367..386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1058..1152)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1173..1832)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1747..1766
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1994..2012
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2016..2059)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tgatattcat tggttgccgc ttgagaccct gcagtccggc aaaaaagggc aaggtgtcac
       61 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
      121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      721 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1021 tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc
     1081 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1141 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1201 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1261 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1321 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1381 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1441 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1501 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1561 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1621 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1681 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     1741 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     1801 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     1861 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1921 cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1981 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
     2041 tttcgctaag gatgatttct ggaattcggt ctctgctttc tgttgtagcg gtagatcgat
     2101 ccttctcttt ctctcttatc ttttaaaaac tgcatttcta tttgggaatt ttgtaagctc
     2161 tttaatttga gttatcatgg attctatgtt gaacatcttt cgttctggat tataaattat
     2221 ggaaggtttt aggattttat ggatattcat tatgttgttc gttctatact t
//
